Physiological effects and metabolism of gossypol

Abou‐Donia, Mohamed B.

doi:10.1007/978-1-4613-9401-3_5

Cited by 124 publications

(109 citation statements)

References 134 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nos ruminantes a molécula de gossipol não é metabolizada pelas bactérias do rúmen nem pelo animal (Abou-Donia 1976). Ela se une às proteínas que contém aminoácidos livres, impedindo seu metabolismo (Reiser 1962).…”

Section: Introductionunclassified

Mecanismos da intoxicação do fígado de rato causada pelo gossipol

Carvalho

Maioli

Mendes³

et al. 2013

Pesq. Vet. Bras.

View full text Add to dashboard Cite

The liver plays a central role in metabolism due to its interposition between the digestive tract and the general circulation of the organism. It is also the main organ involved in biotransformation of exogenous substances (xenobiotics), with ability to convert hydrophobic compounds in water-soluble, more easily eliminated by the body. Gossypol is a toxic phenolic substance present in cotton seed (Gossypium sp.). Aiming to study the mechanisms involved in the hepatotoxicity of gossypol we evaluate its effects on the antioxidant system of rat liver performing an experiment that investigated the oxidative stress and the histopathological alterations. In this study, we used Wistar rats, divided into two groups, one that received canola oil (vehicle, Control group) and another that received gossypol at a dose of 40mg/kg body weight of the animal for 15 days (Treated group). The treatment with gossypol caused alterations in the activity of seric enzymes that indicate hepatic injury and a significant oxidative stress characterized by a decrease of reduced glutathione (GSH) levels and a consequent increase in oxidized glutathione (GSSG), including further damage to the plasma membrane and organelles showed by lipid peroxidation. The result of histopathological evaluation showed degeneration of the hepatocytes.

show abstract

Section: Introductionunclassified

Mecanismos da intoxicação do fígado de rato causada pelo gossipol

Carvalho

Maioli

Mendes³

et al. 2013

Pesq. Vet. Bras.

View full text Add to dashboard Cite

show abstract

“…1) (Adams et al, 1960;Abou-Donia, 1976). It exists in three tautomeric forms: aldehyde, ketone, and hemiacetal.…”

Section: Gossypolmentioning

confidence: 99%

Effects of gossypol on the motility of mammalian sperm

Porat

1990

Molecular Reproduction Devel

View full text Add to dashboard Cite

show abstract

“…2 It is undesirable for different animal species, especially for poultry and swine. 3 Several procedures have been described in the literature for the analysis of gossypol, including procedures based on high performance liquid chromatography (HPLC), 4,5 enzyme-linked immunosorbent assay 6 and Fourier transform infrared spectroscopy (FTIR). 7 The spectrophotometric determination of gossypol can be performed using 3-amino-1-propanol and its subsequent complexation with iron(III), forming a green-colored complex with maximum absorption at 620 nm.…”

Section: Introductionmentioning

confidence: 99%

“…A linear response was observed from 0.96 to 3.85 mmol L -1 , and the detection limit, the coefficient of variation and the sampling rate were estimated at 100 µmol L -1 (99.7% confidence level), 1.90% (n = 20) and 30 h -1 , respectively. The reagent consumption was 1.08 mg of Fe(NO 3 ) 3 , with a volume of 20 mL of effluent per determination. Analyses of the results of commercial samples were consistent with through the official procedure of detoxification of cottonseed meal as described by American Oil Chemists' Society (AOCS) at the 95% confidence level.…”

mentioning

confidence: 99%

An Environmental Friendly Flow-Injection Procedure for On Line Extraction and Spectrophotometric Determination of Gossypol in Cottonseed Meal

Daminato

Kamogawa

Melchert

2017

J. Braz. Chem. Soc.

View full text Add to dashboard Cite

A fast, simple and inexpensive procedure is described for the determination of gossypol in cottonseed meal. The procedure is based on a flow injection analysis system and a complexation reaction with spectrophotometric detection at 620 nm. A fluidized bed column was used to implement the on line that was coupled to the extraction flow system in order to analyze the solid samples directly. A linear response was observed from 0.96 to 3.85 mmol L -1 , and the detection limit, the coefficient of variation and the sampling rate were estimated at 100 µmol L -1 (99.7% confidence level), 1.90% (n = 20) and 30 h -1 , respectively. The reagent consumption was 1.08 mg of Fe(NO 3 ) 3 , with a volume of 20 mL of effluent per determination. Analyses of the results of commercial samples were consistent with through the official procedure of detoxification of cottonseed meal as described by American Oil Chemists' Society (AOCS) at the 95% confidence level. Keywords: gossypol, flow analysis, spectrophotometric detection IntroductionCottonseed meal is obtained in the oil extraction process. It is high in protein, fiber and minerals and can be used as a fertilizer and animal feed. However, its use is limited by the presence of the polyphenolic yellow compound known as gossypol.1 Gossypol (2,2'-bis(8-formyl-1,6,7-trihydroxy-5-isopropyl-3-methylnaphthalene) is a toxic pigment that occurs in various parts of cotton plants. 2 It is undesirable for different animal species, especially for poultry and swine. 3 Several procedures have been described in the literature for the analysis of gossypol, including procedures based on high performance liquid chromatography (HPLC), 4,5 enzyme-linked immunosorbent assay 6 and Fourier transform infrared spectroscopy (FTIR). 7 The spectrophotometric determination of gossypol can be performed using 3-amino-1-propanol and its subsequent complexation with iron(III), forming a green-colored complex with maximum absorption at 620 nm. To extract the analyte from the cottonseeds, 80 mL of hexane-isopropyl alcohol is used per determination.2 Another procedure is based on secondderivative ultraviolet spectrophotometry and it involves the extraction of free gossypol using aqueous acetone, hydrolysis of the soluble-bound forms with hydrochloric acid and the partitioning of the pure compound into chloroform. The proposed method showed good precision (4.0%), but a large amount of organic solvents was used (50 mL acetone and 50 mL chloroform) and a lot of time was spent (2 h) on each sample.8 These drawbacks hinder the use of these procedures for routine analysis.Flow-injection systems (FIA) have been employed especially for the mechanization of analytical procedures in order to minimize the analyst intervention and enhance the number of samples processed by time and improving precision. In the preparation of samples, FIA can be used to minimize risks of sample contamination and amounts of organic solvents, as well as reduce costs of analysis. These systems also show great potential for the development of greener ...

show abstract

Physiological effects and metabolism of gossypol

Cited by 124 publications

References 134 publications

Mecanismos da intoxicação do fígado de rato causada pelo gossipol

Mecanismos da intoxicação do fígado de rato causada pelo gossipol

Effects of gossypol on the motility of mammalian sperm

An Environmental Friendly Flow-Injection Procedure for On Line Extraction and Spectrophotometric Determination of Gossypol in Cottonseed Meal

Contact Info

Product

Resources

About