Phyto-synthesis of silver nanoparticles using Alternanthera tenella leaf extract: an effective inhibitor for the migration of human breast adenocarcinoma (MCF-7) cells

Abstract

In this study, phyto-synthesis of silver nanoparticles (AgNPs) was achieved using an aqueous leaf extract of Alternanthera tenella. The phytochemical screening results revealed that flavonoids are responsible for the AgNPs formation. The AgNPs were characterised using UV-visible spectrophotometer, field emission scanning microscopy/energy dispersive X-ray, transmission electron microscopy, fourier transform infrared spectroscopy (FT-IR), and X-ray diffraction. The average size of the nanoparticles was found to be ≈48 nm. The EDX results show that strong signals were observed for the silver atoms. The strong band appearing at 1601-1595 cm(-1) correspond to C-C stretching vibration from dienes in FT-IR spectrum indicating the formation of AgNPs. Human breast adenocarcinoma (MCF-7) cells treated with various concentrations of AgNPs showed a dose-dependent increase in cell inhibition. The IC50 value of the AgNPs was calculated to be 42.5 μg mL(-1). The AgNPs showed a significant reduction in the migration of MCF-7 cells.

Rapid Biosynthesis of Silver Nanoparticles Using Pepino (Solanum muricatum) Leaf Extract and Their Cytotoxicity on HeLa Cells

“…However, some reports on the modified use of plant extracts have given insight into the possible organic moieties responsible for the metal ion reduction process along with greatly reducing the time duration for the synthesis [30,31,32,33]. The core-shell is nevertheless maintained.…”

“…Recently, MNPs have gained special attention for their promising potential as anticancer agent [12,13,31,36,39,40,41,42]. Characteristics as their intrinsic cytotoxicity, their larger surface area, and area:volume ratio, their easily tunable surface that allow conjugation or encapsulation with biological targeting or therapeutic molecules, and their optical characteristics, make them optimal materials for cancer theranostics [39,43] which try to combine diagnosis and therapy into a single agent.…”

Green Synthesis of Plant-Mediated Metal Nanoparticles: The Role of Polyphenols

“…At present, limited information is available in the scientific literature regarding the use of a single substance from plant extract for the synthesis of MNPs. Recent reports in literature on this issue show that the flavonoids which have a wide existence in the plant extracts have a major contribution toward the bioreduction, capping, and stabilization of metal ions into NPs formation [28][29][30][31].…”

“…The detail of polyphenols and flavonoids employed in the synthesis of MNPs along with their biomedical efficacy is summarized in Table 2. Recently, it was reported that the major contribution for the synthesis of silver NPs (AgNPs) was of the total flavonoids present in the Alternanthera tenella and Coriandrum sativum leaf extracts [7]; and shown to be efficacious as antiacne, antidandruff, and anti-breast cancer agent as they were found active against Propionibacterium acnes, Malassezia furfur, and human breast adenocarcinoma cells, respectively [28,29].…”

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“…Green nanotechnology or green synthesis is nothing but an organic synthesis of nanoparticles using plant extracts and the synthesized nanoparticles are then known as biogenic nanoparticles. In numerous studies huge number of medicinal plants are used to synthesize the silver NPs 74, 75 like Mulberry leaves 76 , Alternanthera dentate 77 , Ocimum sanctum 78 , Azadirachta indica 79 , Brassica rapa, Coccinia indica, Vitex negundo, Melia dubia are used have already been used to synthesize and stabilize metallic nanoparticles, very particularly biogenic silver (Ag) nanoparticles.…”