Phytochemical characterization, antioxidant activity, and in vitro investigation of antimicrobial potential of Dittrichia viscosa L. leaf extracts against nosocomial infections

Mssillou, Ibrahim; Agour, Abdelkrim; Slighoua, Meryem; Tourabi, Meryem; Nouioura, Ghizlane; Lyoussi, Badiâa; Derwich, Elhoussine

doi:10.1016/j.chnaes.2021.09.021

Cited by 22 publications

(28 citation statements)

References 64 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The study performed by Mssillou et al [ 33 ] on the antimicrobial effect of ethanolic and acetonic extracts of D. viscosa against nosocomial infections caused by Escherichia coli, Bacillus subtilis, and Staphylococcus aureus appears more efficient compared to our study. An explanation for these results could be linked to extraction solvent and/or the resistance of the used bacterial strains [ 33 ]. In the present study, D. viscosa showed high levels of flavonoids and polyphenols such as hispidulin, cirsiliol, and caffeic acid derivatives.…”

Section: Resultscontrasting

confidence: 56%

Dittrichia viscosa L. Leaves: A Valuable Source of Bioactive Compounds with Multiple Pharmacological Effects

et al. 2022

View full text Add to dashboard Cite

This work focused on the leaves of Dittrichia viscosa, a plant used in Mediterranean folk medicine. Compared to water extract, the methanolic extract had higher antioxidant effects. Moreover, this extract showed potent in vitro inhibitory activity against α-amylase and α-glucosidase and showed an interesting antiglycation effect. Additionally, the evaluation of the cytotoxic activity of the methanolic extract against two human breast cancer cell lines, MCF-7 and MDA-MB-468, was very promising, with no cytotoxicity towards normal cells (peripheral blood mononuclear cells (PBMCs). The antibacterial effect was also assessed and showed potent inhibitory activity against Proteus mirabilis and Bacillus subtilis. On the other hand, Dittrichia viscosa leaves were rich in macro-elements containing appropriate micro-elements and high levels of phenolics and flavonoids such as caffeic acid derivatives. Taken together, the results obtained in this study indicate that Dittrichia viscosa could constitute a valuable source of bioactive molecules and could be used either on the preventive side or for therapeutic applications without toxicity.

show abstract

Section: Resultscontrasting

confidence: 56%

Dittrichia viscosa L. Leaves: A Valuable Source of Bioactive Compounds with Multiple Pharmacological Effects

et al. 2022

View full text Add to dashboard Cite

show abstract

“…A high negative correlation between TFC (R = −0.82) in Prunus persica L ( Mokrani and Madani, 2016 ) , TPC (R = −0.791) in Dittrichia viscosa L. ( Mssillou et al, 2021 ) and DPPH scavenging activity revealed that the lower TFC and TPC in plant extracts contributed to the higher DPPH radical scavenging activity. The present findings are in line with previous research showing negative correlations between TPC, TFC and DPPH scavenging activity in B. acmella extracts.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Analysis, Antioxidant and Bone Anabolic Effects of Blainvillea acmella (L.) Philipson

et al. 2022

View full text Add to dashboard Cite

Blainvillea acmella (L.) Philipson [Asteraceae] (B. acmella) is an important medicinal plant native to Brazil, and it is widely known as a toothache plant. A plethora of studies have demonstrated the antioxidant activities of B. acmella and few studies on the stimulatory effects on alkaline phosphatase (ALP) secretion from bone cells; however, there is no study on its antioxidant and anabolic activity on bone cells. The study aimed to evaluate the phytochemical contents of aqueous and ethanol extracts of B. acmella using gas chromatography mass spectrometry (GCMS) and liquid chromatography time of flight mass spectrometry (LCTOFMS) along with the total phenolic (TPC) and flavonoid (TFC) contents using Folin-Ciocalteu and aluminum colorimetric methods. The extracts of B. acmella leaves were used to scavenge synthetic-free radicals such as 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2′-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and ferric reducing antioxidant power (FRAP) assays. The bone anabolic effects of B. acmella extracts on MC3T3-E1 cells were measured with 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazoium bromide (MTT) at 1, 3, 5, and 7 days, Sirius-red and ALP at 7 and 14 days, and Alizarin Red S at 14 and 21 days. Comparatively, ethanol extract of B. acmella (BaE) contributed higher antioxidant activities (IC50 of 476.71 µg/ml and 56.01 ± 6.46 mg L-ascorbic acid/g against DPPH and FRAP, respectively). Anabolic activities in bone proliferation, differentiation, and mineralization were also higher in B. acmella of ethanol (BaE) than aqueous (BaA) extracts. Positive correlations were observed between phenolic content (TPC and TFC) to antioxidant (ABTS and FRAP) and anabolic activities. Conversely, negative correlations were present between phenolic content to antioxidant (DPPH) activity. These potential antioxidant and bone anabolic activities in BaE might be due to the phytochemicals confirmed through GCMS and LCTOFMS, revealed that terpenoids of α-cubebene, cryophyllene, cryophyllene oxide, phytol and flavonoids of pinostrobin and apigenin were the compounds contributing to both antioxidant and anabolic effects in BaE. Thus, B. acmella may be a valuable antioxidant and anti-osteoporosis agent. Further study is needed to isolate, characterize and elucidate the underlying mechanisms responsible for the antioxidant and bone anabolic effects.

