Phytic acid and raffinose series oligosaccharides metabolism in developing chickpea seeds

Abstract

Phytic acid and raffinose series oligosaccharides (RFOs) have anti-nutritional properties where phytic acid chelates minerals and reduces their bioavailability to humans and other animals, and RFOs cause flatulence. Both phytic acid and RFOs cannot be digested by monogastric animals and are released as pollutant-wastes. Efforts are being made to reduce the contents of these factors without affecting the viability of seeds. This will require a thorough understanding of their metabolism in different crops. Biosynthetic pathways of both metabolites though are interlinked but not well described. This study was made on metabolism of these two contents in developing chickpea (Cicer arietinum L cv GL 769) seeds. In this study, deposition of RFOs was found to occur before deposition of phytic acid. A decline in inorganic phosphorus and increase in phospholipid phosphorus and phytic acid was observed in seeds during development. Acid phosphatase was the major phosphatase in seed as well as podwall and its activity was highest at early stage of development, thereafter it decreased. Partitioning of 14 C label from 14 Cglucose and 14 C-sucrose into RFOs and phytic acid was studied in seeds in presence of inositol, galactose and iositol and galactose, which favored the view that galactinol synthase is not the key enzyme in RFOs synthesis.

Natural Variation for Seed Physical, Biochemical and Culinary traits in Common bean (Phaseolus vulgaris L.)

“…In fact, the mutations in the MIPS enzyme have been reported to decrease the both in soybean (Hitz et al, 2011). Similar results have been reported by Zhawar et al, (2011) in chickpea. Phytic acid was also significantly and positively correlated with protein.…”

Spectrophotometric Quantification of Phytic Acid During Embryogenesis in Bambara groundnut (Vigna subterranea L.) Through Phosphomolybdenum Complex Formation

“…Based on the strong correlation between phytic acid and phosphorus in chickpea (Cicer arietinum) (Zhawar et al, 2011), soybean (Glycine max) (Zhang et al, 2007), common bean (Phaseolus vulgaris L.) (Walker, 1974) and cereals (Doria et al, 2009;Jennings and Morton, 1963;Karner et al, 2004;Williams, 1970), we partially conclude that G340A had the highest concentration of phytic acid. Phytic acid has implications in imparting drought tolerance and seed desiccation tolerance.…”

Effects of Heat Stress on Metabolite Accumulation and Composition, and Nutritional Properties of Durum Wheat Grain

“…In plants, elevated levels of polyamines are one of the most remarkable changes that occur in response to abiotic stress conditions [ 70 , 108 ]; however, in response to heat stress, there was only an increase for “Primadur”. Raffinose also increased in response to the heat stress for “Primadur”, and it is known to have antioxidant and anti-nutritional properties because it cannot be digested by monogastric animals [ 109 , 110 ]. Therefore, our data indicate that heat stress can affect the accumulation of these compounds in different ways, which can result in either beneficial or detrimental effects on human health in relation to the wheat genotype and the environmental conditions that occur during the plant growth.…”