Phytochemical Analysis of Tasmannia lanceolata Extracts and Inhibition of Giardia duodenalis Proliferation

Rayan, Paran; Matthews, Ben; Donnell, Pauline Mc; Cock, Ian Edwin

doi:10.5530/pj.2016.3.19

Cited by 16 publications

(34 citation statements)

References 29 publications

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“…lanceolata extracts were also screened for their ability to inhibit Giardia duodenalis growth (Figure 6). In agreement with previous reports, 14 to the untreated control). The ethyl acetate berry (100% inhibition compared to the negative control), leaf (90% inhibition compared to the negative control) and stem extracts (71% inhibition compared to the negative control) were also very effective at inhibiting trophozoite growth.…”

Section: Anti-giardial Activitysupporting

confidence: 94%

“…14,22 For each test, aliquots of the trophozoite suspension (70 µL) containing approximately 1×10 5 trophozoites were added to the wells of a 96 well plate. A volume of 30 µL of the test extracts or the vehicle solvent or culture media (for the negative controls) was added to individual wells and the plates were incubated anaerobically at 37 o C for 8 hrs in a humidified anaerobic atmosphere.…”

Section: Evaluation Of Anti-giardial Activitymentioning

confidence: 99%

“…Berry and leaf T. lanceolata extractions are potent inhibitors of Giardia duodenalis growth 14 and thus have potential in the treatment of giardiasis. Furthermore, leaf extracts have anti-cancer activity against a panel of carcinoma cell lines.…”

Section: Introductionmentioning

confidence: 99%

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An upscaled extraction protocol for Tasmannia lanceolata (Poir.) A.C. Sm.: Anti-bacterial, anti-Giardial and anticancer activity

Vallette¹,

Rabadeaux²,

Sirdaarta³

et al. 2016

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Background: Tasmannia lanceolata is an endemic Australian plant with a high anti-oxidant capacity. Liquid solvent extractions of T. lanceolata inhibit bacterial growth and block proliferation of several carcinomas and the gastrointestinal parasite Giardia duodenalis. Despite these promising therapeutic properties, methods for the rapid extraction of large quantities of T. lanceolata are lacking. This study aimed to develop a rapid supercritical extraction method to produce extracts which retain therapeutic propertyes and phytochemistry characteristics. Materials and Methods: T. lanceolata fruit and leaf were extracted by both solvent maceration extraction and supercritical fluid extraction (SFE). The extracts were tested for the ability to inhibit bacterial and G. duodenalis growth. Inhibition of CaCo2 and HeLa cancer cells was evaluated using MTS-based colorimetric cell proliferation assays. Toxicity was evaluated using an Artemia franciscana nauplii bioassay and GC-MS headspace analysis was used to evaluate phytochemical similarity between the extracts. Results: T. lanceolata berry and leaf SFEs displayed strong bacterial growth inhibitory activity against bacterial triggers of autoimmune inflammatory diseases, with efficacies similar to the smaller scale liquid solvent extractions. The growth inhibition of the berry SFE was particularly noteworthy against P. mirabilis and K. pneumoniae, with MIC values of approximately 160 and 190 µg/mL, respectively. The berry and leaf SFE extracts also had similar antiproliferative potencies against G. duodenalis (492 and 375 µg/mL, respectively), CaCo2 (4133 and 3347 µg/mL, respectively) and HeLa carcinomas (2652 and 3497 µg/mL, respectively) to those determined for the corresponding liquid solvent extractions. GC-MS analysis of the berry SFE revealed similar terpenoid components and similar abundances to those in liquid solvent berry extraction. Furthermore, all SFEs were either non-toxic or of only low toxicity in the Artemia franciscana toxicity assay. Conclusion: The T. lanceolata SFE retained the tested therapeutic properties, were nontoxic and had similar phytochemical profiles as smaller scale liquid solvent extractions. Thus, SFE is a viable method of rapidly extracting large masses of T. lanceolata plant material to produce quality extracts which retain therapeutic properties.

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Section: Anti-giardial Activitysupporting

confidence: 94%

Section: Evaluation Of Anti-giardial Activitymentioning

confidence: 99%

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An upscaled extraction protocol for Tasmannia lanceolata (Poir.) A.C. Sm.: Anti-bacterial, anti-Giardial and anticancer activity

Vallette¹,

Rabadeaux²,

Sirdaarta³

et al. 2016

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“…Anti-Giardial activity of the K. senegalensis extracts was assessed by direct enumeration of parasite numbers in the presence or absence of extract 33,34 For each test, aliquots of the trophozoite suspension (70µL) containing approximately 1 x 10 5 trophozoites were added to the wells of a 96 well plate. A volume of 30µL of the test extracts or the vehicle solvent or culture media (for the negative controls) was added to individual wells and the plates were incubated anaerobically at 37 o C for 8hours in a humidified anaerobic atmosphere.…”

Section: Evaluation Of Anti-giardial Activitymentioning

confidence: 99%

An examination of the Antimicrobial and Anticancer Properties of Khaya senegalensis (Desr.) A. Juss. Bark Extracts

