Phytochemical and Psychotropic Research of Motherwort (Leonurus cardiaca L.) Modified Dry Extracts

Abstract

The prospect of creating a new medicine with psychotropic activity is shown as a result of studying the chemical composition and pharmacological activity of modified dry extracts of motherwort (Leonurus cardiaca L.) tincture. The most promising substances were the dry extracts, modified by adding small amounts of arginine, valine, phenylalanine, glycine, lysine, and alanine. A total of 15 main phenolic substances were found in the extracts, and eight of them were identified. There were also 10 hydroxycinnamic acids in these extracts, three of which were identified (chlorogenic, caffeic, and rosmarinic acids). The dominant hydroxycinnamic acids were chlorogenic and caffeic acids. Among flavonoids, catechin, hyperoside, and rutin were identified. It should be noted that the extracts had a significant content of ellagic acid. On the basis of the results of the phytochemical analysis of the extracts, it can be concluded that the composition of phenolic compounds does not differ significantly, and the main differences are related to amino acids, which obviously have an impact on the overall pharmacological effect. The results obtained indicate the presence of anxiolytic activity in the motherwort extracts studied in complex with amino acids. The extracts with glycine, valine, and arginine were more effective in reducing anxiety in animals.

Highbush Blueberry (Vaccinium corymbosum L.) Leaves Extract and Its Modified Arginine Preparation for the Management of Metabolic Syndrome—Chemical Analysis and Bioactivity in Rat Model

“…Modification of bearberry extract with phenylalanine resulted in more pronounced anti-inflammatory and diuretic activity [ 33 ]. Modification of motherwort tincture with different amino acids allowed to create new substances with stress-protective and anxiolytic properties [ 32 ]. Modification of the bilberry leaves extract with amino acid L-arginine allowed to create a substance with significant hypoglycemic and hypolipidemic activity [ 10 ].…”

“…Therefore, it has become an interesting question, whether the use of such an approach to modify the total plant extract could result in improvement of its biological properties. It was previously shown that the modification of tincture Leonurus cardiaca L. with amino acids has led to the creation of new more active substances with anxiolytic activity [ 32 ]. The modification of the blueberry leaves extract with arginine allowed to create a substance with pronounced hypoglycemic and hypolipidemic activities [ 10 ].…”

Chromato-mass-spectrometric research in Salvia grandiflora L., Salvia pratensis L. and Salvia verticillata L. aboveground organs

“…The NIST14 mass spectrum library was used to identify the components. Quantitative determination of the content of substances in the raw material was performed in comparison with a standard sample of menthol [36,37].…”

Carboxylic acids in the flowers of Veronica spicata L. and Veronica incana L.

“…The reaction mixture was decanted, and the precipitate was diluted in 1 mL of distilled water; methyl esters of carboxylic acids were extracted with 0.2 mL methylene chloride. The mixture was gently shaken several times within an hour and then the obtained extract of the methyl esters was chromatographed [28,29].…”

“…The reaction mixture was decanted, and the precipitate was diluted in 1 mL of distilled water; methyl esters of carboxylic acids were extracted with 0.2 mL methylene chloride. The mixture was gently shaken several times within an hour and then the obtained extract of the methyl esters was chromatographed[28,29].Identification and comparative content determination of carboxylic acidsThe gas chromatography-mass spectrometry (GC-MS) analysis of carboxylic acids was performed on Ag-…”