Physiological responses and expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress

Ghouili, Emna; Sassı, Khaled; Jebara, Moez; Hidri, Yassine; Ouertani, Rim Nefissi; Muhovski, Yordan; Jebara, Salwa Harzalli; Ayed, Mohamed El; Abdelkarim, Souhir; Chaieb, Oumaima; Jallouli, Sélim; Kalleli, Fatma; Mahmoud, Mahmoud A.; Souissi, Fatma; Abid, Ghassen

doi:10.1007/s12298-021-00935-1

Cited by 10 publications

(4 citation statements)

References 72 publications

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“…Malic Acid, one of the substances present, has potential anti-inflammatory activity, reduction of stress-related symptoms, inhibition of superoxide anion and is related to the reduction of neuroinflammation revealed in preclinical studies 47,48 . α-L-Mannofuranose, in turn, seems to be associated with reduced osmotic stress 49 . Myo-Inositol was associated with antidepressant activity in experimental models of depression and a possible role in stress resistance pathways evidenced in studies with plants 50,51 .…”

Section: Phytochemical Evaluation and Gc-ms Resultsmentioning

confidence: 95%

Effectiveness of Tea Made from <i>Cissampelos sympodialis</i> Leaves in the Treatment of Anxiety and Depression: A Controlled Clinical Trial

Barros Mangueira,

Medeiros Negreiros,

Soares Aragão

et al. 2024

JNR

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The species Cissampelos sympodialis Eichl. (CsE) is popularly used for its anti-inflammatory, bronchodilator and anti-allergiceffects. In preclinical studies, the plant has shown anxiolytic and antidepressant properties, which necessitated a controlledclinical trial to verify its effects in humans. A quasi-randomized study was developed in which the volunteers were divided intothree groups: CsE leaves tea (n = 21), auriculotherapy (n = 20) and no intervention (n = 19). The participants in the three groupsreceived weekly sachets of powdered dried leaves of CsE for tea preparation or auriculotherapy or did not receive intervention,respectively. At the beginning and end of the 5-week follow-up, the volunteers completed the following questionnaires: STAI-S,STAI-T, BDI and BAI. The phytochemical analysis of the sachet containing the powdered dried CsE leaves showed the presenceof alkaloids, steroids, tannins and flavonoids. In addition, the chromatographic analysis of CsE leaves identified the presence of7 chemical substances, for instance Malic Acid, α-L-Mannofuranose and Myo-Inositol. The STAI-S, STAI-T, BDI and BAI responsesshowed that the groups using tea or auriculotherapy showed significant reductions in mean scores in the two-way repeatedmeasures analysis of variance. In the no-intervention group, no significant differences were found on any scale. Applying the post-hoc test, the influence of the group on the initial or final variances of the STAI-S (p = 0.004), STAI-T (p = 0.005), BDI (p < 0.0005)and BAI (p = 0.011) data were tested, highlighting that the tea and auriculotherapy led to the decrease in the questionnairescores post-treatment. The tea of leaves of CsE proved, in this study, to be effective in decreasing the scores of STAI-S, STAI-T,BDI and BAI and could represent a new perspective of complementary treatment for the symptoms of anxiety and depression.

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Section: Phytochemical Evaluation and Gc-ms Resultsmentioning

confidence: 95%

Effectiveness of Tea Made from <i>Cissampelos sympodialis</i> Leaves in the Treatment of Anxiety and Depression: A Controlled Clinical Trial

Barros Mangueira,

Medeiros Negreiros,

Soares Aragão

et al. 2024

JNR

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“…Strikingly, LWC after recovery was higher than at the beginning of the experiment, which could be explained by osmotic adjustment during the 5 days of gradual drying resulting in increased osmotic potential and hence higher final water content. In Vica faba , changes in ion fluxes (Shabala et al 2000) and in expression patterns of sugar metabolism related genes conforming to processes of osmotic adjustment (Ghouili et al 2021) were found after exposure to osmotic stress. The reports on the actual existence and magnitude of osmotic adjustment, however, remain contradictory (Amede et al 1999; Khallafallah et al 2008; Abid et al 2017).…”

Section: Resultsmentioning

confidence: 99%

A new device for continuous non-invasive measurements of leaf water content using NIR-transmission allowing dynamic tracking of water budgets

