Effects of gossypol on the motility of mammalian sperm

Porat, Offie

doi:10.1002/mrd.1080250414

Cited by 20 publications

(20 citation statements)

References 108 publications

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“…The adverse effects of SAL on the testis are comparable with that of polyphenols like gossypol. Similar dysfunctions in the testis including decreased weight, regressed seminiferous tubules, reduced number of spermatozoa with reduced motility were reported in many mammalian species following gossypol administration [52–54]. The vacuolated epithelium of epididymis and aggregation of necrotic cells in its lumen indicated abnormal spermatogenesis as well as lack of testosterone supply [55].…”

Section: Discussionmentioning

confidence: 61%

Dose-Dependent Adverse Effects of Salinomycin on Male Reproductive Organs and Fertility in Mice

2013

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Salinomycin is used as an antibiotic in animal husbandry. Its implication in cancer therapy has recently been proposed. Present study evaluated the toxic effects of Salinomycin on male reproductive system of mice. Doses of 1, 3 or 5 mg/kg of Salinomycin were administered daily for 28 days. Half of the mice were sacrificed after 24 h of the last treatment and other half were sacrificed 28 days after withdrawal of treatment. Effects of SAL on body and reproductive organ weights were studied. Histoarchitecture of testis and epididymis was evaluated along with ultrastructural changes in Leydig cells. Serum and testicular testosterone and luteinizing hormones were estimated. Superoxide dismutase, reduced glutathione, lipid peroxidation, catalase and lactate dehydrogenase activities were measured. Spermatozoa count, morphology, motility and fertility were evaluated. Expression patterns of steroidogenic acute regulatory protein (StAR) and cytochrome P450 side chain cleavage proteins (CYP11A1) were assessed by Western blotting. Salinomycin treatment was lethal to few mice and retarded body growth in others with decreased weight of testes and seminal vesicles in a dose dependent manner. Seminiferous tubules in testes were disrupted and the epithelium of epididymis showed frequent occurrence of vacuolization and necrosis. Leydig cells showed hypertrophied cytoplasm with shrunken nuclei, condensed mitochondria, proliferated endoplasmic reticulum and increased number of lipid droplets. Salinomycin decreased motility and spermatozoa count with increased number of abnormal spermatozoa leading to infertility. The testosterone and luteinizing hormone levels were decreased in testis but increased in serum at higher doses. Depletion of superoxide dismutase and reduced glutathione with increased lipid peroxidation in both testis and epididymis indicated generation of oxidative stress. Suppressed expression of StAR and CYP11A1 proteins indicates inhibition of steroidogenesis. Spermatogenesis was however observed in testis 28 days after Salinomycin withdrawal. The results indicate reversible dose-dependent adverse effects of Salinomycin on male reproductive system of mice.

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Section: Discussionmentioning

confidence: 61%

Dose-Dependent Adverse Effects of Salinomycin on Male Reproductive Organs and Fertility in Mice

2013

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“…It has been used traditionally to treat bronchitis and to induce abortion and menses, but was only recognized as a male antifertility agent in the 1970s. [40] Whether infertility induced by gossypol is reversible is in dispute. GOS is toxic to non-ruminant animals and has limited the use of cottonseed meal as a dietary source of proteins for farm animals.…”

Section: Discussionmentioning

confidence: 99%

“…[28] Studies on the mechanism of action of gossypol suggest structural alterations of cell membranes, inhibition of enzymes and energy metabolism may be affected. [40] The effect of GOS on spermatogenesis results in the arrest of spermatozoa development and finally sterilization of male. [50] Deleterious effects of GOS toward spermatozoa include immobilization of spermatozoa motility and fertilizing ability.…”

Section: Discussionmentioning

confidence: 99%

“…[48] As a potential male antifertility agent GOS is believed to be spermicidal-it inhibits spermatozoa motility, and causes azoospermia, at different doses and with marked species variability and inhibits spermatogenesis. [40] The oral administration of 20 mg/day GOS for 75 days was found to be highly effective in suppressing spermatogenesis to a level as low as 4 million spermatozoa per mL and its efficacy was reported at 98.5% in 8,806 male volunteers in China. [49] Damage to both spermatozoa morphology and spermatogenesis can be induced in the pubertal bovine male by feeding of cottonseed meal containing relatively high levels (3000 ppm) of GOS.…”

Section: Discussionmentioning

confidence: 99%

“…[34] No suitable animal model for studies on GOS, either in vivo or in vitro is available. [40] Now-a-days increased attention is given to the use of in vitro toxicity testing due to public concerns related to animal welfare as well as other benefits offered by this approach. The objectives of the present study were to: 1) examine the in vitro dose response of rabbit spermatozoa motility to GOS and 2) determine whether filtered (FIL) and unfiltered (UNFIL) gossypol differ in their magnitude of effect.…”

