Phytochemical and Anti-diarrhoeal Properties of Methanolic Leaf Extract of Maerua crassifolia Forssk

Akuodor, Godwin Christian; Ibrahim, Jemilat Aliyu; Akpan, Joseph Linus; Okorie, Abednego; Ezeokpo, Basil Chukwuma

doi:10.9734/ejmp/2014/11246

Cited by 10 publications

(14 citation statements)

References 20 publications

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“…[34][35][36] M. subcordata appears to possess marked antidiarrheal effects, which could be due to the possible presence of gut-relaxant constituent(s). In line with our findings, Akuodor et al 12 reported antidiarrheal effects of the methanol-leaf extract of a similar species, M. crassifolia, with a proposed mechanism of action linked partly to direct inhibitory effects on the propulsive movement of GI-tract smooth muscle. 12 Further tests that were intended to explain the possible mechanism(s) interestingly show that the plant extract showed biphasic effects: at lower doses (0.01-0.1 mg/mL), the extract demonstrated a stimulant effect on the basal contractions of spontaneously contracting rabbit jejuna, followed by antispasmodic effect at higher doses (0.3-1 mg/mL).…”

Section: Discussionsupporting

confidence: 92%

Multiple Pathway–Mediated Gut-Modulatory Effects of Maerua subcordata (Gilg) DeWolf

Palla¹,

Sibhat

Karim

et al. 2020

JEP

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Background Gastrointestinal disorders are often poorly managed, especially in developing countries, where there are limited resources and therapeutic options. Despite the rich diversity of medicinal plants that offer effective treatment options with fewer side effects, studies that provide scientific verification are lacking. Maerua subcordata (Gilg) DeWolf is among the plants claimed to have wide traditional medicine, use, including as a remedy against gastrointestinal problems. Therefore, this work aimed to evaluate the gut-modulatory effects of a crude leaf extract of M. subcordata (MSL.Cr), as well as its possible mechanism of action. Methods A castor oil (10 mL/kg)–induced diarrheal mouse model was used to evaluate the antidiarrheal effect of MSL.Cr, and the spasmodic/antispasmodic effect of the extract was assessed using isolated rabbit jejunum with and without addition of standard cholinergic agonists/antagonists to predict the possible mechanism of action. Results MSL.Cr exhibited 40% and 80% protection against castor oil–induced diarrhea in mice at doses of 500 and 1,000 mg/kg, respectively. In isolated rabbit jejunum, the extract increased spontaneous contractions at low doses (0.01–0.1 mg/mL), and was sensitive to atropine, whereas it showed complete inhibition at higher doses (0.3–1 mg/mL). It was shown that the relaxant effect was possibly mediated by the involvement of phosphodiesterase-enzyme inhibition and K + -channel activation. The extract potentiated the control concentration–response curve of carbachol, shifting it to the left, similarly to the control drug papaverine. The potassium-channel opening–like activity of MSL.Cr was possibly mediated by the involvement of aspecific K + -channels inhibition, since tetraethylammonium, anunselective antagonist of K + channels, significantly reversed its inhibitory effect. Conclusion This study showed that the M. subcordata leaf extract demonstrated gut-modulatory effects, possibly mediated by a combination of muscarinic-receptor stimulation, phosphodiesterase inhibition, and aspecific K + -channel activation.

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Section: Discussionsupporting

confidence: 92%

Multiple Pathway–Mediated Gut-Modulatory Effects of Maerua subcordata (Gilg) DeWolf

Palla¹,

Sibhat

Karim

et al. 2020

JEP

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“…These results suggest that the ethanol leaf extract of P. kotschyi contain antidiarrheal properties. Previous report on the phytochemical screening of P. kotschyi leaves has shown the presence of alkaloids, tannin, cardiac glycosides, steroids, flavoniods and saponins (Akuodor et al, 2014). Plants containing these secondary metabolites have been reported to possess antidiarrhoeal activities.…”

