Physiological quality of hybrid maize seeds through respiratory and enzymatic activities

Heloisa, Oliveira dos Santos; Iolanda, Vilela Von Pinho; dila, Vilela de Resende Von Pinho Eacute; Raquel, María; ria, Fabiana da Silva Valk iacute; Maria, Laene Moreira de Carvalho; Roseane, Maria Evangelista Oliveira

doi:10.5897/ajar2015.10457

Cited by 7 publications

(6 citation statements)

References 16 publications

(18 reference statements)

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“…The results obtained were similar to those obtained by Santos et al (2016) who verified significant correlations between the physiological quality of seeds of maize hybrids and the respiratory activity evaluated by the Pettenkofer and titration methods.…”

Section: Resultssupporting

confidence: 88%

Respiratory Activity as a Fast Method for Vigor Differentiation of Habanero Pepper Seeds

Pereira¹,

Freitas²,

Santos³

et al. 2019

JAS

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The objective of this study was to evaluate the efficiency and rapidity of the Pettenkofer and Titulation methods in the determination of respiratory activity and its relation with the vigor of habanero pepper seeds. For this, six lots of habanero pepper seeds were evaluated for respiratory activity, through Pettenkofer and Titulation physicochemical methods, and for physiological quality via tests of germination, first germination count, germination speed index, emergence, initial stand, emergence speed index and electrical conductivity. Biochemical analyzes were also carried out using the electrophoresis of the esterase (EC 3.1.1.1.) and alcohol dehydrogenase (EC 1.1.1.2.), and the quantification of the endo-β-mannanase enzyme (EC 3.2.1.78). The tests of physiological quality and the respiratory activity of the seeds allowed the classification of the habanero pepper seed lots at different vigor levels. There was a correlation between the respiratory activity with the tests used to evaluate the physiological quality of the seeds and with the endo-β-mannanase enzyme. It is concluded that the Pettenkofer and Titulation methods are promising for the evaluation of the deterioration level and the discrimination of lots of habanero pepper seeds with different vigor levels.

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Section: Resultssupporting

confidence: 88%

Respiratory Activity as a Fast Method for Vigor Differentiation of Habanero Pepper Seeds

Pereira¹,

Freitas²,

Santos³

et al. 2019

JAS

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“…Given the above, it is interesting to consider an increase in the respiratory rate of seeds under stress, where those more vigorous lots showed higher efficiency in the use of the energy released by the respiration. According to this, some researchers observed that vigorous seeds have higher respiratory activity in comparison to those less vigorous seeds, in the same situation and period, showing membrane integrity (Dranski et al, 2013;Venske et al,2014;Santos et al, 2016). Bewley and Black (2012) described that the mitochondrion integrity in viable embryos increases since the beginning of imbibition process, where is more efficient in the ATP (adenosine triphosphatase) production.…”

Section: Resultsmentioning

confidence: 96%

“…There are differences in the amount of energy available to seeds and their RDMY (Pereira et al, 2015). Some authors have reported that these variables are strongly influenced by seed size and initial weight (Pádua et al, 2010;Pereira et al, 2013). Pádua et al (2010) and Pereira et al (2013) correlated a larger seed size with better seedling development, indicating the superiority of large seeds in stand formation.…”

Section: Resultsmentioning

confidence: 99%

“…Additionally, G was negatively correlated with CDM, but positively correlated with SRR and RDMY ( Table 5). The dry matter accumulation in the early stages of the plant cycle is essential for the crop because seed germination is followed by mobilization of cotyledon reserves, which contain the necessary energy for growth until seedlings become photoautotrophic (Pritchard et al, 2002). Table 5.…”

Section: Resultsmentioning

confidence: 99%

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Reserve mobilization in soybean seeds under water restriction after storage

et al. 2020

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Germination does not guarantee plant establishment if there is no efficient and enough reserve mobilization from seeds to the initial seedling growth, mainly in adverse situations. Therefore, this study aimed to evaluate the mobilization of reserves in soybean seeds under water restriction. Four soybean seed lots previously stored for one year were used in the study. To simulate water stress, we used polyethylene glycol (PEG) solutions at concentrations of 0.0, -0.1, and -0.2 MPa. The number of normal, infected and abnormal seedlings were evaluated, along with the number of dead and hard seeds. The radicle, hypocotyl, and cotyledons were separated to determine the dry weight based on the total seed reserve available for mobilization. From this value, the seed reserve reduction, relative dry matter yield, seed reserve reduction rate, and reserve conversion efficiency into dry seedling weight were determined. The increase in water restriction elevates the reserve consumption during the germination process. Vigorous seeds are more efficient in mobilizing and converting grains reserve in dry seedling mass. In less vigorous seeds, mainly those on water restriction, the increase in reserve consumption does not result in conversion into dry seedling mass.

