Phytochemical and Antibacterial Evaluations of the Stem Bark of &lt;i&gt;Newbouldia laevis&lt;/i&gt; against Isolates from Infected Wounds and Eyes

Akerele, JO; Ayinde, BA; Ngiagah, J

doi:10.4314/tjpr.v10i2.66566

Cited by 25 publications

(22 citation statements)

References 13 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Usman and Osuji (2007) did not detect the presence of alkaloids and saponins in their study while Dandjesso et al (2012) reported the absence of alkaloids, flavonoids, saponins and steroids on the leaf extract. Ejele et al (2012) reported in their work the absence of flavonoids and steroids while Akerele et al (2011) reported the presence of saponins and steroids in the stem extract but was not detected in this study. Differences in these reports could be due to environmental factors, time of collection and handling.…”

Section: Discussioncontrasting

confidence: 44%

Investigation of some important phytochemical, nutritional properties and toxicological potentials of ethanol extracts of Newbouldia laevis leaf and stem

Anaduaka¹,

Emeka²,

Ogugua³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

The pytochemicals, nutritional and toxicological potentials of the ethanol extracts of the leaf and stem of Newbouldia laevis was investigated in this study. The percentage yields of N. laevis ethanol leaf and stem extracts were found to be 7.44 and 3.30% (w/w), respectively. The preliminary phytochemical screening showed that ethanol leaf and stem extracts contains alkaloids, flavonoids and tannins. The quantitative phytochemical analysis showed that the leaf and stem extracts contained respectively: alkaloids (14.74 ± 0.06 and 6.27 ± 0.0 mg/g), flavonoids (15.51 ± 0.04 and 5.18 ± 0.04 mg/g), cardiac glycosides (6.77 ± 0.02 mg/g), tannins (1.74 ± 0.11 mg/g), saponins (4.07 ± 0.06 mg/g), steroids (41.72 ± 0.02 mg/g) and terpenoids (8.67 ± 0.09 mg/g). The following amounts of vitamins and minerals were found in the leave and stem-bark extracts, respectively; vitamin A (5.19 ± 0.00 and 3.01 ± 0.00 mg/100 g), vitamin C (2.35 ± 0.55 and 1.05 ± 0.08 mg/100 g) and vitamin E (9.33 ± 0.02 and 4.08 ± 0.11 mg/100 g); minerals: Mg (76.12 ± 0.04 and 54.25 ± 0.04 mg/100 g), Fe (16.84 ± 0.06 and 1.19 ± 0.03 mg/100 g) and Se (3.08 ± 0.03 and 0.29 ± 0.07 mg/100 g). The acute toxicity test of the ethanol leaf and stem extracts showed no toxicity up to 5000 mg/kg body weight.

show abstract

Section: Discussioncontrasting

confidence: 44%

Investigation of some important phytochemical, nutritional properties and toxicological potentials of ethanol extracts of Newbouldia laevis leaf and stem

Anaduaka¹,

Emeka²,

Ogugua³

et al. 2013

Afr. J. Biotechnol.

View full text Add to dashboard Cite

show abstract

“…The zone of inhibition produced by standard antibiotic disc (ciprofloxacin) against test organisms were found to be appreciable in relation to those activities of extracts produced by most organisms studied and it was statistically significant. This conforms to studies conducted by Usman and Osuji [7], Odunbaku and Amusa [15] and Akerele et al [10].…”

Section: Discussionsupporting

confidence: 93%

“…The method described by Akerele et al [10] with slight modification was used for the extraction procedure 250g of the powdered leaf was extracted in 100ml (70%) ethanol and water respectively using maceration method for 48 hours. The crude extract was decanted and filtered using Whatman No.…”

Section: Extraction Proceduresmentioning

confidence: 99%

<i>In Vitro</i> Evaluation of Ethanolic and Aqueous Crude Extracts of <i>Newbouldia laevis</i> Leaves on Bacterial Isolates from Ear Infections

Kanu

Kalu

2018

IJPPE

View full text Add to dashboard Cite

Abstract. The antibacterial effects of ethanolic and aqueous crude extract of leaves of Newbouldia laevis were evaluated against pathogenic Staphylococcus aureus, Staphylococcus epidermidis, Bacillus spp, Pseudomonas aeruginosa and Escherichia coli isolated from ear infections. A concentration gradient of the ethanolic and aqueous extract (12.5 mg/ml -100 mg/ml) was prepared and its effectiveness was tested by agar well diffusion method and nutrient broth dilution technique. The organisms tested varied in pattern of susceptibility but were more sensitive at high concentrations. The zone of inhibition of aqueous extract of Newbouldia laevis on test organisms ranged from 4.0 to 15 mm while the ethanolic extracts of Newbouldia laevis on test organisms ranged from 6.5 to 21.00 mm. The comparative susceptibility of test bacteria to Newbouldia and ciprofloxacin showed that there was a significant difference in the antibacterial activity of leaf extract and the antibiotic standard. The MIC values ranged from 12.5 mg/ml to 25 mg/ml. The extract showed a higher antibacterial activity against E. coli, Bacillus spp and S. epidermidis. The result of the study suggests that the leaf extract of N. laevis has the potential and could be used as a source for new broad spectrum antibiotics to treat ear infections caused by test organisms.