Rabadeaux¹,

Vallette²,

Sirdaarta³

et al. 2017

Phcog J

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Background: Khaya senegalensis (Desr.) A. Juss. is a common component of the pharmacopeia's of multiple African groupings which inhabit the areas in which it grows. Amongst these groups there is a myriad of medicinal uses in the treatment of a wide variety of bacterial, fungal and protozoal infections, as well as in the treatment of cancers. This study was undertaken to test K. senegalensis bark extracts for the ability to inhibit microbial and cancer cell growth, and thus to validate traditional African medicinal usage of this plant in treating a variety of diseases. Materials and Methods: K. senegalensis bark powder was extracted by both solvent maceration and subcritical fluid extraction (SFE). The extracts were tested for the ability to inhibit bacterial and G. duodenalis growth. Inhibition of Caco-2 and HeLa cancer cells was evaluated using MTS-based colorimetric cell proliferation assays. Toxicity was evaluated using an Artemia franciscana nauplii bioassay and GC-MS headspace analysis was used to identify phytochemical components. Results: K. senegalensis bark extracts displayed strong inhibitory activity against bacterial triggers of several autoimmune inflammatory diseases. The growth inhibitory activity of the methanolic and subcritical extracts was particularly noteworthy against P. mirabilis (MIC values of 185 and 211µg/mL, respectively against the reference strains). These extracts were similarly potent growth inhibitors of K. pneumoniae and A. baylyi, and were moderate inhibitors (MIC >1000µg/mL) of P. aeruginosa and S. pyogenes growth. The methanolic and subcritical K. senegalensis extracts were also potent inhibitors of G. duodenalis (187 and 328µg/mL, respectively), as well as Caco-2 (268 and 470µg/mL, respectively) and HeLa carcinomas (155 and 174µg/mL, respectively). GC-MS analysis of the SFE extract revealed relative abundances of a variety of mono-and sesquiterpenoids. Furthermore, all K. senegalensis bark extracts were non-toxic in the Artemia franciscana toxicity assay, indicating their safety for therapeutic use. Conclusion: These studies validate traditional African therapeutic usage of K. senegalensis in the treatment of microbial infections, autoimmune inflammatory diseases and some cancers.

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“…[11][12][13][14][15] Indeed, these studies have highlighted several high antioxidant fruits as having particular interest, with MIC values for the crude extracts similar to those of conventional antibiotics. Several of the same high antioxidant fruits also have potent anti-Giardial 16,17 and anticancer activities. 18 Despite the relative wealth of information on the medicinal and functional food properties of edible fruits and berries, there have been relatively few studies examining the therapeutic potential of edible nuts.…”

Section: Introductionmentioning

confidence: 99%

Aleurites moluccanus (l.) Willd. Extracts Inhibit the Growth of Bacterial Triggers of Selected Autoimmune Inflammatory Diseases

Mpala¹,

Chikowe²,

Cock³

2017

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Introduction: Aleurites moluccanus (L.) Willd. is a large tree with a wide global distribution. All parts of the tree have been used medicinally and the nut is consumed in a variety of cuisines. Despite this, A. moluccanus nut extracts have not been rigorously examined growth inhibitory properties against many bacteria, including the bacterial triggers of autoimmune inflammatory diseases. Methods: The antimicrobial activity of A. moluccanus nut solvent extractions was investigated by disc diffusion and growth time course assays against a panel of bacterial triggers of autoimmune diseases. The growth inhibitory activity was further quantified by MIC determination. Toxicity was determined using the Artemia franciscana nauplii bioassay. Results: Methanolic and aqueous A. moluccanus nut solvent extracts were potent inhibitors of all of the bacterial triggers of autoimmune diseases screened in this study. The methanolic extract displayed the most potent bacterial growth inhibitory activity. It was particularly potent against the bacterial triggers of rheumatoid arthritis (MICs of 438 and 215 µg/mL against reference and clinical Proteus mirabilis strains; MIC of 187 µg/mL against Proteus vulgaris). The methanolic extract was also a good inhibitor of K. pneumoniae and S. pyogenes growth with MICs < 1000 µg/mL. The aqueous and ethyl acetate extracts were also potent bacterial growth inhibitors, albeit with slightly higher MIC values. The antibacterial activity of the methanolic and aqueous A. moluccanus nut extracts were further investigated by growth time course assays which showed significant growth inhibition in cultures of P. mirabilis, K. pneumpniae and S. pyogenes within 1 h of exposure. All extracts were determined to be nontoxic in the Artemia franciscana nauplii bioassay, indicating their safety for prophylactic use in preventing these autoimmune inflammatory diseases. Conclusions: The lack of toxicity of the A. moluccanus nut extracts and their growth inhibitory bioactivity against the bacterial triggers of rheumatoid arthritis, ankylosing spondylitis and rheumatic heart disease indicate their potential in the development of new therapies targeting the onset of these diseases.

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Phytochemical Analysis of Tasmannia lanceolata Extracts and Inhibition of Giardia duodenalis Proliferation

Cited by 16 publications

References 29 publications

An upscaled extraction protocol for Tasmannia lanceolata (Poir.) A.C. Sm.: Anti-bacterial, anti-Giardial and anticancer activity

An upscaled extraction protocol for Tasmannia lanceolata (Poir.) A.C. Sm.: Anti-bacterial, anti-Giardial and anticancer activity

An examination of the Antimicrobial and Anticancer Properties of Khaya senegalensis (Desr.) A. Juss. Bark Extracts

Aleurites moluccanus (l.) Willd. Extracts Inhibit the Growth of Bacterial Triggers of Selected Autoimmune Inflammatory Diseases

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