Phytochemical and pharmacological evaluation of methanolic extract of Lathyrus sativus L. seeds

Abstract

Background: Lathyrus sativus L. (Fabaceae) has long been used as a traditional medicine for the treatment of several ailments such as Scabies, eczema, and allergy. The aim of the study was to evaluate the phytochemical nature with Central Nervous System (CNS) depressant, analgesic, antipyretic activities of the methanolic plant extract of Lathyrus sativus L. seeds in different experimental models. Methods: Preliminary phytochemical screening and proximate analysis was carried out using different standard methods. CNS depressant activity was evaluated observing the effects of plant extract on Swiss albino mice using open field and hole-cross method. Acetic acid induced writhing and formalin induced paw licking methods were used for the appraisal of analgesic activity while 2,4-dinitrophenol (DNP) induced pyrexia model was used to investigate the antipyretic activity. The data were analyzed by one way ANOVA followed by Dunnett's test using SPSS (version 20). Results: The phytochemical analysis revealed the presence of wide range of phytoconstituents in the plant extract. Our investigation demonstrated that the methanolic plant extract significantly (p < 0.001) decreased the locomotor activity of mice in open field and hole-cross method at both the tested doses (200 and 300 mg/kg) which were comparable to the standard drug diazepam (1 mg/kg). The plant extracts significantly (p < 0.001) inhibited the writhing induced by acetic acid in mice to 87.09% and 80.65% (200 and 300 mg/kg respectively) compared to the standard indomethacin (70.97%). The extracts (200 and 300 mg/kg respectively) also significantly (p < 0.001) reduced the writhing to 43.39%, 64.15% in early and 46.15%, 97.44% in late phase of formalin-induced licking and biting. In 2,4-DNP induced pyrexia the extracts exhibited protection at 200 and 400 mg/kg, similar to standard drug aspirin at 150 mg/kg. Conclusion: The results demonstrated that the plant extract has potential CNS depressant, analgesic and antipyretic activity.

Phytochemical and pharmacological activities of methanol extract of Artemisia vulgaris L. leaves

“…Recently, Bhattacharjee et al evaluated the analgesic activity of methanolic extract of Lathyrus sativus seeds at the dose 200 and 300 mg/kg b. wt. and found a very significant inhibition (87.09% and 80.65%) of writhes induced by acetic acid in mice in comparison to indomethacin (70.97%) [44]. Consequently, a similar kind of the finding was made by the Islam et al with extract of Campsis radicans, where he took the doses of 200 and 400 mg/kg b.wt., and proved high analgesic activity [45].…”

Hypoglycemic and in vitro antioxidant activities of Stereospermum kunthianum stem bark hydromethanol extract

“…This study evaluated the hypoglycemic and in vitro antioxidant activities of SKSB extract. The extract contained diverse constituents, including saponins, terpenes, tannins, steroids, flavonoids, carbohydrates and alkaloids, an indication of the richness of the plant in phytochemicals that may have bioactive properties (Bhattacharjee et al, 2018). It has been reported that the common phytoconstituents found in medicinal plants with anti-diabetic potential include polyphenols, flavonoids, terpenoids, tannins, alkaloids, saponins (Mohd et al, 2017).…”

Hybrid Magnetic-Semiconductor Oxides Nanomaterial: Green Synthesis and Environmental Catalytic

“…Lathyrus sativus is a well-known plant that contains alkaloids, carotenoids, flavonoids, starch, carbohydrates, essential oil, leguminvicilin, legumelin, vitamin C, oleoresin, gum resin, tannins, terpenes, phenols, riboflavin, beta-carotene, proteins, and amino acids, which act as capping, reducing and stabilizing agents [17,18]. Thanks to this unique feature, many research groups have used L. sativus shell extract to synthesize magnetite nanoparticles [19,20].…”