Physiological consequences of gamma ray irradiation in tall fescue with elimination potential of Epichloë fungal endophyte

Amirikhah, Rahim; Etemadi, Nematollah; Rahimmalek, Mehdi; Nikbakht, Ali; Eskandari, Ali

doi:10.1016/j.ecoenv.2019.109412

Cited by 6 publications

(4 citation statements)

References 47 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Flowchart for targeted screening of cold-tolerant mutants and evaluation of cold-tolerance in Plumbago indica L. Li et al 10.3389/fpls.2023.1149669 In the field, the cold-tolerant mutants SA19 and SA24 were found to have reduced plant height, delayed flowering and short inflorescences, which are consistent with the studies of Li et al (2022c) and Li et al (2021). The phenomenon may be related to plant cell damage or DNA mutation due to mutagenesis, resulting in damage during plant growth and development, but such damage does not necessarily affect the ornamental traits of the plant (Amirikhah et al, 2019). In this study, the Plumbago indica L. cold-resistant mutant showed overall delayed flowering and delayed senescence, and was able to maintain good ornamental traits, making it a better mutant for both cold tolerance and medicinal ornamental properties.…”

Section: Figure 17supporting

confidence: 61%

“…(2021) . The phenomenon may be related to plant cell damage or DNA mutation due to mutagenesis, resulting in damage during plant growth and development, but such damage does not necessarily affect the ornamental traits of the plant ( Amirikhah et al., 2019 ). In this study, the Plumbago indica L. cold-resistant mutant showed overall delayed flowering and delayed senescence, and was able to maintain good ornamental traits, making it a better mutant for both cold tolerance and medicinal ornamental properties.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

Plumbago indica L. is a perennial herb with ornamental and anticancer medicinal functions widely distributed in the tropics. It is affected by temperature and cannot bloom normally in colder subtropical regions, which seriously affects its ornamental value. To create low-temperature resistance mutants and enrich new germplasm resources, this study used tissue culture and chemical reagent (0.5 mmol/L NaN3) and low-temperature stress (0°C, full darkness for 48h) induction to target and screen for cold-resistance mutants. The results showed that the ISSR band polymorphism ratio of the 24 suspected mutant materials was 87.5%. The DNA profiles of the 9 mutants initially identified were altered. The content of plumbagin in the stems and leaves of the mutants was examined, and it was found that the accumulation in the leaves of the mutant SA24 could be as high as 3.84 times that of the control, which was 0.5991%. There were significant differences in the anatomical structures of roots, stems and leaves. The mutants mostly exhibited reduced root diameter (only 0.17-0.69 times that of CK), increased stem diameter (up to 2.19 times that of CK), enlarged mesophyll cells, increased thickness (up to 1.83 times that of CK) and high specificity, which are thought to be important for the different cold resistance obtained by the mutants. In the cold resistance experiment, four cold-tolerant mutants were successfully screened according to their morphological characteristics and physiological indexes, and the mutagenesis efficiency could be as high as 2.22% and did not affect the accumulation of plumbagin in their stems and leaves, even higher than CK. The responses of the screened mutants SA15, SA19, SA23 and SA24 to low temperature showed slower leaf wilting, higher light energy conversion efficiency, less accumulation of MDA content, increased enzymatic activities of antioxidant enzymes (SOD, CAT, POD) and more accumulation of soluble sugars and proline content. These characteristics are consistent with the response of cold-resistance plants to low temperatures. The cold- resistance mutants cultivated in soil were observed of agronomic and ornamental traits for one year, mainly manifested as delayed flowering and delayed entry into the senescence stage. This study provides a more rapid and accurate technique for identifying and screening cold-tolerant mutants, and lays the foundation for future experiments on the creation of new cold-resistant varieties.

show abstract

Section: Figure 17supporting

confidence: 61%

Section: Discussionmentioning

confidence: 99%

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Lai

et al. 2023

Front. Plant Sci.

View full text Add to dashboard Cite

show abstract

“…2 ). This is because that irradiation causes DNA breakage in plant cells, further leading to various types of damage to plant cell division and development processes, and plant growth ( Amirikhah et al, 2019 ; Li et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

“…In this study, the chlorophyll content of the tulip leaves increased at 5 Gy and then decreased with increasing irradiation. It has been reported that low doses of gamma rays (15 krad) significantly increased the total chlorophyll content of tall fescue, but high doses of gamma rays (40 krad) inhibited chlorophyll synthesis in the plants ( Amirikhah et al, 2019 ). In other words, when the levels of radiation increase above the maximum tolerable limit of the plants, the capabilities of the photosynthetic apparatus would be decreased due to the photosystem damagement, thus resulting in a decrease in chlorophyll content ( Li et al, 2021 ).…”

Section: Discussionmentioning

confidence: 99%

Biological effects of gamma-ray radiation on tulip (Tulipa gesneriana L.)

Chen

Zhan

et al. 2022

PeerJ

View full text Add to dashboard Cite

Tulip, being an important ornamental plant, generally requires lengthy and laborious procedures to develop new varieties using traditional breeding methods requires. But ionizing radiation potentially accelerates the breeding process of ornamental plant species. The biological effects of γ-ray irradiation on tulip, therefore, were investigated through establishing an irradiation-mediated mutation breeding protocol to accelerate its breeding process. ISSR-PCR molecular marker technique was further used to identify the mutants of phenotypic variation plants. This study showed that low irradiation doses (5 Gy) stimulated bulb germination to improve the survival rate of tulip, while high irradiation doses (20 to 100 Gy) significantly (P < 0.05) inhibited its seed germination and growth, and decreased the flowering rate, petal number, flower stem length and flower diameter. More than 40 Gy significantly (P < 0.05) decreased the total chlorophyll content and increased the malondialdehyde (MDA) content in tulips. Interestingly, three types of both stigma variations and flower pattern variations, and four types of flower colour variations were observed. With increasing the irradiation dose from 5 to 100 Gy, the anthocyanin and flavonoid contents continuously decreased. Scanning electron microscopy (SEM) analysis evidenced that high irradiation doses altered the micromorphology of leaf stomata. Microscopic observations of tulip root apical mitosis further showed the abnormal chromosomal division behaviour occurring at different mitotic phases under irradiation treatment (80 Gy). Increasing the irradiation dose from 20 to 100 Gy enhanced the micronucleus rate. Moreover, the suspected genetic variation in tulips was evaluated by inter-simple sequence repeat (ISSR) analysis, and the percentage of polymorphic bands was 68%. Finally, this study concludes that that 80 Gy may be an appropriate radiation does to better enhance the efficiency of mutagenic breeds in tulip plants. Using γ-ray irradiation, therefore, is expected to offer a theoretical basis for mutation breeding in tulips.

show abstract

Development of Cowpea (Vigna unguiculata) Mutant Lines for Dissecting Resilience to Drought Through Physiological and Molecular Crosstalk Analysis

Diallo,

Badiane,

Diédhiou

et al. 2024

Plant Mol Biol Rep

View full text Add to dashboard Cite

Physiological consequences of gamma ray irradiation in tall fescue with elimination potential of Epichloë fungal endophyte

Cited by 6 publications

References 47 publications

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

ISSR molecular markers and anatomical structures can assist in rapid and directional screening of cold-tolerant seedling mutants of medicinal and ornamental plant in Plumbago indica L.

Biological effects of gamma-ray radiation on tulip (Tulipa gesneriana L.)

Development of Cowpea (Vigna unguiculata) Mutant Lines for Dissecting Resilience to Drought Through Physiological and Molecular Crosstalk Analysis

Contact Info

Product

Resources

About