show abstract

“…Prior to analyses, samples were cleansed with distilled water; air dried at room temperature and ground into fine powder using mortar and pestle. Alkaloid, tannin, flavonoid and saponin contents were quantitatively evaluated using methods described by Akerele et al [35] while the analysis of phenol, anthraquinone and cyanogenic glycosoides followed Krishnaiah et al, Soladoye and Chekwuma, Piero et al [36,37,38].…”

Section: Laboratory Investigationmentioning

confidence: 99%

Evaluation of Phytochemical and Mineral Constituents of Piper guineense Schum. & Thonn. and Piper Umbellatum Linn: Implications for Ethnomedicine

Isikhuemen

Ogbomwan

Efenudu

2020

EJMP

View full text Add to dashboard Cite

Aims: A significant number of tropical rainforest shrubs and lianes are inextricably linked to human use as food, spices and/or medicine. This study evaluates phytochemical and mineral constituents and ethnomedicinal uses of Piper guineense Schum. & Thonn and Piper umbellatum Linn. Methods: Two phased investigations – ethnomedicinal uses and phytochemical/mineral constituents of P. guineense and P. umbellatum – were carried out in Benin City, Edo State, Nigeria. Firstly, key informants’ interview and questionnaire were used to elicit reactions from respondents (vendors and end users) on ethnomedicinal and sundry uses of P. guineense and P. umbellatum in nine purposively selected markets out of 27. Secondly, samples of leaves and stems of P. guineense and P. umbellatum were collected and analysed in the laboratory for selected phytochemicals (alkaloid, cyanogenic glycosides, tannin, flavonoid, tannins, anthraquinones, saponin and phenol), and minerals (Ca, Mg, Na, K, P, Fe, Zn). Results: Market survey revealed that vendor/end users (85%) were well-informed on the uses of the plants for food (spices, condiments) and ethnomedicine. Most respondents (80%) affirmed that the leaves were frequently used for flavouring; and for preparing traditional medicinal soups when combined with assorted vegetables. Respondents (80%) averred that the leaf was the most frequently used followed by stems (10.9%), roots (5.5%) and seeds (3.6%). P. guineense was considered best for stomach disorder/ulcer, respiratory/urinary tract infections; P. umbellatum most preferred for arthritis, fever and fertility/womb cleansing. Phytochemical analysis revealed P. umbellatum stem had more alkaloids, flavonoids and phenol; and anthraquinone in the leaf while P. guineense stem had more saponins and tannins. However, mineral analyses revealed more Ca and Mg in P. guineense leaf; and K, Na, Zn and P in P. umbellatum stem while Fe was higher in the leaf. Conclusion: P. guineense and P. umbellatum are aromatic liane and shrub used for flavouring and spicing of food, and medicine. These fairly ubiquitous and underutilized plants can contribute significantly to food and health needs of forest dependent people in Nigeria and elsewhere in West Africa. The study has revealed that these aromatic plants are rich sources of phytomedicines and important minerals. Further investigation on the phytochemical/pharmacological potentials and ecological idiosyncrasies of these underutilized aromatic rainforest liane and shrub is recommended.

show abstract

Phytochemical and Antibacterial Evaluations of the Stem Bark of <i>Newbouldia laevis</i> against Isolates from Infected Wounds and Eyes

Abstract: Purpose: To examine the phytochemical constituents and verify the ethnomedical claim of Newbouldia laevis (P.Beauv.) Seeman ex Bureau

Cited by 25 publications

References 13 publications

Investigation of some important phytochemical, nutritional properties and toxicological potentials of ethanol extracts of Newbouldia laevis leaf and stem

Investigation of some important phytochemical, nutritional properties and toxicological potentials of ethanol extracts of Newbouldia laevis leaf and stem

<i>In Vitro</i> Evaluation of Ethanolic and Aqueous Crude Extracts of <i>Newbouldia laevis</i> Leaves on Bacterial Isolates from Ear Infections

Evaluation of Phytochemical and Mineral Constituents of Piper guineense Schum. & Thonn. and Piper Umbellatum Linn: Implications for Ethnomedicine

Contact Info

Product

Resources

About