Physiological effects of narciclasine from the mucilage of Narcissus tazetta L. bulbs

Bi, Yurong; Yung, Kung-Hing; Wong, Yum-Shing

doi:10.1016/s0168-9452(98)00069-7

Cited by 30 publications

(31 citation statements)

References 7 publications

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“…The mean IC 50 of ∼50 nmol/L calculated on the 6 human glioblastoma multiforme was similar to the value we previously reported for 6 carcinoma cell lines (14) and consistent with National Cancer Institute data, which also reveal a mean IC 50 value of 47 nmol/L for the compound across a panel of 60 cancer cell lines. 8 We recently showed that the in vitro growth-inhibitory activity of high narciclasine concentrations (>1 μmol/L) in carcinoma cells relates to proapoptotic effects resulting from compound-mediated triggering of the activation of the initiator caspases of the death receptor pathway (14). In the present study, we failed to show any proapoptotic effects for narciclasine in U373 (data not shown), Hs683 (data not shown), and GL19 (Fig.…”

Section: Resultscontrasting

confidence: 46%

“…Narciclasine isolated from Narcissus bulbs has been shown to be a plant growth inhibitor (8). Actin filaments are the main players in plant cell and pollen grain growth (43).…”

Section: Molecular Cancer Therapeuticsmentioning

confidence: 99%

“…Narciclasine is a plant growth inhibitor isolated from Narcissus bulbs (8). It has been shown that narciclasine exhibits a wide range of inhibitory effects on plant growth including seed germination, seedling growth, growth and plastid development of excised radish cotyledons (inhibiting the transition of glyoxysomes and peroxisomes), and chloroplast development (9).…”

Section: Introductionmentioning

confidence: 99%

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Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells

Lefranc

Sauvage

Goietsenoven

et al. 2009

Molecular Cancer Therapeutics

107

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Cell motility and resistance to apoptosis characterize glioblastoma multiforme growth and malignancy. Narciclasine, a plant growth modulator, could represent a powerful new weapon targeting the Achilles' heel of glioblastoma multiforme and may offer the potential to better combat these devastating malignancies. The in vitro effects of narciclasine on cell proliferation, morphology, actin cytoskeleton organization, and the Rho/Rho kinase/LIM kinase/cofilin pathway and its antitumor activity in vivo have been determined in models of human glioblastoma multiforme. Narciclasine impairs glioblastoma multiforme growth by markedly decreasing mitotic rates without inducing apoptosis. The compound also modulates the Rho/Rho kinase/LIM kinase/cofilin signaling pathway, greatly increasing GTPase RhoA activity as well as inducing actin stress fiber formation in a RhoA-dependent manner. Lastly, the treatment of human glioblastoma multiforme orthotopic xenograft-bearing mice with nontoxic doses of narciclasine significantly increased their survival.Narciclasine antitumor effects were of the same magnitude as those of temozolomide, the drug associated with the highest therapeutic benefits in treating glioblastoma multiforme patients. Our results show for the first time that narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma multiforme cells and significantly increases the survival of human glioblastoma multiforme preclinical models. This statement is made despite the recognition that to date, irrespective of treatment, no single glioblastoma multiforme patient has been cured.

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Section: Resultscontrasting

confidence: 46%

“…Narciclasine isolated from Narcissus bulbs has been shown to be a plant growth inhibitor (8). Actin filaments are the main players in plant cell and pollen grain growth (43).…”

Section: Molecular Cancer Therapeuticsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells

Lefranc

Sauvage

Goietsenoven

et al. 2009

Molecular Cancer Therapeutics

107

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“…The mode of action of NCS on the susceptible plant cells is not known although it has been shown to inhibit protein synthesis (Carrasco et al 1975) and exhibit antimitotic (Ceriotti 1967) and antiviral activity (Gabrielsen et al 1992). Our previous study (Bi et al 1998) indicated that NCS inhibited the growth of excised radish cotyledons. If seedlings, especially those of the family Poaceae including members such as wheat and barley, are grown in darkness, the plastids develop into etioplasts.…”

Section: Discussionmentioning

confidence: 99%

“…It has been shown that NCS exhibits a wide range of inhibitory effects on plant growth. NCS also interacts with different plant hormones in various physiological responses, such as blocking the promotion of wheat coleoptile elongation by indole-3-acetic acid (IAA), the enhancement of excised radish cotyledons by 6-benzylaminopurine (BA), and oc-amylase activity of wheat (Triticum aestivum) seeds induced by gibberellic acid (GA) (Bi et al 1998).…”

Section: Introductionmentioning

confidence: 99%

Narciclasine alters chloroplast membrane structure and inhibits 5‐aminolevulinic acid and chlorophyll binding protein accumulation in wheat (Triticum aestivum) leaves

Zhang

Guo

et al. 2003

New Zealand Journal of Crop and Horticultural Science

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Narciclasine (NCS), an Amaryllidaceae alkaloid from Narcissus tazetta bulbs, is a potent plant growth inhibitor. It shows a broad range of inhibitory effects on seed germination and seedling growth in wheat (Triticum aestivum). In this study, the chlorophyll content of light-grown wheat seedlings was markedly reduced in the presence of NCS. In etiolated wheat leaf sections, the greening process initiated upon illumination was inhibited by NCS. The block of chlorophyll accumulation in the presence of NCS was most probably due to the block of the formation of 5-aminolevulinic acid, an essential chlorophyll precursor. Western blot analysis showed that the formation of light harvesting chlorophyll a/b binding protein (LHCII) was also inhibited by NCS. Electron microscopy studies provided direct observation that the formation of chloroplast and internal membrane † Corresponding author. systems were blocked when etioplasts were illuminated in the presence of NCS. Thus, our study showed that NCS inhibited chlorophyll and chloroplast protein accumulation, concomitantly chloroplast formation.

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Solid‐phase extraction and reversed‐phase high‐performance liquid chromatography of the five major alkaloids in Narcissus confusus

López

Viladomat

Codina

2002

Phytochemical Analysis

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A novel, fast and precise method, combining solid-phase extraction and reversed-phase high-performance liquid chromatography is described for the quantitative determination of five alkaloids (galanthamine, N-formylnorgalanthamine, haemanthamine, homolycorine and tazettine/pretazettine) from bulbs of wild Narcissus confusus, a high galanthamine-containing plant species growing in the Iberian Peninsula.

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Physiological effects of narciclasine from the mucilage of Narcissus tazetta L. bulbs

Cited by 30 publications

References 7 publications

Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells

Narciclasine, a plant growth modulator, activates Rho and stress fibers in glioblastoma cells

Narciclasine alters chloroplast membrane structure and inhibits 5‐aminolevulinic acid and chlorophyll binding protein accumulation in wheat (Triticum aestivum) leaves

Solid‐phase extraction and reversed‐phase high‐performance liquid chromatography of the five major alkaloids in Narcissus confusus

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