Phyto- and Bio-Chemical evaluation of Diospyros kaki L. cultivated in Egypt and its biological activities

El‐Hawary, Seham S.; Tadros, Soad H.; Abdel-Mohsen, Mona Mohamed; Mohamed, Magda S.; Sheikh, E. El; Nazif, Naglaa M.

doi:10.1590/1519-6984.200460

Cited by 6 publications

(4 citation statements)

References 36 publications

(35 reference statements)

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“…On the other hand, the methanolic extract of D. villosa plant showed the lowest potency in inhibiting cell growth in MCF-7 and A549 cells. Taking in account that the beneficial properties of Diospyros plants are related to a variety of bioactive components that enhance antioxidant capacity and consequently anticancer activity [ 33 , 34 ]. Diospyros villosa may be strong candidates for future cancer studies, having high antioxidant and anticancer activity.…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic Potential of Diospyros villosa Leaves and Stem Bark Extracts and Their Silver Nanoparticles

Adu

Naidoo

Lin

et al. 2023

Plants

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Diospyros villosa is traditionally used for an anti-bacterial property. Its cytotoxic effects have not been studied before. Therefore, this study aimed to examine the nutritional properties as well the cytotoxic effects of D. villosa. The leaves and stem barks were subjected to three different extraction methods (methanol, chloroform and hexane) and their nanoparticles were synthesized at two different temperatures (room temperature and at 80 °C). Thereafter, extracts were assessed using the associated AOCC protocols, for their nutritional content (moisture, fibre, proteins, lipid, ash and hydrolysable carbohydrates). Diospyros villosa extracts and their corresponding nanoparticles were then incubated overnight with cancerous and noncancerous cell lines to evaluate their cytotoxic potential. The nutritional analysis revealed that both young and mature leaves were rich sources of protein having values of 14.95% and 11.37% respectively. The moisture content was observed to be higher in all the leaf types (8.54 ± 0.75%, 9.67 ± 0.98% and 7.40 ± 0.80%) compared to the stem (2.13 ± 0.07%) respectively. The MTT cytotoxicity assay showed that the cell viability of MCF-7 cell lines was significantly lower when exposed to hexane and chloroform leaves extracts of D. villosa (IC50 of 26.64 and 26.07 µg mL−1) respectively, compared to camptothecin (36.54 µg mL−1). Similarly, the MCF-7 cell viability was observed to be significantly lower when exposed to hexane and chloroform stem extracts of D. villosa (IC50 of 24.57 and 3.92 µg mL−1), compared to camptothecin (36.54 µg mL−1). The cell viability of A549 cell lines was also found lower when exposed to the hexane and chloroform extracts (IC50 of 7.76 and 4.59 µg mL−1) compared to camptothecin (IC50 of 19.26 µg mL−1). Furthermore, the viability of A549 cell lines was found lower when exposed to hexane and chloroform stem extracts of D. villosa (IC50 of 10.67 and 5.35 µg mL−1) compared to camptothecin (19.26 µg mL−1). The biosynthesized nanoparticles further displayed an anticancer activity with an IC50 value of 4.08 µg mL−1 when compared to the control (36.54 µg mL−1). However, the HEK293 cell viability was observed to be significantly higher on exposure to hexane stem extracts of D. villosa (IC50 of 158.5 µg mL−1) compared to camptothecin (IC50 of 14.77 µg mL−1). Therefore, Diospyros villosa leaves, stem bark and nanoparticles synthesized showed high potential for being considered as a candidate for an anti-cancer regimen.

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Section: Discussionmentioning

confidence: 99%

Cytotoxic Potential of Diospyros villosa Leaves and Stem Bark Extracts and Their Silver Nanoparticles

Adu

Naidoo

Lin

et al. 2023

Plants

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“…The major saturated fatty acid methyl ester was methyl palmitate, whereas the major unsaturated fatty acid methyl ester was methyl 9-octadecenoate (El-Hawary et al, 2020).…”

Section: Miscellaneous Compoundsmentioning

confidence: 95%

“…Moreover, GC–MS analysis of the fatty acids methyl esters of leaves revealed the presence of 10 fatty acids. The major saturated fatty acid methyl ester was methyl palmitate, whereas the major unsaturated fatty acid methyl ester was methyl 9‐octadecenoate (El‐Hawary et al, 2020).…”

Section: Chemical Diversity Of Dsmentioning

confidence: 99%

Unveiling major ethnopharmacological aspects of genus Diospyros in context to its chemical diversity: A comprehensive overview

