Physiological Markers for Lily Bulb Maturity

Gude, H.; Verbruggen, J.J.M.; Franssen, J.M.; Schouten, E.T.J.; Aanholt, J.T.M. van; Boonekamp, P.M.

doi:10.17660/actahortic.2000.517.43

Cited by 7 publications

(5 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The formation and development of Lilium bulblets is closely related with carbohydrate metabolism, 26,27) especially of starch and sucrose. 28) In this study, starch and sucrose contents were determined during the preparation of regenerated bulblets in vitro.…”

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and direct as well as indirect organogenesis inLilium pumilumDC. Fisch., an endangered ornamental and medicinal plant

Zhang

Gai

et al. 2016

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

Somatic embryogenesis and organogenesis in Lilium pumilum were successfully regulated by picloram, α-naphthaleneacetic acid (NAA), and 6-benzyladenine (BA). In organogenesis, the highest shoot regeneration frequency (92.5%) was obtained directly from bulb scales on Murashige and Skoog (MS) medium containing 2.0 mg L(-1) BA and 0.2 mg L(-1) NAA, while organogenic callus (OC) formed from leaves on MS medium supplemented with 1.0 mg L(-1) BA and 0.5 mg L(-1) NAA. Following subculture, 76.7% of OC regenerated shoots. In somatic embryogenesis, the combination of picloram and NAA increased the amount of embryogenic callus (EC) that formed with a maximum on 90.7% of all explants which formed 11 somatic embryos (SEs) per explant. Differences between EC and OC in cellular morphology and cell differentiation fate were easily observed. SEs initially formed via an exogenous or an endogenous origin. The appearance of a protoderm in heart-shaped SE and the bipolar shoot-root development in oval-shaped SE indicated true somatic embryogenesis. This protocol provides a new and detailed regulation and histological examination of regeneration pattern in L. pumilum.

show abstract

Section: Discussionmentioning

confidence: 99%

Somatic embryogenesis and direct as well as indirect organogenesis inLilium pumilumDC. Fisch., an endangered ornamental and medicinal plant

Zhang

Gai

et al. 2016

Bioscience, Biotechnology, and Biochemistry

View full text Add to dashboard Cite

show abstract

“…Sucrose has been shown to be the main soluble carbohydrate associated with bulb development (Gude and Verbruggen, 2000;Shin et al, 2002). More than half the total soluble sugars detected at the various growth stages was sucrose ( Table 1).…”

Section: Behavior Of Carbohydrates In Lily Bulbsmentioning

confidence: 99%

Studies on the Bulb Development and Its Physiological Mechanisms in Lilium Oriental Hybrids

Xia¹,

Zheng²,

Huang³

2005

Acta Hortic.

View full text Add to dashboard Cite

Based on the growth observation of lily bulbs (Lilium Oriental hybrids, 'Acapulco', 'Marco Polo', 'Siberia', 'Sobonne' and 'Tiber'), we found the mountainous condition was suitable for the bulb development. Total 288,020 bulbs (12-14 cm, 14-16 cm and 16-18 cm in circumference) of Oriental lily were harvested from 401,500 imported bulblets (6/9 cm), which were grown in the mountainous region (Jinyun, Zhejiang Province) having an elevation of 880 m. The average ratio for harvested flowering bulbs (>12 cm) and big bulbs (16-18 cm) was 71.7% and 15.4%, respectively. The concentrations of soluble sugar, sucrose and starch in the bulb scales, as well as leaf photosynthesis were investigated during the whole year cultivation. It was found that the fresh weight and circumference of harvested bulbs have the positive correlation between vegetative growth in lily. The bud removal improved the process of bulb development. Accumulation and transportation of carbohydrates in the bulbs also increased significantly from the bud-removal. The average concentrations of starch and total soluble sugar were 293 mg g FW-1 and 9.2 mg g FW-1 in the harvested bulbs, respectively. It was indicated that the quality of mountainous reproduction bulbs was similar to that of the commercial bulbs from Holland.

show abstract

“…The effects and scope of low temperature change greatly depend on species or varieties [1]. Proper chilling treatments (0–8 °C) are required for at least 6–8 weeks to break the bulb’s dormancy for the majority of Oriental hybrid lilies [2]. Some Asiatic hybrids only need 4 weeks to release bulbs’ dormancy [3].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional Regulatory Network of GA Floral Induction Pathway in LA Hybrid Lily