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“…The values of carbon dioxide obtained in the readings were later divided by the weight of the seeds present in the tube and by the time that remained in the tube until the reading, in this case 24 h, being the results expressed as % CO 2 .g -1 .h -1 . As a calibration measure, a blank test was performed with four Falcons tubes containing only the moistened blotting paper sheet without the seeds (Santos et al, 2016).…”

Section: Methodsmentioning

confidence: 99%

Adequacy of the tetrazolium test methodology for Stylosanthes capitata Vogel seeds

et al. 2018

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The seed sector is increasingly dynamic and therefore demands rapid decision-making. The tetrazolium test is a rapid test that determines the viability of seeds, especially from species that show slow germination or dormancy. The methodology described in the Rules for Seed Testing for Stylosanthes sp. is not applied effectively to the Stylosanthes capitata Vogel species. Three seed lots of S. capitata seeds with different quality levels were used to fit the tetrazolium test methodology. In order to determine the physiological quality of seeds, the tests of germination, first count germination, emergence speed index, initial and final seedling emergence percentage, electrical conductivity, respiratory activity and seed moisture content were performed, besides the imbibition curve for the establishment of pre-imbibition periods of the seeds to perform the tetrazolium test. The pre-imbibition and staining periods of 6, 12 and 18 h, and 3, 6, 12 and 24 h at concentrations of 0.075%, 0.1% and 0.5% tetrazolium solution were tested. It was concluded that the 12-h pre-imbibition period and 6-h staining at the 0.1% tetrazolium solution is ideal for evaluating the viability of S. capitata seeds.Index terms: forage seeds, viability, vigor.Adequação da metodologia do teste de tetrazólio em sementes de Stylosanthes capitata Vogel RESUMO -O setor sementeiro está cada vez mais dinâmico e por isso demanda rápidas tomadas de decisões. O teste de tetrazólio é um teste rápido que determina a viabilidade das sementes, principalmente de espécies que apresentam germinação lenta ou dormência. A metodologia contida nas Regras para Análise de Sementes para a espécie Stylosanthes sp. não se aplica efetivamente para a espécie Stylosanthes capitata Vogel. Para adequar a metodologia de condução do teste de tetrazólio, foram utilizados três lotes de sementes de S. capitata com níveis de qualidade distintos. Na determinação da qualidade fisiológica das sementes, foram realizados os testes de germinação, primeira contagem da germinação, índice de velocidade de emergência, estandes inicial e final, condutividade elétrica, atividade respiratória e grau de umidade, além da curva de embebição para estabelecimento dos períodos de pré-embebição das sementes para a condução do teste de tetrazólio. Foram testados os períodos de pré-embebição e coloração, de 6, 12 e 18 h e, 3, 6, 12 e 24 h, nas concentrações de 0,075%, 0,1% e 0,5% da solução de tetrazólio. Concluiu-se que o período de 12 h de pré-embebição e 6 h de coloração na solução de tetrazólio a 0,1% é o ideal para a avalição da viabilidade de sementes de S. capitata.Termos para indexação: sementes forrageiras, viabilidade, vigor. 1The 12 h period of pre-imbibition and 6 h staining at 0.1% concentration of the tetrazolium solution is ideal to evaluate the viability of Stylosanthes capitata seeds. Tetrazolium test on Stylosanthes seeds

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Physiological quality of hybrid maize seeds through respiratory and enzymatic activities

Cited by 7 publications

References 16 publications

Respiratory Activity as a Fast Method for Vigor Differentiation of Habanero Pepper Seeds

Respiratory Activity as a Fast Method for Vigor Differentiation of Habanero Pepper Seeds

Reserve mobilization in soybean seeds under water restriction after storage

Adequacy of the tetrazolium test methodology for Stylosanthes capitata Vogel seeds

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