Kaiser

2022

Preprint

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Leaf water content (LWC) permanently fluctuates under variable transpiration rate and sap flow and influences e.g. stomatal responses and osmotic adjustment of plant cells. Continuous recordings of LWC are therefore central for the investigation of the regulatory networks stabilizing leaf hydration. Available measurement methods, however, either influence local hydration, interfere with the local leaf micro-environment or cannot easily be combined with other techniques. To overcome these limitations a non-invasive sensor was developed which uses light transmission in the NIR range for precise continuous recordings of LWC. For LWC measurements the transmission ratio of two NIR wavelengths was recorded using a leaf-specific calibration. Pulsed measurement beams enabled measurements under ambient light conditions. The contact-free sensor allows miniaturization and can be integrated into many different experimental settings. Example measurements of LWC during disturbances and recoveries of leaf water balance show the high precision and temporal resolution of the LWC sensor and demonstrate possible method combinations. Simultaneous measurements of LWC and transpiration allows to calculate petiole influx informing about the dynamic leaf water balance. With simultaneous measurements of stomatal apertures the relevant stomatal and hydraulic processes are covered, allowing insights into dynamic properties of the involved positive and negative feed-back loops.

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“…This is consistent with our study in which JB17 had a higher trehalose and glucose content. Although there is a lack of research on the role of rhamnose in plant resistance to saline and alkaline stress, it has been found in some studies that rhamnose is beneficial in maintaining osmoregulation and enhancing tolerance to osmotic stress in plants ( Ghouili et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Cellulose and JbKOBITO 1 mediate the resistance of NaHCO3-tolerant chlorella to saline-alkali stress

Qiu,

Zhang,

Zhao

et al. 2023

Front. Microbiol.

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Carbonate stress has profound impacts on both agricultural and industrial production. Although a number of salinity-tolerant genes have been reported and applied in plants, there is a lack of research on the role of cell wall-related genes in resistance to carbonate. Likewise, in industry, current strategies have not been able to more effectively address the conflict between stress-induced microalgal biofuel accumulation and microalgal growth inhibition. It is of great significance to study the adaptation mechanism of carbonate-tolerant organisms and to explore related genes for future genetic modification. In this study, the role of the cell wall in the NaHCO3-tolerant chlorella JB17 was investigated. We found that JB17 possesses a relatively thick cell wall with a thickness of 300–600 nm, which is much higher than that of the control chlorella with a thickness of about 100 nm. Determination of the cell wall polysaccharide fractions showed that the cellulose content in the JB17 cell wall increased by 10.48% after NaHCO3 treatment, and the decrease in cellulose levels by cellulase digestion inhibited its resistance to NaHCO3. Moreover, the saccharide metabolome revealed that glucose, rhamnose, and trehalose levels were higher in JB17, especially rhamnose and trehalose, which were almost 40 times higher than in control chlorella. Gene expression detection identified an up-regulated expressed gene after NaHCO3 treatment, JbKOBITO1, overexpression of which could improve the NaHCO3 tolerance of Chlamydomonas reinhardtii. As it encodes a glycosyltransferase-like protein that is involved in cellulose synthesis, the strong tolerance of JB17 to NaHCO3 may be partly due to the up-regulated expression of JbKOBITO 1 and JbKOBITO 1-mediated cellulose accumulation. The above results revealed a critical role of cellulose in the NaHCO3 resistance of JB17, and the identified NaHCO3-tolerance gene will provide genetic resources for crop breeding in saline-alkali soils and for genetic modification of microalgae for biofuel production.

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Physiological responses and expression of sugar associated genes in faba bean (Vicia faba L.) exposed to osmotic stress

Cited by 10 publications

References 72 publications

Effectiveness of Tea Made from <i>Cissampelos sympodialis</i> Leaves in the Treatment of Anxiety and Depression: A Controlled Clinical Trial

Effectiveness of Tea Made from <i>Cissampelos sympodialis</i> Leaves in the Treatment of Anxiety and Depression: A Controlled Clinical Trial

A new device for continuous non-invasive measurements of leaf water content using NIR-transmission allowing dynamic tracking of water budgets

Cellulose and JbKOBITO 1 mediate the resistance of NaHCO3-tolerant chlorella to saline-alkali stress

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