Yong

Zhang

et al. 2019

IJMS

View full text Add to dashboard Cite

Background: The LA hybrid lily ‘Aladdin’ has both excellent traits of Longiflorum hybrids and Asiatic hybrids—such as big and vivid flower, strong stem, high self-propagation coefficient, and shorter low temperature time required to release bulb dormancy in contrast to Oriental hybrids. A genome-wide transcriptional analysis using transcriptome RNA-Seq was performed in order to explore whether there is a gibberellin floral induction pathway in the LA hybrid lily. Subsequently, gene co-expression network analysis was used to analyze the possible interactions of key candidate genes screened from transcriptome data. At the same time, a series of physiological, biochemical, and cultivation tests were carried out. Results: The content of five endogenous hormones changed sharply in the shoot apex during the treatment of 200 mg/L exogenous gibberellin and the ratio of ABA/GA3 dropped and stayed at a lower level after 4 hours’ treatment from the higher levels initially, reaching a dynamic balance. In addition, the metabolism of carbohydrates in the bulbs increase during exogenous gibberellin treatment. A total of 124,041 unigenes were obtained by RNA-seq. With the transcriptome analysis, 48,927 unigenes and 48,725 unigenes respectively aligned to the NR database and the Uniprot database. 114,138 unigenes, 25,369 unigenes, and 19,704 unigenes respectively aligned to the COG, GO, and KEGG databases. 2148 differentially expression genes (DEGs) were selected with the indicators RPKM ≥ 0, FDR ≤ 0.05 and |log2(ratio)| ≥ 2. The number of the upregulated unigenes was significantly more than the number of the downregulated unigenes. Some MADS-box genes related to flowering transformation—such as AGL20, SOC1, and CO—were found to be upregulated. A large number of gibberellin biosynthesis related genes such as GA2ox, GA3ox, GA20ox, Cytochrome P450, CYP81, and gibberellin signal transduction genes such as DELLA, GASA, and GID1 were significantly differentially expressed. The plant hormones related genes such as NCED3 and sugar metabolism related genes such as α-amylase, sucrose synthase hexokinase, and so on were also found expressing differentially. In addition, stress resistance related genes such as LEA1, LEA2, LEA4, serine/threonine protein kinase, LRR receptor-like serine/threonine protein kinase, P34 kinase, histidine kinase 3 and epigenetic related genes in DNA methylation, histone methylation, acetylation, ubiquitination of ribose were also found. Particularly, a large number of transcription factors responsive to the exogenous gibberellin signal including WRKY40, WRKY33, WRKY27, WRKY21, WRKY7, MYB, AP2/EREBP, bHLH, NAC1, NAC2, and NAC11 were found to be specially expressing. 30 gene sequences were selected from a large number of differentially expressed candidate genes for qRT-PCR expression verification (0, 2, 4, 8, and 16 h) and compared with the transcriptome expression levels. Conclusions: 200mg/L exogenous GA3 can successfully break the bulb’s dormancy of the LA hybrid lily and significantly accelerated the flowering process, indicating that gibberellin floral induction pathway is present in the LA lily ‘Aladdin’. With the GCNs analysis, two second messenger G protein-coupled receptor related genes that respond to gibberellin signals in the cell were discovered. The downstream transport proteins such as AMT, calcium transport ATPase, and plasma membrane ATPase were also discovered participating in GA signal transduction. Transcription factors including WRKY7, NAC2, NAC11, and CBF specially regulated phosphorylation and glycosylation during the ubiquitination degradation process of DELLA proteins. These transcription factors also activated in abscisic acid metabolism. A large number of transcription factors such as WRKY21, WRKY22, NAC1, AP2, EREB1, P450, and CYP81 that both regulate gibberellin signaling and low-temperature signals have also been found. Finally, the molecular mechanism of GA floral induction pathway in the LA hybrid lily ‘Aladdin’ was constructed.

show abstract

Physiological Markers for Lily Bulb Maturity

Cited by 7 publications

References 0 publications

Somatic embryogenesis and direct as well as indirect organogenesis inLilium pumilumDC. Fisch., an endangered ornamental and medicinal plant

Somatic embryogenesis and direct as well as indirect organogenesis inLilium pumilumDC. Fisch., an endangered ornamental and medicinal plant

Studies on the Bulb Development and Its Physiological Mechanisms in Lilium Oriental Hybrids

Transcriptional Regulatory Network of GA Floral Induction Pathway in LA Hybrid Lily

Contact Info

Product

Resources

About