Phytochemical And <i>In Vitro</i> Antimicrobial Assay Of The Leaf Extract Of <i>Newbouldia laevis</i>

Abstract

The methanolic leaf extract of Newbouldia laevis was subjected to preliminary phytochemical screening and in-vitro antimicrobial tests. The extract revealed the presence of flavonoids, tannins, terpenes, steroidal and cardiac glycosides. The antimicrobial activity of the plant extract was assayed by the agar plate disc diffusion and nutrient broth dilution techniques. Test microorganisms were Pseudomonas aeruginosa, Escherichia coli, Staphylococcus aureus, Salmonella typhi, Klebsiella spp. and Candida albicans; all the organisms were laboratory isolates. The extract inhibited the growth of all the test organisms especially against Klebsiella spp. and S. aureus which had mean inhibition zone of 42.3±1.5 and 32.3±1.5 mm respectively. The results showed minimum inhibitory concentration (MIC) of 1.563 mg/ml against Escherichia coli and Klebsiella spp. and 3.125 mg/ml against Pseudomonas aeruginosa, Staphylococcus aureus and Salmonella typhi. The minimal bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus was 0.39 mg/ml. This study has justified the traditional use of this plant for the treatment of stomach discomfort, diarrhea, dysentery and as a remedy for wound healing whose causative agents are some of the organisms used in this study.

Phytochemical Screening and Antimicrobial Evaluation of Syzygium aromaticum Extract and Essential oil

“…Syzygium aromaticum constituents (alkaloids, saponins, tannins and flavonoids) obtained in this research are known to have curative activity against several pathogens thus the essential oil and crude extract are hereby recommended for treatment of diseases caused by S. aureus, E. coli, P. aeruginosa and C. albicanss since some of these organisms are known to play a vital role in invasive skin diseases including superficial and deep follicular lesion (Usman and Osuji, 2007).…”

Phytochemical and Antibacterial Evaluations of the Stem Bark of <i>Newbouldia laevis</i> against Isolates from Infected Wounds and Eyes

“…In South Eastern and part of the Midwestern Nigeria, the plant is used for the treatment of septic wounds and eye problems [6] . The antimicrobial potential of methanol extract of the leaf has been reported in literature while the antiinflammatory and antimalarial activities of the root extract have also been documented [7,8] . Scientific reports on the phytochemical constituents of the plant revealed the presence of alkaloids and phenylpropanoids in the root [9], flavonoids, and tannins in the leaf [7] .…”

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“…However, it may be suggested that plant extracts exhibiting diameter of zones of inhibition greater than 10mm is considered as active (Usman and Osuji, 2007). Thus it is believed that plant extracts is better antimicrobial agents for various pathogenic bacteria.…”