Phytoalexin Accumulation in Colombian Bean Varieties and Aminosugars as Elicitors

Durango, Diego; Quiñones, Winston; Torres, Fernando; Rosero, Yoni; Gil, José; Echeverri, Fernándo

doi:10.3390/71100817

Cited by 50 publications

(39 citation statements)

References 17 publications

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“…Plants respond to attack of pathogens, insects and hervibores or to other biotic and abiotic stresses by activating an array of defense mechanisms including induction of biosynthesis of secondary metabolites as phytoalexins, hypersensitive responses and structural defensive barriers, such as lignin deposition on cell wall, among others [5][6][7][8].…”

Section: Plant Secondary Metabolism and Defense Response: Elicitor Comentioning

confidence: 99%

Molecular aspects of the early stages of elicitation of secondary metabolites in plants

2007

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Section: Plant Secondary Metabolism and Defense Response: Elicitor Comentioning

confidence: 99%

Molecular aspects of the early stages of elicitation of secondary metabolites in plants

2007

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“…These compounds have an important role in normal development and can serve as protection (phytoalexins) against many environmental sources of stress such as nutrient deficiency, prolonged cold, pathogen attack, and exposure to UV light [13,14]. Till date, UV treatment or chemical elicitation on legume plants were investigated to improve the knowledge on the biochemical bases of plant defense against biotic and abiotic stress [13,15,16].…”

Section: Introductionmentioning

confidence: 99%

Compositional changes induced by UV‐B radiation treatment of common bean and soybean seedlings monitored by capillary electrophoresis with diode array detection

Dinelli¹,

Aloisio²,

Bonetti³

et al. 2007

J of Separation Science

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In this work, a new CE method with diode array detection (DAD) was developed for the monitoring and quantitation of flavonoids in different beans treated and untreated with UV-B radiation. Flavonoid concentration was monitored in UV-B-treated and untreated sprouts of three common beans (Zolfino ecotype, cv. Verdone, cv. Lingua di Fuoco) and one soybean (cv. Pacific). After acid hydrolysis of extracts, the CE-DAD method provides reproducible quantitative determinations of daidzein, glycitein, genistein, and kaempferol at ppm level in these natural matrices within a relatively short time (less than 16 min). Total flavonoid content determined by CE-DAD was 159 +/- 8, 26 +/- 2, 13 +/- 1, and 1.3 +/- 0.3 microg/g fresh weight for untreated sprouts of Pacific soybean, Verdone bean, Zolfino bean, and Lingua di Fuoco bean, respectively. UV-B treatment caused no significant quantitative effect on Pacific soybean sprouts, whereas it enhanced the total isoflavone content by 1.5, 1.8, and 3.2-fold in Verdone, Zolfino, and Lingua di Fuoco beans, respectively. The proposed method shows (i) the potentialities of bean sprouts as a natural source of bioactive compounds (antioxidants); (ii) the technological role of UV-B treatment for sprout isoflavone enrichment; and (iii) the good capabilities of CE-DAD to monitor this process.

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“…This is in agreement with findings for phytoalexins accumulation in beans infected with Colletotrichum lindemuthianum (Sacc. & Magnus) Briosi & Cavara [30]. This suggests that in cultivar Meli cyclization of daidzein is favoured and results in production of pterocarpans and coumestans.…”

Section: Discussionmentioning

confidence: 92%

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

et al. 2013

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Soybean [Glycine max (L.) Merr.] cultivars (Meli, Alisa, Sava and 1511/99) were grown up to V1 phase (first trifoliate and one node above unifoliate) and then inoculated with Sclerotinia sclerotiorum (Lib.) de Bary under controlled conditions. Changes in L-phenylalanine ammonia-lyase (PAL) activity and isoflavone phytoalexins were recorded 12, 24, 48 and 72 h after the inoculation. Results showed an increase in PAL activity in all four examined soybean cultivars 48 h after the inoculation, being the highest in Alisa (2-fold higher). Different contents of total daidzein, genistein, glycitein and coumestrol were detected in all samples. Alisa and Sava increased their total isoflavone content (33.9% and 6.2% higher than control, respectively) as well as 1511/99, although 48 h after the inoculation its content decreased significantly. Meli exhibited the highest rate of coumestrol biosynthesis (72 h after the inoculation) and PAL activity (48 h after the inoculation). All investigated cultivars are invariably susceptible to this pathogen. Recorded changes could point to possible differences in mechanisms of tolerance among them.

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Phytoalexin Accumulation in Colombian Bean Varieties and Aminosugars as Elicitors

Cited by 50 publications

References 17 publications

Molecular aspects of the early stages of elicitation of secondary metabolites in plants

Molecular aspects of the early stages of elicitation of secondary metabolites in plants

Compositional changes induced by UV‐B radiation treatment of common bean and soybean seedlings monitored by capillary electrophoresis with diode array detection

Changes in L-phenylalanine ammonia-lyase activity and isoflavone phytoalexins accumulation in soybean seedlings infected with Sclerotinia sclerotiorum

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