Phytochemical, Anti-inflammatory and Analgesic Studies of the Crude Ethanolic Leaf Extract of Globimetular brounii Van Tieghem (Family: Loranthaceae)

Abstract

Globimetula braunii is a bushy parasitic plant growing on Terminalia catappa, which has a variety of use in African traditional medicine. The leaves of the plant was subjected to preliminary phytochemical analysis, Acute Toxicity studies and analgesic activity using Hot plate and acetic acid induced pain method in mice, while the anti-inflammatory activity was tested using Carrageenan-induced paw oedema in rats. The Phytochemical analysis of the ethanolic leaf extract of Globimetula braunii revealed the presence of Carbohydrate, Steriods, Triterpenes, Tannins, Saponins, Alkaloids and Flavonoids; The LD 50 was found to be greater than 5000 mg/kg which shows that the plant extract is relatively safe. The results from the Hot-plate method showed that the extract was statistically significant at (p<0.02 and p<0.01), this shows that there is increase in the latency of pain in the extract compare to standard drug pentazocine (20 mg/kg), while the Acetic acid induced method the extract was statistically significant at (p<0.02) with a percentage of inhibition of 65.5%, 65.2%, and 67.7% at the doses of 12.5 mg/kg, 25 mg/kg, and 50 mg/kg compared with the standard drug piroxicam (10 mg/kg) with a percentage inhibition of 54.3%. The extract was also found to significantly (P<0.05) inhibit oedema at all doses: 250 mg/kg, 500 mg/kg and 1000 mg/kg. This shows that the plant contains phytochemical constituent with analgesic and anti-inflammatory activities. Collection and identification of materials The leaves of G. braunii growing on Terminalia catapa were collected in the month April, 2013, at the fields of Ahmadu Bello University Zaria, Nigeria. The plant was identified by Mallam U.S.

Analgesic and anti-inflammatory studies of the methanol extract of Globimetula braunii (Loranthaceae) growing on Terminalia catappa Linn (Combretaceae)

“…Preliminary phytochemical screening of methanol stem extract of Globimetula braunii growing on Terminalia catappa showed the presence of flavonoids, steroids, triterpenes and saponins (Table 1). The findings of this study is corroborative with previous findings [12,19]. However, in this study alkaloids were found to be absent from the stem part of Globimetula braunii, this might be due to the difference in the part of plant used or the season at which plant was collected [20].…”

“…The plant is used traditionally in the treatment of various ailments such as rheumatic pains [11]. Study conducted [12], reported that, the leaves of Globimetula braunii possess analgesic and anti-inflammatory activities. In our previous study, extensive literature of the plant was reported [13].…”

Analgesic and Anti-Inflammatory Activity of Total Crude Leaf Extract of &lt;i&gt;Phytolacca dodecandra&lt;/i&gt; in Wistar Albino Rats

“…mg/kg bwt (Table 2 &Figure 2). The observed effects could have been due to the similar compounds reported to be found in the medicinal plant[7] [8].…”

“…These plants contain various phytochemical compounds that include phenolic acids, alkaloids, saponins, flavonoids, glycosides, terpenes, lignans, steroids, coumarins, anthraquinones, edotides, xanthones and sesquiterpene lactone [5] [6]. Previous studies have reported that flavonoids, tannins, saponins, phenolic acids and alkaloids present in different medicinal plants have both analgesic and anti-inflammatory activities [7] [8]. Among the medicinal plant used include P. dodecandra.…”

Analgesic activity of the combined extract of sukun (Artocarpus altilis) and melinjo (Gnetum gnemon L.) Leaves in mice

“…There are many types of plants that are reported to contain alkaloids, tannins, saponins which are thought to be used as analgesic and anti-inflammatory drugs such as Anacardium occidentalis [20], Globimetular brounii [21], Papaver libanoticum [22].…”