Section: Introductionmentioning

confidence: 99%

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In vitrogossypol induced spermatozoa motility alterations in rabbits

Roychoudhury

Massányi

Slamečka³

et al. 2009

Journal of Environmental Science and Health, Part B

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The objectives of the present study were to: (i) examine the in vitro dose response of rabbit spermatozoa motility to the antifertility agent gossypol (GOS) and (ii) determine whether filtered (FIL) and unfiltered (UNFIL) GOS differ in their magnitude of effect. Rabbit semen belonging to adult males (n = 5; 12-14 months) were cultured with UNFIL GOS and FIL GOS (5% solution) and subsequently diluted (1:1-7) for analysis using a Computer Assisted Semen Analyzer (CASA) system in 5 time periods (0, 60, 120, 180 and 360 minutes). At Time 0, no significant change in rabbit spermatozoa motility (MOT) and progressive motility (PROG) with GOS FIL was noted, while increases were observed with GOS UNFIL. At Time 60, weak changes were noted for MOT and PROG. After 120 minutes of culture with both GOS FIL and GOS UNFIL, MOT and PROG decreased significantly in some experimental groups. However, no differences were recorded for both the parameters at Times 180 and 360, with the exception of PROG in the GOS UNFIL category (groups A, B, E, F and G), where a significant decrease was noticed. Detailed evaluation of the distance and velocity parameters revealed reduction in all these studied markers after 60 and 120 minutes of in vitro culture with both GOS FIL and GOS UNFIL, indirectly confirming the PROG decrease. Straightness (STR), linearity (LIN), wobble (WOB), amplitude of lateral head displacement (ALH) and beat cross frequency (BCF) mostly remained unaltered at all time periods for GOS FIL, where as some minor alterations were noticed in GOS UNFIL category for STR, LIN, WOB, ALH and BCF parameters at Time 0, 60 and 120. The present study confirms the dose and time dependent alterations of rabbit spermatozoa motility parameters by GOS. The GOS dynamics in our experiment shows that rabbit spermatozoa as a biological material can indicate a GOS inhibition of motility. Obtained data for the first time indicates a higher immobilizing potential of unfiltered GOS in comparison to filtered GOS in its inhibitory action of spermatozoa motility parameters in rabbits.

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Effect of Azorellanone, a Diterpene FromAzorella yaretaHauman, on Human Sperm Physiology

Morales¹,

Kong²,

Pizarro³

et al. 2003

Journal of Andrology

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Previous studies have shown that cyclic terpenes extracted from plants decrease sperm motility and concentration in rats. In this work, we studied the effect 13-alpha-hydroxy-7-oxoazorellano (azorellanone), a cyclic diterpene extracted from Azorella yareta Hauman, on in vitro human sperm physiology. Sperm aliquots, capacitated for 4.5 or 20 hours, were incubated for 15 minutes with different concentrations of azorellanone. Then, the effects of azorellanone on sperm motility, viability, binding to the human zona pellucida, progesterone-induced acrosome reactions and increase in intracellular Ca(2)(+) concentration, and trypsin and chymotrypsin-like protease activities were evaluated. Sperm motility was evaluated according to World Health Organization (WHO) guidelines; sperm viability with the supravital dye Hoescht 33258; sperm-zona binding with the hemizona assay; progesterone-induced acrosome reaction with fluorescent lectin; intracellular Ca(2)(+) level with fura 2; and protease activity with the synthetic substrates N-t-Boc-Gln-Ala-Arg-Amido-4-methylcoumaryn and Succinyl-Leu-Leu-Val-Tyr-Amido-4-methylcoumaryn. The results obtained indicate that azorellanone inhibited sperm motility in a concentration-dependent manner at 0.15, 1.5, and 3 mM, while sperm viability was only inhibited at 3 mM. Treatment with azorellanone significantly inhibited sperm-zona binding, progesterone-induced acrosome reactions, and intracellular Ca(2)(+) concentration. Treatment of free-swimming sperm with azorellanone did not affect protease activity; however, the incubation of sperm extracts with azorellanone significantly inhibited both trypsin-like and chymotrypsin-like protease activities. In conclusion, azorellanone has a significant effect on the different parameters that characterize human sperm physiology.

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Effects of gossypol on the motility of mammalian sperm

Cited by 20 publications

References 108 publications

Dose-Dependent Adverse Effects of Salinomycin on Male Reproductive Organs and Fertility in Mice

Dose-Dependent Adverse Effects of Salinomycin on Male Reproductive Organs and Fertility in Mice

In vitrogossypol induced spermatozoa motility alterations in rabbits

Effect of Azorellanone, a Diterpene FromAzorella yaretaHauman, on Human Sperm Physiology

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