Molecular and Symbiotic Efficiency Characterization of Rhizobia Nodulating Bambara Groundnut (Vigna subterranea L.)  from Agricultural Soils of Daloa Localities in Côte D’ivoire

Raissa, Guei Nassé Kaéda; Konaté, Ibrahim; Taha, Kaoutar; Bamba, Issouf; Maxwell, Beugre Grah Avit; Sélastique, Akaffou Doffou; Abdelkarim, Filali Maltouf

doi:10.20546/ijcmas.2020.907.056

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…The PCR mixture was prepared following the 2X GoTaq PCR mix (Promega, Wisconsin, USA) protocol with universal bacterial primers 27F and 1492R (Table 3). Running PCR accomplished using a thermocycler following the amplified condition by Raissa et al [30]. Furthermore, the PCR results were visualized on agarose gel and observed using UV through the GelDoc (UVITEC, England, UK) System.…”

Section: S Rrna Molecular Identification Of Rhizobia Isolatementioning

confidence: 99%

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Wulandari,

Baskoro,

Mahmuudah

et al. 2024

Trends Sci

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Rhizobia are bacteria that symbiosis with host plant, as shown in the root nodules formation, and provide nitrogen that can be absorbed by plants in greater quantities than rhizobacteria. Available Nitrogen, which absorbed by plants, is the essential requirement for plant growth because its role in increasing yield and quality, hence it is needed in greater quantities than other nutrients. The study aimed to determine the macroscopic and microscopic diversity of rhizobia isolates from the groundnut nodules and their potential as PGPRs, and to identify 16S rRNA isolates with the best potential as PGPRs molecularly. The methods used were isolation from root nodules, screening of PGPR potential, molecular identification based on the 16S rRNA gene, and phylogenetic analysis to determine their kinship. Based on the isolation results, 17 Gram-negative isolates were obtained white to pink or orange color on AG media with various colony characteristics in terms of shape, margin, elevation, and texture. KT 20, which was selected as rhizobia isolate with the best potential as PGPR, has ammonium concentration of 23.12 ppm, synthesizes IAA with a concentration of 10.36 ppm, and phosphates solubilization activity, although its ability to synthesize proteases is low. The results of molecular identification of 16S rRNA showed that KT 20 belongs to the Rhizobium genus with a similarity of 99.48 % and bootstrap value of 96 %. HIGHLIGHTS Isolate KT 20 (identified rhizobia) has excellent ability in fixing nitrogen, which plants needs the most and legumes requires in large number Beside the ability of Isolate KT 20 to fix nitrogen, it also able to synthesize IAA, solubilize phosphate, and synthesize protease The ability of isolate KT 20 as PGPR was equal or better than other rhizobacteria that has been analyzed in other in vitro studies GRAPHICAL ABSTRACT

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Section: S Rrna Molecular Identification Of Rhizobia Isolatementioning

confidence: 99%

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Wulandari,

Baskoro,

Mahmuudah

et al. 2024

Trends Sci

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“…with the ability to develop nodules on the roots, and occasionally the stems of host plants, which are mostly legumes (Ahemad and Kibret, 2014;Raissa et al, 2020). The alpha-proteobacteria harbour most bacterial genera of nodule-forming microsymbionts; for example, the major genera are Rhizobium, Bradyrhizobium, Azorhizobium, Mesorhizobium, Ensifer (Sinorhizobium), Neorhizobium, Pararhizobium, and Allorhizobium, collectively referred to as "rhizobia" (Jaiswal et al, 2021), which can form symbiotic associations with a variety of legumes.…”

Section: Biological Nitrogen Fixationmentioning

confidence: 99%

Prospects of rhizobial inoculant technology on Bambara groundnut crop production and growth

et al. 2022

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After peanuts and cowpeas (Vigna unguiculata), the Bambara groundnut (Vigna subterranea (L.) Verdc) is the third most significant food legume in Africa. It is characteristically grown in marginal soils, is drought tolerant, and also has the potential for nitrogen fixation. Despite that, year-on-year Bambara groundnut yields are on a gradual decline due to a combination of abiotic and biotic stresses such as uneven annual rainfall and climate-induced changes in soil microbial community compositions, negatively impacting food security. Thus, the application of rhizobial inoculants at planting significantly improves yields in many leguminous crops. Moreover, symbiotic inoculants are well established in developed countries for improving nitrogen fixation and productivity in grain legumes. Sub-Saharan African countries, however, still under-utilise the above practice. In crop production, nitrogen (N) is the most frequently deficient nutrient since it stimulates root and shoots growth. Whereas nitrogen fertilisers can be used to supplement soil N levels, they are, however, also costly, at times inadequate, may not be timely in supply and may have deleterious environmental consequences. Hence, rhizobial inoculants are seen as a cheaper, easier, and safer method for improving N-fixation and crop productivity in grain legumes, as a result, smallholder farming systems are food secure. Thus, identifying the most efficient rhizobial strains for biofertiliser production for Bambara groundnut is of utmost importance to the farming communities.

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Molecular and Symbiotic Efficiency Characterization of Rhizobia Nodulating Bambara Groundnut (Vigna subterranea L.) from Agricultural Soils of Daloa Localities in Côte D’ivoire

Cited by 2 publications

References 25 publications

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Bioprospecting of Rhizobia as Plant Growth Promoting Rhizobacteria Potential from Root Nodules of Groundnut (Arachis hypogaea L.)

Prospects of rhizobial inoculant technology on Bambara groundnut crop production and growth

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