Fareed

El‐Kersh

Youssef

et al. 2022

Journal of Food Biochemistry

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Diospyros species (DS), “Ebenaceae,” were known for their therapeutic uses in folk medicine since days of yore. Thereafter, scientific evidence related their health benefits to a myriad of chemical classes, for instance, naphthoquinones, flavonoids, tannins, coumarins, norbergenin derivatives, sterols, secoiridoids, sesquiterpenes, diterpenoids, triterpenoids, volatile organic compounds (VOCs), and carotenoids. The available literature showed that more than 200 compounds were isolated and identified via spectroscopic techniques. Many pharmacological activities of DS have been previously described, such as antioxidant, neuroprotective, antibacterial, antiviral, antiprotozoal, antifungal, antiinflammatory, analgesic, antipyretic and cosmeceutical, investigated, and confirmed through versatile in vitro and in vivo assays. Previous studies proved that genus Diospyros is a rich reservoir of valuable bioactive compounds. However, further comparative studies among its different species are recommended for more precise natural source‐based drug discovery and clinical application. Accordingly, this review is to recall the chemical abundance and diversity among different members of genus Diospyros and their ethnopharmacological and pharmacological uses. Practical Applications Practically, providing sufficient background on both secondary metabolites divergence and pharmacological properties of genus Diospyros has many fruitful aspects. As demonstrated below, extracts and many isolated compounds have significant curative properties, which can lead to the discovery of pharmaceutically relevant alternative substitutes to conventional medicine. Consequently, molecular docking on various receptors can be applied. On the grounds, Naoxinqing tablets, a standardized herbal product containing D. kaki leaves extract, have been patented and recorded in Chinese Pharmacopeia as an approved Traditional Chinese Medicine (TCM) for the treatment of cerebro‐ and cardiovascular diseases, although the underlying mechanism remains under advisement. Moreover, the antimicrobial applications of DS are of considerable concern; since the widespread use of antibiotics resulted in different forms of bacterial resistance, hence, limiting and compromising effective treatment. In addition, as a result of contemporary rampant memory disorders, neuroprotective activities of different extracts of DS became of great emphasis.

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“…ability to scavenge free radicals (Nichols and Katiyar, 2010;Souza et al, 2018;Hollman and Katan, 1999). In addition, these compounds also present antibacterial, antiviral, anti-inflammatory and anti-allergic action, as well as photoprotective activities (Marwah et al, 2007;Cook and Samman, 1996;El-Hawary et al, 2020). Thus, flavonoid-rich extracts stand out as a viable option for the development of pharmaceutical products, particularly sunscreens.…”

Section: Fractionation Of the Extractmentioning

confidence: 99%

Evaluation of antioxidant, photoprotective and antinociceptive activities of Marcetia macrophylla extract: potential for formulation of sunscreens

et al. 2023

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The antioxidant, photoprotective and antinociceptive Marcetia macrophylla active extract was investigated as an active ingredient in a sunscreen cream formulation. Thus, the M. macrophylla extract showed IC50 of 3.43 mg/ml of the antioxidant (DPPH∙ scavenging test) and Sun Protection Factor of 20.25 (SPF/UV-B, at 250 µg/ml) and UV-A of 78.09% (photobleaching trans-resveratrol test). The antinociceptive activity was superior to all standards tested using the in vivo acetic acid-induced writhing test (99.14% at the dose of 200 mg/kg) and the high-performance liquid chromatography coupled with diode array detector and mass spectroscopy multi-stage (HPLC-DAD-MS/MS) enabled the structural characterization of the quercetin-3-O-hexoside, quercetin-3-O-pentoside and quercetin-3-O-desoxihexoside. The pharmaceutical formulation containing the Marcetia macrophylla crude active extract was prepared and the physicochemical tests (organoleptic characteristics, pH analysis and centrifugation), the in vitro UVB (sun protection factor, SPF) and UVA (β-carotene) using the spectroscopic method were investigated. The formulation showed satisfactory results concerning the physicochemical parameters evaluated and active against the UV test. Thus, M. macrophylla showed biological activities with potential use in pharmaceutical preparations.

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Phyto- and Bio-Chemical evaluation of Diospyros kaki L. cultivated in Egypt and its biological activities

Cited by 6 publications

References 36 publications

Cytotoxic Potential of Diospyros villosa Leaves and Stem Bark Extracts and Their Silver Nanoparticles

Cytotoxic Potential of Diospyros villosa Leaves and Stem Bark Extracts and Their Silver Nanoparticles

Unveiling major ethnopharmacological aspects of genus Diospyros in context to its chemical diversity: A comprehensive overview

Evaluation of antioxidant, photoprotective and antinociceptive activities of Marcetia macrophylla extract: potential for formulation of sunscreens

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