Physiological Responses of Apple and Cherry In Vitro Culture under Different Levels of Drought Stress

Kovalíková, Zuzana; Jiroutová, Petra; Toman, Jakub; Dobrovolna, Dominika; Drbohlavová, Lenka

doi:10.3390/agronomy10111689

Cited by 13 publications

(3 citation statements)

References 34 publications

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“…Indeed, Guo et al (2010) reported the inhibitory effect of drought on chlorophyll concentration and intensity of photosynthesis in three hybrid poplar (Populus deltoides × Populus nigra) clones, whereas Jaleel et al (2008) found reduced chlorophyll content in Catharanthus roseus L. plants subjected to water stress. Kovalikova et al (2020) also found a decrease in the content of chlorophyll a and b in leaves of cherry cultured on a growing medium with 50 g L -1 PEG in vitro, as well as a difference in the response of examined cultivars by these parameters. Thus, the reduction of chlorophyll a content and chlorophyll b content caused by water stress in our study, particularly in leaves, is in accordance with the general opinion.…”

Section: Iforest -Biogeosciences and Forestrymentioning

confidence: 71%

Variability of tolerance of Wild cherry clones to PEG-induced osmotic stress in vitro

Vuksanović¹,

Kovačević²,

Stojnić³

et al. 2022

iForest

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The effects of drought simulated via osmotic stress induced by polyethylene glycol (PEG) in the growing medium were examined on two Wild cherry (Prunus avium L.) clones (6A and 8A) based on thirteen morphometric, physiological, and biochemical traits. The shoot tips were exposed to two PEG concentrations (20 and 50 g L -1 ) in growing medium designed for micropropagation with axillary buds. The results showed that all morphological and physiological traits were significantly reduced, indicating a strong detrimental effect of increased PEG concentrations. The significant decline of radical scavenging activity against ABTS •+ and total content of flavonoids (TFC) and phenols (TPC) were recorded in both clones as a response to high PEG concentrations, whereas opposite trends were noticed for ferric reducing antioxidant power (FRAP). Clone 8A achieved better performance, having more intensive growth, higher multiplication percentage, higher values of carotenoids, more intense decrement of ABTS and increment of FRAP values compared to the clone 6A. The results of the principal component analysis indicate that parameter TFC/ TPC in both stem and leaves, as well as TFC in the stems, achieved the strongest relation with morphometric parameters. Our results confirm the feasibility of in vitro evaluation of drought tolerance of Wild cherry, supporting further research on the variability of examined traits in this noble broadleaved tree species.

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Section: Iforest -Biogeosciences and Forestrymentioning

confidence: 71%

Variability of tolerance of Wild cherry clones to PEG-induced osmotic stress in vitro

Vuksanović¹,

Kovačević²,

Stojnić³

et al. 2022

iForest

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“…The non-toxicity of PEG 6000 in relation to living objects and its lack of participation make it promising for use in laboratory evaluation of the tree crops' sustainability. PEG 6000 reduces the water potential of the nutrient medium but does not penetrate the cell itself (Kovalikova et al, 2020, Mohanlal et al, 2021. The PEG effect includes the structural and functional restructuring of the photosynthetic apparatus and inhibition of photosynthesis.…”

Section: Screening Smoke Tree (Cotinus Coggygria Scop) On Osmotic Str...mentioning

confidence: 99%

Screening Smoke Tree (Cotinus coggygria Scop.) on Osmotic Stress using Polyethylene Glycol 6000 in vitro

