Physiology of IgD III. Effect of treatment with anti‐IgD from birth on the magnitude and isotype distribution of the immune response in the spleen

Xue, Bin; Hirano, Toshio; Pernis, B; Óváry, Zoltán; Thorbecke, G. J.

doi:10.1002/eji.1830140115

Cited by 11 publications

(8 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Previous studies of the splenic B cell population of mice treated chronically from birth with anti4 antibody have demonstrated that these cells share some phenotypic and functional characteristics with splenic B cells from neonatal mice [l, 3, [12,37,391, low-angle light scatter analyses of the donor cells which home to the recipient spleens showed that these cells not only failed to express increased sIa densities, but actually exhibited reduced numbers of sIa molecules (M.…”

Section: Discussionmentioning

confidence: 99%

Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

1987

View full text Add to dashboard Cite

To determine whether the expression of surface IgD (sIgD) influences the extent of the expressed B cell repertoire, the clonal diversity of the B cell population in mice treated chronically with anti-IgD (delta) antibodies has been compared with the B cell repertoire observed in control animals, using the splenic focus limiting dilution B cell assay. The results show that the phosphorylcholine (PC)-specific B cell precursor frequency in anti-delta antibody-treated mice is increased when compared with that of control mice. Isotype and idiotype (T15) analyses of PC clonal products from anti-delta antibody-treated and control mice revealed no distributional differences. Analyses of the 2,4-dinitrophenyl (DNP)- and fluoresein isothiocyanate-specific B cell repertoires confirmed that the equal or increased precursor frequencies observed in anti-delta antibody-treated mice are not specific for the PC antigen. The increased precursor frequency of B cells from anti-delta antibody-treated mice was not the result of increased homing of B cells from anti-delta antibody-treated mice to recipient spleens, since B cells from control mice homed twice as well to recipient spleens as did B cells from anti-delta antibody-treated mice. Other studies demonstrated that (a) on average, antibody-secreting clones were generated more slowly when B cells from anti-delta antibody-treated mice were used as a source of precursors than B cells of control mice and (b) both sIg- spleen cells and sIg+ spleen cells from anti-delta antibody-treated mice generated a higher frequency of specific antibody-secreting clones than did the corresponding populations from control mice. These observations suggest that a population of sIgM+sIgD- B cells exists that resembles sIgD+ B cells rather than neonatal or xid B cells in its ability to generate responses to PC and suggests that the sIgM+sIgD- B cells from anti-delta antibody-treated mice are more responsive than are sIgM+IgD+ B cells, regardless of antigenic specificity, to the stimuli provided in the splenic focus system. Finally, this study suggests that the expression of sIgD does not influence the extent of the expressed B cell repertoire.

show abstract

Section: Discussionmentioning

confidence: 99%

Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

1987

View full text Add to dashboard Cite

show abstract

“…For example, it has been shown that treatment of mice from birth with allogeneic anti-IgD suppresses cell-surface IgD expression and down-regulates the production of antibodies of all isotypes to antigens administered s.c. or i.p. (4,5,8). However, the greatly reduced numbers of remaining 4+, 5-B cells in such mice were shown to be actually hyperresponsive to antigens injected i.v.…”

mentioning

confidence: 99%

“…The significance of surface IgD in regulation has been demonstrated in many investigations (4)(5)(6)(7)(8)(9)(10). For example, it has been shown that treatment of mice from birth with allogeneic anti-IgD suppresses cell-surface IgD expression and down-regulates the production of antibodies of all isotypes to antigens administered s.c. or i.p.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Induction and properties of syngeneic murine anti-immunoglobulin D.

Haba

Thorbecke²,

Nisonoff³

1988

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

High titers of autoantibodies directed to isotypic determinants of IgD were produced by inoculation of syngeneic monoclonal IgD, conjugated covalently to keyhole limpet hemocyanin, into adult or neonatal inbred mice. Antiidiotypic antibodies were induced at the same time. The average affinity of the mouse antibodies (Ka 9 107 M-1) is similar to that of rabbit anti-IgD and of syngeneic anti-IgE induced by the same procedure. Results indicate that B cells of the mice are not tolerant to serum IgD and that tolerance is maintained at the level of T cells. Direct interaction of the syngeneic anti-IgD with cell-surface IgD was minimal, and there was no convincing evidence that cell-surface IgD was down-regulated in the anti-IgD-producing mice. Further studies, preferably employing monoclonal anti-IgD, are required to determine whether epitopes on cell-surface IgD can be recognized by syngeneic anti-IgD. The ability to generate in vivo high titers of anti-IgD should facilitate the production of such monoclonal antibodies.

show abstract

“…Goat anti-mouse IgM (specific against the p chain) and rabbit anti-mouse immunoglobulin (Ig, the 50% ammonium-sulfate-injoluble fraction) were a generous gift of Dr. Thorbecke (Depart ment of Pathology, NYU Medical School [II,12]). Rabbit anti mouse Ig and IgE were previously described [9].…”

Section: Antisera Used For Pfc and Preparation O F Immunoadsorbent Comentioning

confidence: 99%

Studies on Immunity in Hybridoma-Bearing Mice A. Immune Response to Antigens

Yin¹,

Furusawa²,

Hirano³

et al. 1987

Int Arch Allergy Immunol

Self Cite

View full text Add to dashboard Cite

When BALB/c mice, which had rejected the anti-dinitrophenyl (DNP) IgE-producing hybridoma B 53, were immunized with DNP proteins, they produced much less anti-DNP antibodies than control (normal) mice. The anti-DNP plaque-forming cell (PFC) number was much less when spleen cells from mice immunized with DNP proteins were treated with sera of mice which had rejected the hybridoma B 53 than the PFC number from the same spleen cells not treated by the sera. The sera of mice which had rejected the hybridoma B 53 contained an inhibitor which was adsorbed and eluted from an anti-mouse immunoglobulin column and also a mouse anti-DNP IgG2a column. The inhibition of PFC was hapten-reversible. In Western blotting the eluates from the anti-DNP IgG2a column reacted as well with the blotted anti-DNP IgE B 53 as an anti-idiotypic antibody to anti-DNP IgE B 53. These criteria establish that the inhibitor in the sera of the mice which had rejected the B 53 tumor was an anti-idiotypic antibody of the type which mimics the epitope (DNP) of the immunizing antigen.

show abstract

Physiology of IgD III. Effect of treatment with anti‐IgD from birth on the magnitude and isotype distribution of the immune response in the spleen

Cited by 11 publications

References 29 publications

Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

Comparison of the clonal diversity of the B cell repertoires in adult mice that differ in the expression of cell surface IgD

Induction and properties of syngeneic murine anti-immunoglobulin D.

Studies on Immunity in Hybridoma-Bearing Mice A. Immune Response to Antigens

Contact Info

Product

Resources

About