Phytochemical analysis and antioxidant capacity ofTabernaemontana catharinensis A. DC. Fruits and branches

Abstract

The antioxidant capacity of the crude extract and fractions of Tabernaemontana catharinensis fruits and branches, was evaluated by the 2,2-diphenyl-1-picrylhydrazyl (DPPH) method and the content of polyphenols, flavonoids, alkaloids and condensed tannins were determined by the spectrophotometric method. The ethyl acetate fraction of the fruits and the n-butanol fraction of the branches showed IC 50 of 181.82 µg/mL and 78.19 µg/mL, respectively. All fractions were analyzed by high performance liquid chromatography (HPLC), in the branches were quantified chlorogenic acid in the chloroform (8.96 mg/g), ethyl acetate (4.31 mg/g) and n-butanol (3.33 mg/g) fractions; caffeic acid in the ethyl acetate (5.24 mg/g) and n-butanol (1.81 mg/g); gallic acid (0.52 mg/g) in the n-butanol. In the fruits, chlorogenic acid in the chloroform (1.67 mg/g); rutin in the ethyl acetate (3.45 mg/g) and n-butanol (8.98 mg/g) fractions.The present study showed that these quantified compounds can contribute to antioxidant capacity which was higher in the branches than in the fruits.

Phenolic Acid Profiling in the Leaves of Tabernaemontana Heyneana Wall. An Endemic Plant of the Western Ghats Using Ultra-High Performance Liquid Chromatography Coupled With Quadrupole-Time-of-Flight

“…In the present study, cinnamic acid derivatives were detected in both the modes of ionization which is in agreement with the previously published data [17]. Chlorogenic acid was earlier reported in Tabernaemontana catharinensis by Piana et al [13] and ferulic acid in Tabernaemontana coronaria by El-Gayed et al [14]. Sinapyl alcohol was documented earlier in cell suspension cultures of Tabernaemontana divaricata by Dagnino et al [18].…”

“…However, the positive ionization [M+H] + mode may reveal more structural and fragmentation information about the flavonoid. Plenty of flavonoids synthesized in plants is linked to sugars (glycosides), despite being Previous studies reported the presence of rutin in T. heyneana [8] and Tabernaemontana catharinensis [13]. El-Gayed et al [14] also witnessed the identification of hesperidin in Tabernaemontana coronaria.…”

Identification of Alkaloid’s Profile in Ficus benjamina L. Extracts with Higher Antioxidant Power

“…DPPH assay results show that F. benjamina crude extracts presents a high antioxidant capacity, when compared with literature. In fact, a previous paper [29] reported that crude extracts of Tabernaemontana catharinensis fruits and branches, evaluated by the DPPH method and the content of alkaloids, respectively show an IC 50 of 1155.91 mg·ml −1 of fruits crude extract and 202.17 mg·ml −1 of branches crude extract. However, T. catharinensis contained more alkaloids equivalent with respect to F. benjamina.…”

Neoformação in vitro de Parte Aérea e Enraizamento Adventício de Brotações de Tabernaemontana catharinensis A. DC

“…(Apocynaceae), nativa do Rio Grande do Sul (PEREIRA et al, 2008), popularmente conhecida como "cobrina" ou "jasmim-cata-vento" (LORENZI, 2009) demonstrou, em estudos fitoquímicos, ser rica em flavonoides, saponinas, terpenos, alcaloides e esteroides (BÉLO et al, 2009). Seus ramos possuem atividade antioxidante, revelando além da rutina, um glicosídeo de quercetina e ácido clorogênico (PIANA et al, 2014). O chá ou a infusão das folhas e cascas são utilizados na medicina popular brasileira como antídoto para picadas de cobra, para aliviar dor de dente, tratamento de feridas, herpes, tumores e, ainda, como hemostática, hipotensora e cardiotônica (PEREIRA et al, 2005).…”