Physiology Of Root-Nodule Bacteria

Poole, Philip S.; Hynes, Michael F.; Johnston, Andrew; Tiwari, Ravi; Reeve, W.G.; Downie, J. A.

doi:10.1007/978-1-4020-3548-7_9

Cited by 4 publications

(3 citation statements)

References 323 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Considerable efforts have been devoted to sourcing strains from different geographical locations in order to improve legume productivity across a range of environments, and the project takes advantage of the particularly well characterized RNB that have been sourced from several culture collections around the globe. Biogeographic considerations are particularly relevant to the RNB as their survival and persistence as soil saprophytes is dictated by environmental and edaphic constraints such as temperature, salinity, pH, and soil moisture and clay content [ 28 ].…”

Section: The Scientific Questions We Expect To Answermentioning

confidence: 99%

A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey

Reeve

Ardley

Tian

et al. 2015

Stand in Genomic Sci

View full text Add to dashboard Cite

Root nodule bacteria are free-living soil bacteria, belonging to diverse genera within the Alphaproteobacteria and Betaproteobacteria, that have the capacity to form nitrogen-fixing symbioses with legumes. The symbiosis is specific and is governed by signaling molecules produced from both host and bacteria. Sequencing of several model RNB genomes has provided valuable insights into the genetic basis of symbiosis. However, the small number of sequenced RNB genomes available does not currently reflect the phylogenetic diversity of RNB, or the variety of mechanisms that lead to symbiosis in different legume hosts. This prevents a broad understanding of symbiotic interactions and the factors that govern the biogeography of host-microbe symbioses.Here, we outline a proposal to expand the number of sequenced RNB strains, which aims to capture this phylogenetic and biogeographic diversity. Through the Vavilov centers of diversity (Proposal ID: 231) and GEBA-RNB (Proposal ID: 882) projects we will sequence 107 RNB strains, isolated from diverse legume hosts in various geographic locations around the world. The nominated strains belong to nine of the 16 currently validly described RNB genera. They include 13 type strains, as well as elite inoculant strains of high commercial importance. These projects will strongly support systematic sequence-based studies of RNB and contribute to our understanding of the effects of biogeography on the evolution of different species of RNB, as well as the mechanisms that determine the specificity and effectiveness of nodulation and symbiotic nitrogen fixation by RNB with diverse legume hosts.

show abstract

Section: The Scientific Questions We Expect To Answermentioning

confidence: 99%

A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey

Reeve

Ardley

Tian

et al. 2015

Stand in Genomic Sci

View full text Add to dashboard Cite

show abstract

“…The genome of this strain does contain orthologs of acid response or acid tolerance genes that were initially discovered in E. medicae WSM419 . These genes include actA ( lnt ), actP , actR , actS , phrR , lpiA and acvB [ 32 – 35 ]. WSM244 also contains the tcsA - tcrA - fsrR - regulatory gene cluster which is required for the low-pH-activation of lpiA and acvB in E. medicae WSM419 [ 36 ].…”

Section: Insights From the Genome Sequencementioning

confidence: 99%

High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil

Ardley

Tian

O’Hara

et al. 2015

Stand in Genomic Sci

View full text Add to dashboard Cite

Ensifer medicae WSM244 is an aerobic, motile, Gram-negative, non-spore-forming rod that can exist as a soil saprophyte or as a legume microsymbiont of Medicago species. WSM244 was isolated in 1979 from a nodule recovered from the roots of the annual Medicago polymorpha L. growing in alkaline soil (pH 8.0) in Tel Afer, Iraq. WSM244 is the only acid-sensitive E. medicae strain that has been sequenced to date. It is effective at fixing nitrogen with M. polymorpha L., as well as with more alkaline-adapted Medicago spp. such as M. littoralis Loisel., M. scutellata (L.) Mill., M. tornata (L.) Mill. and M. truncatula Gaertn. This strain is also effective with the perennial M. sativa L. Here we describe the features of E. medicae WSM244, together with genome sequence information and its annotation. The 6,650,282 bp high-quality permanent draft genome is arranged into 91 scaffolds of 91 contigs containing 6,427 protein-coding genes and 68 RNA-only encoding genes, and is one of the rhizobial genomes sequenced as part of the DOE Joint Genome Institute 2010 Genomic Encyclopedia for Bacteria and Archaea-Root Nodule Bacteria (GEBA-RNB) project proposal.Electronic supplementary materialThe online version of this article (doi:10.1186/s40793-015-0119-5) contains supplementary material, which is available to authorized users.

show abstract

“…They are truly characterized by their capacity to form nodules on the roots and sometimes the stems of host plants generally legumes and to grow on the isolation YEM medium (Vincent, 1970). Inside the nodule and in the bacteroids form and via the nitrogenase enzyme activity, they fix and reduce atmospheric nitrogen (N 2 ) to ammonia (NH 3 ) exploitable by the host plant (Dixon and Wheeler, 1986;Graham, 2008). Thus, these symbionts are widely used in the International Journal of Current Microbiology and Applied Sciences…”

Section: Introductionmentioning

confidence: 99%

Molecular and Symbiotic Efficiency Characterization of Rhizobia Nodulating Bambara Groundnut (Vigna subterranea L.) from Agricultural Soils of Daloa Localities in Côte D’ivoire

Raissa¹,

Konaté²,

Taha³

et al. 2020

Int.J.Curr.Microbiol.App.Sci

View full text Add to dashboard Cite

Physiology Of Root-Nodule Bacteria

Cited by 4 publications

References 323 publications

A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey

A Genomic Encyclopedia of the Root Nodule Bacteria: assessing genetic diversity through a systematic biogeographic survey

High-quality permanent draft genome sequence of Ensifer medicae strain WSM244, a microsymbiont isolated from Medicago polymorpha growing in alkaline soil

Molecular and Symbiotic Efficiency Characterization of Rhizobia Nodulating Bambara Groundnut (Vigna subterranea L.) from Agricultural Soils of Daloa Localities in Côte D’ivoire

Contact Info

Product

Resources

About