Phytochemical Analysis and In-vitro Anti-Diabetic and Anti- Inflammatory study of root extract of Apama siliquosa LAMK.

Abstract

The demand for herbal medicines in many pharmaceutical sectors is growing at a drastic rate due to their improved pharmacological actions, minimal side effects and cost-effectiveness. Anti-inflammatory phytochemicals are found to be efficacious against the treatment of inflammatory diseases like rheumatoid arthritis, glomerulonephritis, hepatitis, inflammatory bowel disease, etc. Anti-diabetic phytochemicals are found to treat the increasing incidence of diabetes prevalent globally. This work aims to perform the phytochemical screening and to evaluate the antidiabetic and anti-inflammatory activity of crude extract of Apama siliquosa Lamk. The method employed for obtaining the active principles includes soxhlation technique with methanol as solvent. The anti-inflammatory property was studied in vitro using inhibition of albumin denaturation technique as well as heat-induced hemolysis and IC50 value was found to be 39.5μg/ml and 36.30μg/ml respectively. The anti-diabetic activity was estimated using the alpha-amylase inhibition assay and Glucose diffusion inhibitory study. The IC50 value for alpha-amylase inhibition assay was found to be 15.75μg/ml. It also shows a strong inhibition of glucose across the dialysis membrane.

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“…All the four extract AESS (Aqueous extract of Spermadicyton suaveolens), EESS (Ethanolic extract of Spermadicyton suaveolens), CESS (Chloroform extract of Spermadicyton suaveolens) and PESS (Petroleum ether extract of Spermadicyton suaveolens) were screened for in-vitro potential Phytochemical Analysis: The preliminary investigation of secondary metabolite in the ethanolic root extract was confirmed by performing qualitative analysis as per standard protocol. Simply by the visual aids like a colour change or frothing is a signal for the presence or absence of secondary metabolites 15,16,17 .…”