Zholobova,

Mogilevskaya,

Melnik

2023

IJARe

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Background: Droughts during the growing season are becoming increasingly common due to climate change and global warming. The study’s goal study was to assess the adaptation mechanisms of C. coggygria Scop. under simulated water deficit conditions using indicators characterized changes in the leaf stomatal apparatus and photosynthesizing system. Methods: The influence of osmotic polyethylene glycol 6000 on the research object C. coggygria regenerants was studied in vitro. Such indicators as morphological changes, pigment content, stomatal density and size and stomatal slit size were quantified. Result: Due to the influence of osmotic stress, there was a pigment content increase in the leaf plate on osmotic media. Stomatal density raised by 27.0-29.8% in explant cells cultivated on PEG 4.0-6.0%. Under the osmotic influence, there was a drop in the stomatal cells’ area and shape and the ratio of stomatal length to width in the regenerant leaves decreased from 1.5 to 1.0-1.1. These results indicate the adaptation of C. coggygria regenerants to stress caused by an artificial drought in vitro. The changes’ identification in the stomatal apparatus and the C. coggygria pigment ratio will accelerate the selective screening of drought-resistant plants for use in protective forestry.Background: Droughts during the growing season are becoming increasingly common due to climate change and global warming. The study’s goal study was to assess the adaptation mechanisms of C. coggygria Scop. under simulated water deficit conditions using indicators characterized changes in the leaf stomatal apparatus and photosynthesizing system.

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“…Nowadays, implementing in vitro water stress experiments based on polyethylene glycol (PEG) treatments is frequently used to identify drought tolerance genotypes in higher plants (Kovalikova et al, 2020). PEG, an inert non-penetrating and nonionic polymer with a high molecular weight (4000-8000 Da) and soluble in water, can decrease the water potential of the substrate by inducing osmotic stress without being phytotoxic (Emmerich and Hardegree, 1990;Ahmad et al, 2020) as it happens under field conditions.…”

Section: Introductionmentioning

confidence: 99%

Tetraploidy Confers Superior in vitro Water-Stress Tolerance to the Fig Tree (Ficus carica) by Reinforcing Hormonal, Physiological, and Biochemical Defensive Systems

Abdolinejad

Shekafandeh

2022

Front. Plant Sci.

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The fig tree is a well-adapted and promising fruit tree for sustainable production in arid and semi-arid areas worldwide. Recently, Iran’s dryland fig orchards have been severely damaged due to prolonged severe and consecutive drought periods. As emphasized in many studies, ploidy manipulated plants have a significantly enhanced drought tolerance. In the current study, we compared the induced autotetraploid explants of two fig cultivars (‘Sabz’ and ‘Torsh’) with their diploid control plants for their water stress tolerance under in vitro conditions using different polyethylene glycol (PEG) concentrations (0, 5, 10, 15, 20, and 25%). After 14 days of implementing water stress treatments, the results revealed that both tetraploid genotypes survived at 20% PEG treatments. Only ‘Sabz’ tetraploid explants survived at 25% PEG treatment, while both diploid control genotypes could tolerate water stress intensity only until 15% PEG treatment. The results also demonstrated that the tetraploid explants significantly had a higher growth rate, more leaf numbers, and greater fresh and dry weights than their diploid control plants. Under 15% PEG treatment, both tetraploid genotypes could maintain their relative water content (RWC) at a low-risk level (80–85%), while the RWC of both diploid genotypes drastically declined to 55–62%. The ion leakage percentage also was significantly lower in tetraploid explants at 15% PEG treatment. According to the results, these superiorities could be attributed to higher levels of stress response hormones including abscisic acid, salicylic acid, and jasmonic acid at different PEG treatments, the robust osmotic adjustment by significantly increased total soluble sugar (TSS), proline, and glycine betaine contents, and augmented enzymatic defense system including significantly increased superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), and glutathione peroxidase (GPX) activities in tetraploid genotypes, compared to their diploid control genotypes. Consequently, the current study results demonstrated that the ‘Sabz’ tetraploid genotype had a significantly higher water stress tolerance than other tested genotypes.

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Physiological Responses of Apple and Cherry In Vitro Culture under Different Levels of Drought Stress

Cited by 13 publications

References 34 publications

Variability of tolerance of Wild cherry clones to PEG-induced osmotic stress in vitro

Variability of tolerance of Wild cherry clones to PEG-induced osmotic stress in vitro

Screening Smoke Tree (Cotinus coggygria Scop.) on Osmotic Stress using Polyethylene Glycol 6000 in vitro

Tetraploidy Confers Superior in vitro Water-Stress Tolerance to the Fig Tree (Ficus carica) by Reinforcing Hormonal, Physiological, and Biochemical Defensive Systems

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