Physiological regulation for enhancing biosynthesis of biofilm-inhibiting secondary metabolites in Streptomyces cellulosae

Abstract

Although the production of the secondary metabolite is frequently restricted, methods to regulate and optimize their synthesis are extremely beneficial. The current study proposes to enhance the production of antibiofilm metabolite in Streptomyces cellulosae (S. cellulosae). It was isolated from soil by growing on Gause's media and identified by colony morphology and genomic sequencing of 16S rDNA. Antibacterial and antibiofilm activities of the isolates were screened against a series of pathogenic bacteria by agar plug diffusion and 96 well microtiter plate methods, respectively. Physiological regulation of the bacterial bioactivity against biofilm formation was monitored under different cultural conditions. The isolated Streptomyces sequence analysis of the 16S rDNA was 100% identical to the sequence of S. cellulosae strain NBRC 13027. Physical (temperature and pH) and chemical (carbon, nitrogen, and minerals) culture medium factors have shown variable impacts on the growth and bioactive substances of S. cellulosae. Moreover, results of simple linear regression and correlation suggested that most of the physiological regulations with the highest response (r2= 0.85-0.99; p<0.01) and linearly (r= 0.88-0.99; p<0.01) were correlated between microbial biomass and crude extract. Lastly, under different culture growth conditions, biofilm inhibition was tested against Pseudomonas aeruginosa (P. aeruginosa). The physiological regulation results exhibited that 1 μg/mL of the extract was the most efficient concentration against biofilm formation in P. aeruginosa while 3 μg/mL is an effective bactericidal dose against P. aeruginosa. We concluded that S. cellulosae can produce antibacterial and antibiofilm metabolites. Physiological regulation is considered a powerful tool that can be used for increasing the biosynthesis of the active metabolites and biomass.

Efficacy of Crude Extract from Streptomyces cellulosae Against Biofilm-Related Genes of Candida albicans

“…The bacterium was cultivated on Gause's medium after being extracted from soil and identified using colony morphology and 16S rDNA genomic sequencing. Sequence analysis confirmed that the bacteria matched those in the NBRC 13027 strain (6). Clinical samples for isolating C. albicans were collected from Hiwa Hematology/Oncology Hospital in Sulaymaniyah, Iraq.…”

“…Streptomyces cellulosae (S. cellulosae), a species of Actinobacteria known for producing bioactive compounds with antimicrobial properties, has shown potential against various pathogens, including fungi and harmful Gram-positive bacteria (1,2). Notably, Mahmood et al found that S. cellulosae produces compounds that inhibit biofilm formation by Pseudomonas aeruginosa (6).…”

Membranolytic Activity Profile of Nonyl 3,4-Dihydroxybenzoate: A New Anti-Biofilm Compound for the Treatment of Dermatophytosis

“…Most infections caused by microorganisms are associated with the formation of sessile communities and three-dimensional structures covered by a complex polymeric extracellular matrix [ 23 , 24 , 25 ]. This phenotype promotes advantageous protection for microorganisms, which is provided by physical and genetic factors such as the barrier effect of biomass, microenvironment composition, concentration of metabolites and gases, and modification of gene regulation [ 25 , 26 , 27 ].…”