show abstract

“…In the preparation of the extracts (BCEE and BCAE), 100 g of the powder of the aerial parts (ground by a Waring ® blender, New Hartford, CT, USA) was macerated in 1 L of ethanol (Sigma-Aldrich, St. Louis, MO, USA) at 70% for 48 h, while the aqueous extract was prepared by infusion (100 g of the powder in 1 L of boiling water). The filtrates were dried at 37 °C until the total removal of the solvent and then stored at 4 °C [ 23 ].…”

Section: Materiel and Methodsmentioning

confidence: 99%

Extracts of Brocchia cinerea (Delile) Vis Exhibit In Vivo Wound Healing, Anti-Inflammatory and Analgesic Activities, and Other In Vitro Therapeutic Effects

Agour

Mssillou

Barnossi

et al. 2023

Life

Self Cite

View full text Add to dashboard Cite

The plant Brocchia cinerea (Delile) (B. cinerea) has many uses in traditional pharmacology. Aqueous (BCAE) and ethanolic extracts (BCEE) obtained from the aerial parts can be used as an alternative to some synthetic drugs. In vitro, DPPH, FRAP and TAC are three tests used to measure antioxidant efficacy. Antibacterial activities were determined against one Gram positive and two Gram negative strains of bacteria. The analgesic power was evaluated in vivo using the abdominal contortion model in mice, while carrageenan-induced edema in rats was the model chosen for the anti-inflammatory test; wound healing was evaluated in an experimental second degree burn model. The results of the phytochemical analysis showed that BCEE had the greatest content of polyphenols (21.06 mg AGE/g extract), flavonoids (10.43 mg QE/g extract) and tannins (24.05 mg TAE/g extract). HPLC-DAD reveals the high content of gallic acid, quercetin and caffeic acid in extracts. BCEE has a strong antiradical potency against DPPH (IC50 = 0.14 mg/mL) and a medium iron reducing activity (EC50 = 0.24 mg/mL), while BCAE inhibited the growth of the antibiotic resistant bacterium, P. aeruginosa (MIC = 10 mg/mL). BCAE also exhibited significant pharmacological effects and analgesic efficacy (55.81% inhibition 55.64% for the standard used) and the re-epithelialization of wounds, with 96.91% against 98.60% for the standard. These results confirm the validity of the traditional applications of this plant and its potential as a model to develop analogous drugs.

show abstract

Phytochemical characterization, antioxidant activity, and in vitro investigation of antimicrobial potential of Dittrichia viscosa L. leaf extracts against nosocomial infections

Cited by 22 publications

References 64 publications

Dittrichia viscosa L. Leaves: A Valuable Source of Bioactive Compounds with Multiple Pharmacological Effects

Dittrichia viscosa L. Leaves: A Valuable Source of Bioactive Compounds with Multiple Pharmacological Effects

Phytochemical Analysis, Antioxidant and Bone Anabolic Effects of Blainvillea acmella (L.) Philipson

Extracts of Brocchia cinerea (Delile) Vis Exhibit In Vivo Wound Healing, Anti-Inflammatory and Analgesic Activities, and Other In Vitro Therapeutic Effects

Contact Info

Product

Resources

About