Phytochemical analysis and Evaluation of hepatoprotective effect of Maytenus royleanus leaves extract against anti-tuberculosis drug induced liver injury in mice

Abstract

Background: Myrin®-p Forte is an anti-tuberclosis agent that can cause hepatic injuries in clinical settings. Maytenus royleanus (Celastraceae) is a medicinal plant, possesses antioxidant and anticancer activities. The hepatoprotective effect of the methanol extract of Maytenus royleanus leaves (MEM) against Myrin®-p Forte induced hepatotoxicity in mice was investigated.Methods: Mice were randomly parted into six groups (n = 6). Fixed-dose combination of Myrin®-p Forte (13.5 mg/ kg Rifampicin, 6.75 mg/kg Isoniazid, 36.0 mg/kg Pyrazinamide and 24.8 mg/kg Ethambutol; RIPE] was administered for 15 days to induce liver injury. In treatment groups MEM (200 mg/kg and 400 mg/kg doses) and Vitamin B6 (180mg/kg) were administered prior to RIPE. Control group received 2% DMSO. Serum liver function tests, DNA damage, tissue antioxidant enzymes and histopathological alterations were studied. HPLC analysis was performed to determine the chemical composition using standard compounds.(Continued on next page) Results: The quercitin, gallic acid, luteolin, viteixin, apigenin, kaempherol, hyperoside and myricetin contents of all samples were determined by reverse-phase HPLC. Quercetin (0.217 mg/g dry weight) and luteolin (0.141 mg/g dry weight) were the major flavonoids identified in MEM. Myrin®-p Forte markedly (p < 0.05) deteriorated lipid profile and upregulated the concentration of LDH, AST, ALP, ALT and γ-GT in serum along with DNA fragmentation (37.13 ± 0.47%) and histopathological injuries in hepatic tissues of mice compared with the control group. Myrin®-p Forte increased (p < 0.001) lipid peroxidation and H 2 O 2 while decreased (p < 0.001) the activity level of CAT, SOD, POD, GPx, GST, GSR, γ-GT and GSH. Co-administration of MEM (200 mg/kg; 400 mg/kg) or the vitamin B6 (180 mg/ kg) to Myrin®-p Forte administered mice significantly ameliorated LDL, cholesterol, HDL and triglyceride content. Furthermore, MEM dose dependently corrected serum liver function tests, decrease % DNA fragmentation (17.82 ± 0.35 and 7.21 ± 0.32 respectively), DNA damage. MEM treated protect RIPE induced oxidative damage by enhancing antioxidants to oxidants balance. Histological examination comprehends biochemical findings. Conclusion: The antioxidant effects of MEM exerted the hepatoprotective potential against the Myrin®-p Forte induced hepatotoxicity in mice.

Protective efficiency of parsley (Petroselinum crispum) against oxidative stress, DNA damage and nephrotoxicity induced with anti- tuberculosis drugs

“…It was suggested to inhibit lipid peroxidation and scavenge hydroxide and superoxide radicals, thus improving the antioxidant battery, the active constituents present in PAE such as flavonoids and vitamin C. In this context, the protective role of PAE may be due to the antiperoxidant and antioxidant effects of its components. These findings are in line with the latest reports (Al-Seeni et al, 2018& Elkomy et al, 2020 In addition, the current study pointed out that the administration of ATB resulted in a substantial increase in the serum level of inflammatory cytokines (TNF alpha & IL-1β) as well as the percentage of DNA fragmentation; this finding is agonised by recent data from Wali et al (2020) and Shabbir et al (2020). TNF alpha and IL-1β are active pro-inflammatory cytokines and are significant mediators of damage to inflammatory tissue.…”

Hepatoprotective Effect of Corm of Ensete ventricosum (Welw.) Cheesman Extract against Isoniazid and Rifampicin Induced Hepatotoxicity in Swiss Albino Mice

“…In a metaanalysis, it was revealed that rifampicin increased the prevalence of drug-induced liver injury in a multidrug regimen from 1.6% to 2.55% in adults [14]. Hepatotoxicity attributed to anti-TB drugs has been reported 5-34.9% in people treated with anti-TB drugs [15,16].…”

Effects of Methoxyfenozide-Loaded Fluorescent Mesoporous Silica Nanoparticles on Plutella xylostella (L.) (Lepidoptera: Plutellidae) Mortality and Detoxification Enzyme Levels Activities

“…The detoxification enzyme activities in P. xylostella samples treated with methoxyfenozide TC and Me@FL-SiO 2 NPs were observed in a Corning ® 96 Well Clear Polystyrene Microplate, after preparing the homogenates following the methodology [ 38 , 39 ]. About five third-instar of P. xylostella larval populations were crushed with mortar pestle and homogenized on ice in 1.5 mL of 0.1 M PBS (pH 7.8), comprising 1 mM phenyl thio urea (PTU), 1 mM phenylmethyl sulfonyl fluoride (PMSF), 1 mM dithiothreitol (DTT), and 1 mM ethylene diamide tetra acetic acid (EDTA).…”