Phytochemical analysis and assessment of Antioxidant properties of black tea extract obtained from <i>Camellia sinensis</i>

Sarkar, Dhrubajyoti; Debnath, Subhashis; Bose, Santanu

doi:10.5958/0974-360x.2020.00800.8

Cited by 5 publications

(1 citation statement)

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“…22 , Escherichia coli and Klebsiella 23 , Staphylococcus aureus 24 . Additionally, antioxidant activities that can help human in preventing some diseases were studied much on plant extracts [25][26][27][28] whereas microbial extracts were not investigated in both the depth and width.…”

Section: Introductionmentioning

confidence: 99%

Detection of Antimicrobial, Antioxidant and Cytotoxicity Activities of Fusarium oxysporum F01 Isolated from Catharanthus roseus Collected in Vietnam

Tu¹,

Thuy²,

Vinh³

et al. 2021

J. Pure Appl. Microbiol.

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The ability of endophytic fungi to produce valuable bioactive compounds when surviving in medicinal herbs. Finding out endophytic fungi originated from Catharanthus roseus with antimicrobial, antioxidant and cytotoxicity activities is important for pharmaceutical development. The isolation was based on the morphology of fungi. Identification was performed by sequencing 18S rRNA that was compared with known genes using Blast search in the combination of phylogenic analysis by using Clustal W and PhyML in GenomeNet. For the antimicrobial test, the agar diffusion method was used. DPPH scavenging assay for the antioxidant activity determination by using spectrophotometry. The cytotoxicity test was carried out by Sulforhodamine B method. LC-MS was applied for predicting components. We isolated the Fusarium oxysporum F01 strain originated from Catharanthus roseus. The aqueous extracts showed inhibition against Staphylococcus aureus ATCC 25923, Pseudomonas aeruginosa ATCC 27853 (15.00 ± 0.50 mm), Serratia marcescens ATCC 14756 (9.00 ± 0.87 mm), Vibrio parahaemolyticus ATCC 17802 (15.50 ± 0.87 mm), Escherichia coli ATCC 25922 (12.50 ± 0.50 mm). The antioxidant activity of the extract obtained from the supernatant was determined with an IC50 value of about 11 µg/mL. The extract also showed cytotoxicity effect on liver cancer cell line (HepG2) and breast cancer cell line (MCF-7) with the percentage of inhibition of 84.47 ± 3.18 and 94.69 ± 1.59, respectively. LC-MS was used to point out the presence of pratol, a melanogenesis agent. The study provided more interesting information about Fusarium oxysporum F01 isolated in Catharanthus roseus grown in Vietnam, contributing to pharmaceutical sources in the world.

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Section: Introductionmentioning

confidence: 99%

Detection of Antimicrobial, Antioxidant and Cytotoxicity Activities of Fusarium oxysporum F01 Isolated from Catharanthus roseus Collected in Vietnam

Tu¹,

Thuy²,

Vinh³

et al. 2021

J. Pure Appl. Microbiol.

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Evaluation of Sertraline as an antioxidant, anti-inflammatory and anticataract

Gaikwad

Nimbalkar

Sabale³

et al. 2021

RJPT

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Sertraline can also protect against environmental causes of free radicals such as smoking. Cigarette tar is a source of free radicals which has been found to damage erythrocyte membranes. It was also found that Sertraline and its conjugate metabolites could protect erythrocytes from the membranous damage that is caused by smoking. The ability of Sertraline is claimed to exert many beneficial effects on health, including protection against various diseases such as osteoporosis, lung cancer, and cardiovascular disease. The studies showed that there has been a reduction in the risk of cardiovascular disease in subjects, who had a high intake of flavonoids. Progressive disorder of the lung parenchyma and airways or also known as chronic obstructive pulmonary disease (COPD) which happens to be the third-leading cause of death in the USA. Therapies thus far for COPD, unfortunately, is said to be partially effective with possibilities of side effects.

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Anticancer Activity of Microwave Assisted Polyphenolic Compounds Extracted from Combinations of Curcuma Longa and Camellia Sinensis against Lung Cancer Cell Line

Jeba,

Sandhya,

Das

et al. 2023

RJPT

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Objectives: Anticancer activity of microwave assisted polyphenolic compounds was extracted from combinations of Curcuma longa and Camellia sinensisby phytochemical analysis. Invitro anticancer activity was determined by using cell line. Methods: By preliminary phytochemical analysis and using uv-visible spectrophotometer total phenolic content was determined. In vitro anticancer activity was found out by MTT assay method. Result: Phenolic compounds are responsible for antioxidant activity. Plant extracts having hydroxyl groups are responsible for facilitating free radical scavenging. Total phenol content in the plant extract Curcuma longa and Camellia sinensisusing the calibration curve, was found to be 1137.45mg of Gallic acid equivalents/g dry weight of extract. The anticancer activity was carried out with lung cancer cell line (A-549) by MTT assay method. When cancer cells were incubated with combination of curcuma longa and camellia sinensis extracts, they induced cytotoxicity in a significant time which implicit the damage to the membrane integrity of cell. Based on the cell disintegration, when the plant extracts were incubated with lung cancer cells and cell death were calculated. The IC50 value of the given test sample of curcuma longa and camellia sinensisand the standard Cisplatin was found to be 22.72µg and 3.89µg, respectively. This is clearly stated that the selection of A-549 cell line might provide a good evidence for the treatment of lung cancer. Conclusion: Based on the results obtained, combinations of Curcuma longa and Camellia sinensis could be used as an anticancer agent.

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Phytochemical analysis and assessment of Antioxidant properties of black tea extract obtained from Camellia sinensis

Cited by 5 publications

References 0 publications

Detection of Antimicrobial, Antioxidant and Cytotoxicity Activities of Fusarium oxysporum F01 Isolated from Catharanthus roseus Collected in Vietnam

Detection of Antimicrobial, Antioxidant and Cytotoxicity Activities of Fusarium oxysporum F01 Isolated from Catharanthus roseus Collected in Vietnam

Evaluation of Sertraline as an antioxidant, anti-inflammatory and anticataract

Anticancer Activity of Microwave Assisted Polyphenolic Compounds Extracted from Combinations of Curcuma Longa and Camellia Sinensis against Lung Cancer Cell Line

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