Physiology of IgD. II. Lack of humoral immune responsiveness in lymph nodes of mice treated with anti‐IgD from birth

Baine, Yaela; Chen, YW; Eb, Jacobson; Pernis, B; Gw, Siskind; Thorbecke, G. J.

doi:10.1002/eji.1830121015

Cited by 4 publications

(4 citation statements)

References 22 publications

(11 reference statements)

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“…That the number of circulating ,L u, + B cells was almost normal, even in mice immunized at 2 days of age, suggests that the circulating anti-IgD did not significantly downregulate B-cell maturation; this contrasts with previous observations on mice injected with homologous allo-anti-IgD from birth (5,20,21).…”

Section: Discussioncontrasting

confidence: 64%

“…For example, it has been shown that treatment of mice from birth with allogeneic anti-IgD suppresses cell-surface IgD expression and down-regulates the production of antibodies of all isotypes to antigens administered s.c. or i.p. (4,5,8). However, the greatly reduced numbers of remaining 4+, 5-B cells in such mice were shown to be actually hyperresponsive to antigens injected i.v.…”

mentioning

confidence: 99%

“…The significance of surface IgD in regulation has been demonstrated in many investigations (4)(5)(6)(7)(8)(9)(10). For example, it has been shown that treatment of mice from birth with allogeneic anti-IgD suppresses cell-surface IgD expression and down-regulates the production of antibodies of all isotypes to antigens administered s.c. or i.p.…”

mentioning

confidence: 99%

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Induction and properties of syngeneic murine anti-immunoglobulin D.

Haba

Thorbecke²,

Nisonoff³

1988

Proc. Natl. Acad. Sci. U.S.A.

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High titers of autoantibodies directed to isotypic determinants of IgD were produced by inoculation of syngeneic monoclonal IgD, conjugated covalently to keyhole limpet hemocyanin, into adult or neonatal inbred mice. Antiidiotypic antibodies were induced at the same time. The average affinity of the mouse antibodies (Ka 9 107 M-1) is similar to that of rabbit anti-IgD and of syngeneic anti-IgE induced by the same procedure. Results indicate that B cells of the mice are not tolerant to serum IgD and that tolerance is maintained at the level of T cells. Direct interaction of the syngeneic anti-IgD with cell-surface IgD was minimal, and there was no convincing evidence that cell-surface IgD was down-regulated in the anti-IgD-producing mice. Further studies, preferably employing monoclonal anti-IgD, are required to determine whether epitopes on cell-surface IgD can be recognized by syngeneic anti-IgD. The ability to generate in vivo high titers of anti-IgD should facilitate the production of such monoclonal antibodies.

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Section: Discussioncontrasting

confidence: 64%

mentioning

confidence: 99%

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Induction and properties of syngeneic murine anti-immunoglobulin D.

Haba

Thorbecke²,

Nisonoff³

1988

Proc. Natl. Acad. Sci. U.S.A.

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“…These cells responded normally to a thymus-independent antigen given i.v. but were apparently unable to migrate to antigen-stimulated LN either in the same animal or after transfer to immunosuppressed recipients [22]. It seems probable that they are identical to the sessile cells demonstrated in the rat and in the present experiments.…”

Section: Nsmentioning

confidence: 67%

Recirculating and sessile B cell populations in normal and CBA/N mice

Chaudhuri¹,

Wallis²,

Connell³

1983

Eur J Immunol

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Chromosomally distinguishable syngeneic mice were parabiosed and the resultant chimerism was followed for 6 weeks in the lymphoid organs, by culturing their cells with polyclonal mitogens, lipopolysaccharide (LPS) for B cells and phytohemagglutinin (PHA) for T cells. As expected of a recirculating population, the T cells equilibrated completely. The B cells in lymph nodes (LN) and Peyer's patches (PP) also equilibrated completely, suggesting that they too are recirculating. B cells in the spleen and blood, however, did not equilibrate over this period. After separation of parabiosed mice, the percentage of partner cells in both the recirculating T and B lymphocyte populations declined steadily, but it continued to rise in the LPS-responsive populations in spleen and peripheral blood suggesting that they were derived from precursor populations which were themselves chimeric. Injection of lymphocytes into CBA/Ca or CBA/N mice showed that LPS-responsive populations in LN and spleen localized differently. These results have been interpreted as demonstrating two major populations of LPS-responsive B lymphocytes in the mouse, one recirculating and the other sessile. The recirculating population appears to be the only LPS-responsive population in LN and PP. In the spleen, however, the recirculating cells constitute about a quarter of the LPS-responsive cells, while the rest are sessile cells. The relationship between these two populations has yet to be clarified. CBA/N mice are deficient in both populations but the sessile one appears to be more severely depleted.

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Physiology of IgD III. Effect of treatment with anti‐IgD from birth on the magnitude and isotype distribution of the immune response in the spleen

et al. 1984

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Continued treatment with monoclonal anti-IgD (Ig-5a) from birth in BALB/c mice causes a markedly increased responsiveness to i.v. injected dinitrophenylated ovalbumin (DNP-OVA) with Bordetella pertussis at the age of 8 weeks. The 19S plaque-forming cell (PFC)/spleen response is particularly enhanced, 6-8-fold, but all the other isotypes also show increases of 2-6-fold, including IgA and IgE. Both primary and secondary PFC responses and serum antibody titers are enhanced. After transfer of spleen cells from anti-Ig-treated mice to irradiated recipients the IgM/IgG ratio becomes similar to that of controls. In contrast, the response of anti-IgD-treated mice to i.p. immunization with either 0.2 or 100 micrograms DNP-OVA plus alum is reduced by approximately 80% for each Ig isotype except IgM and remains low upon transfer of spleen cells to recipients. It is concluded that the paucity of B cells in peripheral lymph nodes of the anti-IgD-treated mice causes the low responsiveness to i.p. immunization, but that the IgD- B cells in the spleen are quite able to respond and are, in fact, more responsive than IgD+ B cells. This increased responsiveness, together with the higher IgM/IgG ratios for all Ig isotypes and an otherwise similar order of isotype distribution (gamma 1 greater than gamma 2b greater than gamma 2a = epsilon greater than or equal to alpha) as in controls, suggests that a hyperresponsive, but less mature IgD- B cell population is selectively produced in the spleens of mice treated with anti-IgD from birth.

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Physiology of IgD. II. Lack of humoral immune responsiveness in lymph nodes of mice treated with anti‐IgD from birth

Cited by 4 publications

References 22 publications

Induction and properties of syngeneic murine anti-immunoglobulin D.

Induction and properties of syngeneic murine anti-immunoglobulin D.

Recirculating and sessile B cell populations in normal and CBA/N mice

Physiology of IgD III. Effect of treatment with anti‐IgD from birth on the magnitude and isotype distribution of the immune response in the spleen

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