Physiology of the Silkworm. I. Growth and Respiration of Bombyx Mori during Its Entire Life-Cycle

Hsueh, Tun-Yun; Tang, Peng

doi:10.1086/physzool.17.1.30151830

Cited by 13 publications

(4 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous work on allometric scaling in B. mori has shown that mass-specific metabolic rate is lowest during the pupal stage, but the age of pupae used in that study was not reported (Blossman-Myer and Burggren, 2010a). Here, pupae were 8-12 days old, which is likely the nadir of the U-shaped curve of responses (Odell, 1998) because pupation in B. mori usually lasts 14-21 days (Hsueh and Tang, 1944). While this would require further investigation, the only proxy we have for age would be mass because pupal mass decreases from completion of the cocoon to time of emergence (Hsueh and Tang, 1944).…”

Section: Fig 3 Examples Of Thermolimit Respirometry Recordings Of Bmentioning

confidence: 98%

“…Here, pupae were 8-12 days old, which is likely the nadir of the U-shaped curve of responses (Odell, 1998) because pupation in B. mori usually lasts 14-21 days (Hsueh and Tang, 1944). While this would require further investigation, the only proxy we have for age would be mass because pupal mass decreases from completion of the cocoon to time of emergence (Hsueh and Tang, 1944). As mass is not significantly different between pupae treated with normoxia and hypoxia (MannWhitney U, Z=0.65, P=0.65), and pupae were assigned at random to the gas treatments, age is unlikely to have significantly biased the outcomes of our study.…”

Section: Fig 3 Examples Of Thermolimit Respirometry Recordings Of Bmentioning

confidence: 98%

See 1 more Smart Citation

Oxygen safety margins set thermal limits in an insect model system

Boardman

Terblanche

2015

Journal of Experimental Biology

View full text Add to dashboard Cite

A mismatch between oxygen availability and metabolic demand may constrain thermal tolerance. While considerable support for this idea has been found in marine organisms, results from insects are equivocal and raise the possibility that mode of gas exchange, oxygen safety margins and the physico-chemical properties of the gas medium influence heat tolerance estimates. Here, we examined critical thermal maximum (CT max ) and aerobic scope under altered oxygen supply and in two life stages that varied in metabolic demand in Bombyx mori (Lepidoptera: Bombycidae). We also systematically examined the influence of changes in gas properties on CT max . Larvae have a lower oxygen safety margin (higher critical oxygen partial pressure at which metabolism is suppressed relative to metabolic demand) and significantly higher CT max under normoxia than pupae (53°C vs 50°C). Larvae, but not pupae, were oxygen limited with hypoxia (2.5 kPa) decreasing CT max significantly from 53 to 51°C. Humidifying hypoxic air relieved the oxygen limitation effect on CT max in larvae, whereas variation in other gas properties did not affect CT max . Our data suggest that oxygen safety margins set thermal limits in air-breathing invertebrates and the magnitude of this effect potentially reconciles differences in oxygen limitation effects on thermal tolerance found among diverse taxa to date.

show abstract

Section: Fig 3 Examples Of Thermolimit Respirometry Recordings Of Bmentioning

confidence: 98%

Section: Fig 3 Examples Of Thermolimit Respirometry Recordings Of Bmentioning

confidence: 98%

Oxygen safety margins set thermal limits in an insect model system

Boardman

Terblanche

2015

Journal of Experimental Biology

View full text Add to dashboard Cite

show abstract

“…This is a common challenge for translating alternative silk-producing species to biomedical applications, and while Plodia ’s fibroin proteins do not contain RGD, its standardized indoor rearing parameters warrant further investigation on how its unique sequence in the silk biomaterial space can be leveraged. An additional advantage is that Plodia can produce collectable silk fibers in a shorter life cycle compared to many other silkworms, such as Bombyx or Antheraea species. , The life cycle length for Bombyx ranges between 45 and 55 days, while the Plodia life cycle length ranges from 20 to 35 days (temperature-dependent). Antheraea mylitta is also longer, ranging from 35 to 45 days .…”

Section: Introductionmentioning

confidence: 99%

“…An additional advantage is that Plodia can produce collectable silk fibers in a shorter life cycle compared to many other silkworms, such as Bombyx or Antheraea species. 32,33 The life cycle length for Bombyx ranges between 45 and 55 days, 34 while the Plodia life cycle length ranges from 20 to 35 days (temperature-dependent). Antheraea mylitta is also longer, ranging from 35 to 45 days.…”

Section: Introductionmentioning

confidence: 99%

Harvesting Silk Fibers from Plodia interpunctella: Role of Environmental Rearing Conditions in Fiber Production and Properties

Shirk,

Torres Pereira Meriade Duarte,

McTyer

et al. 2024

ACS Biomater. Sci. Eng.

View full text Add to dashboard Cite

Silk fibers are produced by a wide variety of insects. The silkworm Bombyx mori (Bombyx) was domesticated because the physical properties of its silk fibers were amenable to the production of fine textiles. Subsequently, engineers have regenerated silk fibroin to form biomaterials. The monocular focus on Bombyx silk has underutilized the expanse of diverse silk proteins produced by more than 100,000 other arthropods. This vast array of silk fibers could be utilized for biomedical engineering challenges if sufficient rearing and purification processes are developed. Herein, we show that the moth, Plodia interpunctella (Plodia), represents an alternative silk source that is easily reared in highly regulated culture environments allowing for greater consistency in the silk produced. We controlled the temperature, resource availability (larvae/gram diet), and population density (larvae/mL) with the goal of increasing silk fiber production and improving homogeneity in Plodia silk proteins. We determined that higher temperatures accelerated insect growth and reduced life cycle length. Furthermore, we established initial protocols for the production of Plodia silk with optimal silk production occurring at 24 °C, with a resource availability of 10 larvae/gram and a population density of 0.72 larvae/mL. Population density was shown to be the most prominent driving force of Plodia silk mat formation among the three parameters assessed. Future work will need to link gene expression, protein production and purification, and resulting mechanical properties as a function of environmental cues to further transition Plodia silk into regenerated silk fibroin biomaterials.

show abstract

Empirical formulae and the surface area of puparia of Drosophila melanogaster, Meigen

Ellenby

1945

J. Exp. Zool.

View full text Add to dashboard Cite

Physiology of the Silkworm. I. Growth and Respiration of Bombyx Mori during Its Entire Life-Cycle

Cited by 13 publications

References 0 publications

Oxygen safety margins set thermal limits in an insect model system

Oxygen safety margins set thermal limits in an insect model system

Harvesting Silk Fibers from Plodia interpunctella: Role of Environmental Rearing Conditions in Fiber Production and Properties

Empirical formulae and the surface area of puparia of Drosophila melanogaster, Meigen

Contact Info

Product

Resources

About