Physiological Study and both in vitro and in vivo Antifungal Activities against Stemphylium botryosum causing Stemphylium Blight Disease in Lentil (Lens culinaris)

Rahman, Tanjina; Ahmed, Ashfaq; Islam, M. R.; Hosen, Md. Iqbal

doi:10.3923/ppj.2010.179.187

Cited by 18 publications

(14 citation statements)

References 2 publications

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“…This finding is also well supported by the observation that stated S. botryosum varied in their colony color, texture, margin, shape and also size of conidia on PDA medium while worked on 4 isolates and measured the conidia as 13.33-16.04× 6.46-9.17 µm [10]. The result is also in agreement with the report that the optimum temperature for radial mycelia growth of S. botryosum was 25 0 C [11]. Temperature plays an important role in influencing the growth of fungi [12].…”

Section: Interaction Effect Between Temperature and Isolatessupporting

confidence: 90%

Effect of incubation temperatures on mycelial growth, conidial features and density of Stemphylium botryosum Walr isolates

Sasidharan¹,

Saraswati²

2018

Kathmandu University J of Sci, Engineering & Technol

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The cultural and morphological variability of Stemphylium botryosum Walr was studied with five isolates viz. isolate 1 (Stb-I1), isolate 2 (Stb-I2), isolate 3 (Stb- I3), isolate 4 (Stb-I4) and isolate 5 (Stb-I5) collected from Parsa, Rautahat, Dang, Banke and Chitwan districts of Nepal respectively during 2011-2012 crop season with Completely Randomized Design (CRD) maintaining 3 replications at Plant Pathology Laboratory of Grain Legumes Research Program (GLRP), Rampur, Chitwan. Single spore isolation technique was followed to isolate the pathogen. The pathogen was grow on Potato Dextrose Agar (PDA) and incubated with different temperatures in BOD incubator. The calibrated ocular micrometer was used to measure the length and breadth of the conidia while conidial count with sporulation intensity was attempted with hemocytometer. The mycelium color of the different isolates was varied from white, grey, brown and brownish black in peripheral part while mostly black pigmentation was noticed in lateral part of the culture plate. The shape and texture was regular and velvety for most isolates and in some isolate, roughly irregular shape with cottony texture was found. The higher colony diameter of 7.66 cm and conidial dimension of 29.42×18.12 μm (L/B ratio-1.62) with profuse sporulation intensity (46.67 conidia /0.01 ml) was observed at 25 °C after 15 days of incubation on PDA. However, 2.84 cm diameter, 19.58×9.81 μm (L/B ratio-2.00) with poor conidial density (7.60 conidia /0.01 ml) was noticed at 10 °C. The observation at 10 and 35 °C was at par in case of mycelial growth, conidial features and sporulation intensity. Mostly conidia of all isolates were brown in color at lower incubation temperature but they became dark grey brown at 25 and 30 °C and grey color was noticed when incubated at 35 °C on PDA after 15 days of incubation.Kathmandu University Journal of Science, Engineering and TechnologyVol. 12, No. I, June, 2016, page: 80-89

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Section: Interaction Effect Between Temperature and Isolatessupporting

confidence: 90%

Effect of incubation temperatures on mycelial growth, conidial features and density of Stemphylium botryosum Walr isolates

Sasidharan¹,

Saraswati²

2018

Kathmandu University J of Sci, Engineering & Technol

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“…On the other hand Trichoderma isolates were effective more than Bacillus isolates in inhibition of redial growth of all the tested fungi and the greatest reduction occurring in D. halodes (63.07%) followed by F. graminearum (54.77%), while R. solani less affected ones (50.07%). The growth inhibition of wheat root rot fungi by dual culture in this study could be due to its fast growing nature, secretions of armful extra-cellular compounds like antibiotics, cell wall degrading enzymes such as β-1, 3 gluconase, endochitinase and chitinase enzymes which degrade the cell wall leading to lyses of mycelium of the pathogen and mycoparasitism in dual culture as found with other fungi (Lugtenberg et al, 2003;Rahman et al, 2010;Riungu et al, 2008).…”

Section: Efficacy Of Antagonistic Bioagents Against Pathogenic Fungi mentioning

confidence: 75%

Effect of Bio-Control Agents on Yield, Yield Components and Root Rot Control in two Wheat Cultivars at New Valley Region

