Variability of tolerance of Wild cherry clones to PEG-induced osmotic stress in vitro

Abstract

The effects of drought simulated via osmotic stress induced by polyethylene glycol (PEG) in the growing medium were examined on two Wild cherry (Prunus avium L.) clones (6A and 8A) based on thirteen morphometric, physiological, and biochemical traits. The shoot tips were exposed to two PEG concentrations (20 and 50 g L -1 ) in growing medium designed for micropropagation with axillary buds. The results showed that all morphological and physiological traits were significantly reduced, indicating a strong detrimental effect of increased PEG concentrations. The significant decline of radical scavenging activity against ABTS •+ and total content of flavonoids (TFC) and phenols (TPC) were recorded in both clones as a response to high PEG concentrations, whereas opposite trends were noticed for ferric reducing antioxidant power (FRAP). Clone 8A achieved better performance, having more intensive growth, higher multiplication percentage, higher values of carotenoids, more intense decrement of ABTS and increment of FRAP values compared to the clone 6A. The results of the principal component analysis indicate that parameter TFC/ TPC in both stem and leaves, as well as TFC in the stems, achieved the strongest relation with morphometric parameters. Our results confirm the feasibility of in vitro evaluation of drought tolerance of Wild cherry, supporting further research on the variability of examined traits in this noble broadleaved tree species.

Metal- and Organ-Specific Response to Heavy Metal-Induced Stress Mediated by Antioxidant Enzymes’ Activities, Polyamines, and Plant Hormones Levels in Populus deltoides

“…A concentration of 200 μM caused a 47% increase in phenolic compounds in the roots of Populus deltoides and a 38–168% increase in the bark of Populus ×euramericana , Populus nigra , and Populus popularis , while in the leaves of Populus nigra, phenolic content was 67% higher compared to control plants [ 59 ]. Phenolic compounds increase plant tolerance to various abiotic stress factors, such as temperature fluctuations, the presence of heavy metals [ 68 ], and water deficit [ 69 , 70 ], in addition to their importance in allelopathic relationships and herbivore defense [ 71 ]. Furthermore, other publications revealed that the total polyphenolic content of many plants reduced during abiotic stresses [ 72 , 73 , 74 , 75 ].…”

Ecophysiological response of Populus alba L. to multiple stress factors during the revitalisation of coal fly ash lagoons at different stages of weathering

“…By regulating the transfer between soil and roots and between roots and leaves, concentrations of these elements are kept within a normal range in their tissue ( Pandey et al., 2012 ; Mukhopadhyay et al., 2017 ; Kostić et al., 2022b ). Tolerant plant species also respond to abiotic stress by increasing their antioxidant capacity, which helps them to maintain a normal cellular balance between production and binding, degradation, and neutralisation of ROS by enzymatic or non-enzymatic antioxidants (carotenoids and anthocyanins) and secondary metabolites (phenols) ( Cervilla et al., 2012 ; Gajić et al., 2020 ; Kebert et al., 2022 ; Vuksanović et al., 2022 ). Intolerant species exibit decreased antioxidant capacity and fewer mechanisms for detoxification in relation to ROS production, resulting in chain reactions in which free radicals damage important biomolecules, such as chloroplast pigments, lipids, and nucleic acids, oxidatively.…”

In vitro selection of drought-tolerant white poplar clones based on antioxidant activities and osmoprotectant content

“…In vitro cultures have been used to assess drought tolerance in numerous plant species: potato (Solanum tuberosum L.) (Hanaśz et al, 2022), rice (Biswas et al, 2002), guava (Psidium guajava L.) (Youssef et al, 2016), blueberry (Vaccinium corymbosum L.) (Molnar et al, 2022), "Mexican lime" (Citrus aurantifolia (Christ.) Swingle) (Jafari and Shahsavar, 2022), Mexican marigold (Tagetes minuta L.) (Babaei et al, 2021), Stevia rebaudiana (Bertoni) (Hajihashemi and Sofo, 2018), Agave salmiana (Puente-Garza et al, 2017), Wild cherry (Prunus avium L.) (Vuksanovićet al, 2022), olives (Olea europaea L.) (Silvestri et al, 2017), kiwifruit (Actinidia chinensis Planch. ), (Wu et al, 2019), fig tree (Ficus carica L.) (Abdolinejad and Shekafandeh, 2022) and euramerican poplar (Popovićet al, 2017).…”