Phytochemical and GC–MS analysis of bioactive compounds of Sphaeranthus amaranthoides Burm

Background:Clerodendrum viscosum is commonly found in India and Bangladesh. Previously, various parts of this plant were reported for treatment of different types of diseases and there was no report on GC-Ms analysis.Objective:To analyze and characterize the phytochemical compounds of methanol extract of Clerodendrum viscosum using GC-MS.Materials and Methods:The preliminary phytochemical screening of methanol extract was carried out according to standard procedures described in WHO guidelines. Various bioactive compounds of the extract were determined by GC-MS technique.Results:The presence of steroids, triterpenoids, alkaloids, saponins, flavonoids, tannins and carbohydrate was found on phytochemical screening of methanol extract of the leaves. The GC-MS analysis showed 16 peaks of different phytoconstituents namely acetamide, N, N-carbonylbis-, 4-Pyranone,2,3-dihydro-, alpha-D-Galactofuranoside, methyl 2,3,5,6-tetra-O-methyl-, Glycerin, Xylitol, N, N-Dimethylglycine, 4H-Pyran-4-one,2,3-dihydro-3, 5-dihydroxy-6-methyl-, Benzofuran,2,3-dihydro-, 5-Hydroxymethylfurfural, 2(1H)Pyrimidinone,1-methyl-, 2,4-Dihydroxy-5,6-dimethylpyrimidine, 3-Deoxy-d-mannoic lactone, 1,3-Methylene-d-arabitol, Orcinol, n-Hexadecanoic acid and Phenol,4,4’-(1-methyl ethylidene) bis etc.Conclusion:The bioactive compounds present in the methanol extract of Clerodendrum viscosum suggest the application of this extract for the treatment of various diseases by the aborigine tribes.

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“…[ 2 ] Various plants still available in the nature are yet to be explored for their medicinal potential. [ 3 ]…”

Section: Introductionmentioning

confidence: 99%

GC-MS analysis of bioactive compounds in the methanol extract of Clerodendrum viscosum leaves

et al. 2015

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“…In many studies increase in the number of morphological defects of head, neck and tail of the spermatozoa is associated with Tobacco smoking, [22]. Sphaeranthus amaranthoides is used by traditional practitioners for its anti inflammatory action due to the presence of various bioactive compounds [23]. It is evident that nicotine always produces degenerative changes in the male reproductive system [24].…”

Section: Discussionmentioning

confidence: 99%

Histological Changes in Testis of Adult Wistar Albino Rats - Nicotine-Induced Testicular Damages and the Protective Effects of Methanolic Extract of Sphaeranthus Amaranthoides

Kanimozhi

Sumathy

Prithiviraj

2021

JPRI

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Nicotine which is the most dynamic substance present in tobacco is the vital cause of damaging the tissues and oxidative stress that in turn is the principal cause of numerous diseases. The succession and severity of nicotine toxicity which may be adjourned by the natural antioxidants may cause serious health, knock. We have scrutinized the consequences of methanolic extract of Sphaeranthus amaranthoides (SAE) on nicotine-induced reproductive toxicity, oxidative damage, and hepatotoxicity in adult Wistar male albino rats. In four groups 24 rats were contingently split up. Control, Nicotine N (0.4 mg/kg. i.p) methanolic extract of Sphaeranthus amaranthoides (SAE) and (N+ SAE) group. In order to compute the levels of testosterone and to measure the hematological and oxidative stress parameters, blood samples were collected after 48 days. The reproductive organs were weighed and taken for semen analysis and histopathology study. We could see decrease in the levels of testosterone, weights of reproductive organs, and even in the morphological features of sperms due to reproductive toxicity of Nicotine. On histological examination, testis showed atrophy, degenerative alterations of spermatogenesis in numerous seminiferous tubules together with increased interstitial spaces and a decrease in the number of Leydig cells. SAE played a defensive role against nicotine which persuaded the reproductive effects by enhancing the spermatogenesis, semen quality, and testicular histological damage.

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“…The sample was introduced via an all-glass injector working in the split mode, with a helium (99.9% purity) carrier gas flow rate of 1.2 mL.min -1 . The identification of compounds was accomplished by comparison of retention time, peak area percentage, and fragmentation pattern, as well as with mass spectra of the GC-MS. 32 The identification of compounds from the spectral data was based on the available mass spectral records (NIST.17 and WILEY libraries).…”

Section: Gc-ms Analysis Of Bioactive Compoundsmentioning

confidence: 99%

Ultrasound-Assisted and Microwave-Assisted Extraction, GC-MS Characterization and Antimicrobial Potential of Freeze-dried L. camara Flower

Gowda¹,

Chennappa²,

Anusha³

et al. 2022

J. Pure Appl. Microbiol.

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Lantana camara is known to have anti-bacterial properties which can be exploited to develop a natural food preservative. There is huge demand for natural preservatives in food industry due to the increased health risks associated with synthetic preservatives, development of effecient extraction methods are essential to retain heat sensitive bioactive compounds. This aim of this study was to compare the performance of microwave-assisted extraction (MAE), ultrasound-assisted extraction (UAE), and conventional solvent extraction (CSE) methods for extraction of freeze-dried lantana flower. The phytochemicals in freeze-dried flowers were characterized by GC-MS analysis and antibacterial properties were tested at different concentrations (50, 100, and 150 µl) against E.coli, Salmonella, and S. aureus. It was evident that the UAE offered the highest yield (64%), followed by MAE (53%) and CSE (49%) with distilled water as solvent. The freeze-dried extract possessed a high amount of tannins (417 µg/g), followed by flavonoids such as catechol (88 µg/g) and quercetin (9.2 µg/g). The antibacterial potential results revealed that only distilled water-based extraction techniques offered positive inhibition zones of 2.0-2.67 mm (MAE), 1.67-2.67 mm (UAE), and 1.67-2.17 mm (CSE) against all three organisms, while the chloroform based extracts had no inhibition effect. The microwave-assisted extract at 150 µl concentration offered a significant inhibitory effect against all three pathogens. The GC-MS profiling of bioactive compounds in flower extract revealed the presence of hexadecanoic acid as the major phytochemical compound in all three extraction techniques. The study revealed that the chloroform extract failed to exhibit an antibacterial effect due to the absence of alkaloids, saponins, and anthraquinones as a result of its neutralizing effect.

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Phytochemical and GC–MS analysis of bioactive compounds of Sphaeranthus amaranthoides Burm

Cited by 14 publications

References 11 publications

GC-MS analysis of bioactive compounds in the methanol extract of Clerodendrum viscosum leaves

GC-MS analysis of bioactive compounds in the methanol extract of Clerodendrum viscosum leaves

Histological Changes in Testis of Adult Wistar Albino Rats - Nicotine-Induced Testicular Damages and the Protective Effects of Methanolic Extract of Sphaeranthus Amaranthoides

Ultrasound-Assisted and Microwave-Assisted Extraction, GC-MS Characterization and Antimicrobial Potential of Freeze-dried L. camara Flower

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