Solid‐phase extraction and reversed‐phase high‐performance liquid chromatography of the five major alkaloids in Narcissus confusus

Abstract

A novel, fast and precise method, combining solid-phase extraction and reversed-phase high-performance liquid chromatography is described for the quantitative determination of five alkaloids (galanthamine, N-formylnorgalanthamine, haemanthamine, homolycorine and tazettine/pretazettine) from bulbs of wild Narcissus confusus, a high galanthamine-containing plant species growing in the Iberian Peninsula.

Analysis of Amaryllidaceae alkaloids from Narcissus by GC–MS and capillary electrophoresis

“…In the present paper capillary electrophoresis is proposed as a useful alternative to the existing HPLC method [11] for the determination of galanthamine and haemanthamine in Amaryllidaceae extracts; in particular, the association of CE with GC-MS was found to be well suitable for identification of the studied alkaloids in bulbs of two different species of ornamental Narcissus. The usefulness of the proposed approaches lies in the orthogonal selectivity of GC and CE; in fact different separation profiles are expected to be obtained for a given plant extract analysed with the two techniques.…”

“…In these studies TLC [8,9], GC-MS [8][9][10] and HPLC [11,12] were successfully applied; in particular GC-MS was found to be suitable for the characterization of the alkaloids. The power of GC-MS has been exploited in these works, especially because the main Amaryllidaceae alkaloids can be easily subjected to direct GC analysis without any derivatization step.…”

Pressurized liquid extraction and anticholinesterase activity-based thin-layer chromatography with bioautography of Amaryllidaceae alkaloids

“…Obtained purified extracts showed much lower background absorbance both in densitometry and RP- HPLC-DAD, which is normally less common when SPE omitting cation-exchange sorbent (i.e. on C 18 [20]) used.…”

“…But this process is rather time-and solventconsuming with poor efficiency. On the other hand, solid-phase extraction (SPE) on C 18 RP cartridges was sometimes used [20]. However, methanolic-water extracts thereafter obtained, contain usually polar co-extractives, which influence the separation and background absorbance in HPLC.…”

Improved HPLC Metod for the Determination of Amaryllidaceae Alkaloids

“…HPLC methods were used for determination of some Amaryllidaceae alkaloids in plant and tissue-culture extracts of Narcissus ssp. (11,12,18) and Leucojum aestivum L. (11,14,16). These methods differ mainly in the column type, pH range of the mobile phase, and analysed plants.…”