Section: Discussionmentioning

confidence: 99%

“…The rats were sacrificed 30 min later and gastrointestinal tract removed. The distance travelled by the marker (charcoal meal) from the pylorus to caecum was measured and expressed as the percentage of the total length of the small intestine (Abere et al, 2010;Akuodor et al, 2014).…”

Section: Intestinal Transit Testmentioning

confidence: 99%

“…The incidence of diarrhoeal disease remains high despite the efforts of various governments and international organizations to prevent it. Thus it becomes imperative to identify and investigate available natural products as alternative to currently used antidiarrhoeal agents, which are not free from adverse effects (Akuodor et al, 2014). Medicinal plants have been the most accessible form of therapy for the less privileged in the global population (Espinosa et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

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Antidiarrhoeal and antioxidant properties of ethanol leaf extract of Pseudocedrela kotschyi

Essiet¹,

Christian²,

Ogbonna³

et al. 2016

J App Pharm Sci

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This study was carried out to establish the antidiarrhoeal and antioxidant properties of the ethanol leaf extract of Pseudocedrela kotschyi in wistar albino rats. The effect of the ethanol extract on castor oil induced diarrhoea, motility of the GIT using the charcoal plug method and castor oil induced intestinal fluid accumulation in rats were evaluated. The antioxidant potential of the leaf extract was investigated by measuring its capability for scavenging 2, 2-diphenyl-1-picrylhydrazyl (DPPH) radical. The phytochemical constituents and the oral acute toxicity of ethanol leaf extract were also determined in rats. Generally, the ethanol leaf extract at all doses used, was found to posses significant (P<0.05) concentration dependent antidiarrhoeal, antimotility and antienteropooling activity. The leaf extract also exhibited strong antioxidant activity. The phytochemical studies revealed the presence of alkaloids, tannin, cardiac glycosides, steroids, flavoniods and saponins. The LD50 in rats was above 5000 mg/kg. The ethanol leaf extract of Pseudocedrela kotschyi has demonstrated strong antidiarrhoeal, antimotility, antienteropooling and antioxidant activities, supporting previous claims of its traditional use in the treatment of different diseases.

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“…In traditional medicine, the leaves are used for the treatment of some diseases including analgesic, anti-inflammatory, antipyretic, antibacterial and antioxidant, antimalarial, Phytochemical and antidiarrhoea Chilaka et al, 2015;Akuodor et al, 2014a;Akuodor et al, 2014d). In other pharmacological studies, the leaf extract has shown to have effects against gastrointestinal and toothache diseases (Rahman et al, 2004;Idris-Usman et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Haematological and biochemical changes after exposure to Maerua crassifolia ethanol leaf extract in rats

2017

J App Pharm Sci

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This study was carried out to determine the safety profile of Maerua crassifolia by conducting the acute and subacute toxicological assay of its ethanol leaf extract in rats. The animals were treated daily with the leaf extract at doses of 100, 200 and 400 mg/kg orally for 14 and 28 days. Rats used as control were given distilled water and all rats weighed every 7 days. After the test, haematological and biochemical parameters as well as their relative organs weight were determined. There were no significant changes in body weight of treated animals compared to control in both studies. In the acute toxicity test, the extract did not cause any signs of toxicity or produce mortality in rats. Subacute results showed that oral administration of the leaf extract did not significantly (p<0.05 affect their food consumption, haematological and biochemical parameters and relative organs weight. Findings in this study showed that Maerua crassifolia is endowed with the potential beneficial effects as immune booster and protecting the liver and kidney systems.

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Phytochemical and Anti-diarrhoeal Properties of Methanolic Leaf Extract of Maerua crassifolia Forssk

Cited by 10 publications

References 20 publications

<p>Multiple Pathway–Mediated Gut-Modulatory Effects of <em>Maerua subcordata</em> (Gilg) DeWolf</p>

<p>Multiple Pathway–Mediated Gut-Modulatory Effects of <em>Maerua subcordata</em> (Gilg) DeWolf</p>

Antidiarrhoeal and antioxidant properties of ethanol leaf extract of Pseudocedrela kotschyi

Haematological and biochemical changes after exposure to Maerua crassifolia ethanol leaf extract in rats

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