Moubark

Abdel-Monaim

2011

Not Sci Biol

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This study evaluated the effectiveness of applying the bioagents Bacillus subtilis (isolate BSM1), B. megaterium (isolate BMM5), Trichoderma viride (isolate TVM2) and T. harzianum (isolate THM4) for the control of rot root disease caused by Fusarium graminearum, Drechslera halodes and Rhizoctonia solani on two wheat cultivars ('Sakha 93' and 'Bani Suif 5') under greenhouse conditions. Moreover, their effect wheat growth and yield were also studied under field conditions. In vitro, all tested bioagents were significantly in the reduced redial growth of the pathogenic fungi. Trichoderma viride was active more than the other tested bioagents followed by T. harzianum, while B. subtilis was the least ones. Under greenhouse conditions, all tested bioagents were able to reduce significantly damping-off and root rot caused by the tested pathogens compared with control and increased fresh and dry weight of the survival plants when applied as soil or grain treatments however, there was variation among bioagent isolates effect on reduction of disease severity both application methods. Trichoderma viride and B. megaterium were recorded the highest effective in this respect compared with other tested bioagents. Under field conditions, analysis of variance and mean performance were estimated for four characters: grain yield 4.8 m S), methods (M), (S) (M), treatments (T), (S) (T), (M) (T) and (S) (M) (T)for 'Sakha 93' cultivar and 'Bani Suif 5' cultivars except application methods and (S) (M) for number of kernels spike -1 and 1000-kernel weight, respectively. While the treatments T. harzianum and B. megaterium were best treatments to increase grain yield, the treatments B. subtilis and T. harzianum were best treatments to increase number of spikes and the treatments B. subtilis and B. megaterium were best treatments to increase number of kernels for soil and grain application methods, respectively.

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“…Lentil is a major grain and widely distributed legume crop grown under a broad range of climates in many developing countries (Turk et al, 2004;Abd-Allah and Hashem, 2006). This crop has been grown mainly as an inexpensive source of high quality protein in human diets (Salehpour et al, 2009;Rahman et al, 2010). In Nepal also, lentil is the most important and highly commercialized pulse among the grain legumes in terms of area (206522 ha), production (226931 mt) and productivity (1099 kg/ha) which shares almost 62% of total area and 65% of total production of pulses and rates the highest consumer preference (MOAD, 2013).…”

Section: Introductionmentioning

confidence: 99%

Influence of sowing dates on stemphylium blight disease severity and yield performance of lentil at Rampur, Chitwan, Nepal

Subedi

Shrestha

Khatri-Chhetri

et al. 2018

J. Inst. Agric. Anim. Sci.

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A study on lentil (Lens culinaris Medik) was carried out at Rampur, Chitwan with different sowing dates under field conditions to assess the severity of Stemphylium blight disease severity and yield performance during two consecutive years 2012-2014. During 2012/013, lower (3.00) and higher disease severity (6.50) were recorded on lentil plants sown on November 6 and December 16, respectively. Early sown lentil resulted in higher yield (2025 kg/ha) followed by late shown one (1213 kg/ha). Similarly, trends of disease development were similar in succeeding year (2013/14). Lentil seed shown on November 1 had lower disease severity (3.50) and produced higher yield (962.50 kg/ha) compared to that were shown on later dates. Disease severity increased with the advancement of sowing date from November 1 to December 21 with decreased yields. Hence, over years, early shown lentil (November 1, 6 and 11) escaped the higher severity of Stemphylium blight with the increase in yield.Journal of the Institute of Agriculture and Animal Science.Vol. 33-34, 2015, Page: 129-136

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Physiological Study and both in vitro and in vivo Antifungal Activities against Stemphylium botryosum causing Stemphylium Blight Disease in Lentil (Lens culinaris)

Cited by 18 publications

References 2 publications

Effect of incubation temperatures on mycelial growth, conidial features and density of Stemphylium botryosum Walr isolates

Effect of incubation temperatures on mycelial growth, conidial features and density of Stemphylium botryosum Walr isolates

Effect of Bio-Control Agents on Yield, Yield Components and Root Rot Control in two Wheat Cultivars at New Valley Region

Influence of sowing dates on stemphylium blight disease severity and yield performance of lentil at Rampur, Chitwan, Nepal

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