Phytochemical Analysis, Antioxidant and Anti-Inflammatory Activity of Calyces from Physalis peruviana

Abstract

Physalis peruviana calyces are used extensively in folk medicine. The crude ethanolic extract and some fractions of calyces were evaluated in order to explore antioxidant and anti-inflammatory activities. The anti-inflammatory activity was evaluated by the TPA-induced ear edema model. The antioxidant in vitro activity was measured by means of the superoxide and nitric oxide scavenging activity of the extracts and fractions. The butanolic fraction was found to be promising due to its anti-inflammatory and antioxidant activities.Therefore, a bio-assay guided approach was employed to isolate and identify rutin (1) and nicotoflorin (2) from their NMR spectroscopic and MS data. The identification of rutin in calyces of P. peruviana supports the possible use of this waste material for phytotherapeutic, nutraceutical and cosmetic preparations.

Matrix effects of the hydroethanolic extract and the butanol fraction of calyces fromPhysalis peruvianaL. on the biopharmaceutics classification of rutin

“…BF was concentrated to dryness under vacuum at 40 °C . Previous works of our group identified rutin as the main of two compounds elucidated in HEE and BF (NMR and MS spectroscopy) . For this research, the content of rutin in both preparation was confirmed by a previously validated HPLC‐UV method.…”

“…Recently, our research group described the antioxidant, anti‐inflammatory and hypoglycaemic activity for HEE and for its butanol fraction (BF). Therefore, in view of the promising pharmacological effects of HEE and BF, the solubility and permeability issues of its main secondary metabolite (rutin), and the probable influence of the whole chemical composition of extract and fraction on these properties, this work aimed to evaluate the matrix effects of HEE and BF on the biopharmaceutics classification of rutin.…”

Dry Powder Formulation From Physalis Peruviana L. Fruits Extract With Antidiabetic Activity Formulated via Co-Spray Drying

“…For this determination, 5 g of material was weighed and placed through a funnel positioned at a fixed height on a graph paper flat horizontal surface and allowing this form a cone, measuring the height (h) and radius (r). The tangent of the angle of repose is given by the expression h/r ratio [8]. Five repetitions were done in triplicate for a total of fifteen data.…”

“…The hygroscopicity showed values between 8.84 % and 15.61 %. Experiments 3,4,7,8,11,12,15 and 16 were the particles with the higher proportion of colloidal silicon dioxide that showed the lowest hygroscopicity and this was the factor with the main influence in this variable (Factor F) (table 3). For high concentrations of colloidal silicon dioxide, the extract could be trapped within these solid particles and then it could be almost unavailable for water adsorption [44].…”

“…It is well known that daily consumption of raw fruits of P. peruviana helps to maintain stable glucose level in blood [3][4][5][6]. Several other uses, some of them supported by pharmacological studies, have been attributed to calyxes [7,8], leaves [3], roots [9], and even for the whole plant [2,[10][11][12][13]. Recently, we described peruvioses A-F as the main compounds present in the sticky exudate of the fruits, and the responsible for the observed in vitro α-amylase inhibition activity [14].…”