Biological effects of gamma-ray radiation on tulip (<i>Tulipa gesneriana</i> L.)

Li, Yirui; Chen, Li; Zhan, Xiaodie; Liu, Liang; Feng, Feihong; Guo, Zihua; Wang, Dan; Chen, Hao

doi:10.7717/peerj.12792

Cited by 18 publications

(15 citation statements)

References 58 publications

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“…The most effective primer was ISSR_21 and the worst was ISSR_62. These results indicate that the gamma-ray irradiation was the effective method to induce mutations in plants, as previously proved [2,8,14,23]. High PIC, EMR The genetic similarity based on Jaccards scores of the 'Earlibrite' strawberry mutant genotypes, ranged from 3% to 87.04%.…”

Section: Resultssupporting

confidence: 63%

Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Arisah

Saptadi²,

Ashari³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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Strawberry plantlets of the ‘Earlibrite’ variety were irradiated with gamma rays to induce genetic variability for resistance to abiotic stress. This study aimed to identify the DNA polymorphism of ‘Earlibrite’ strawberry mutants using ISSR markers. For this purpose, a population of M1V0 strawberry plants was gamma-irradiated at 20 Gy. In addition, the irradiated plantlets were subcultured onto MS medium enriched with 0.13 M of 1-NAA and 2 M of 6-BAP. The genetic variation of the mutant was molecularly analyzed using the ISSR marker. Analysis was performed on M1V0 mutant plantlets. The results showed that 93.36% of 192 scoreable bands produced by the ISSR markers were polymorphic. An average of PIC values was 0.48 per primer with a range of 0.40 (ISSR_56) to 0.50 (ISSR_22, ISSR_55, ISSR_58, ISSR_60, ISSR_61, ISSR_62 and ISSR_L10). The primer MI ranged from 2.38 (ISSR_62) to 4.85 (ISSR_21). The genetic variation of mutant individuals ranged from 2.74% to 87.04% according to the Jaccard similarity index. Cluster analysis using ISSR markers grouped the ‘Earlibrite’ strawberry mutant genotypes into five distinct groups. It is concluded that 20 Gy gamma-ray induced mutation produces strawberry mutants and that ISSR was a valuable marker for mutant identification.

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Section: Resultssupporting

confidence: 63%

Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Arisah

Saptadi²,

Ashari³

et al. 2022

IOP Conf. Ser.: Earth Environ. Sci.

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“…Moreover, the alterations in irradiated flowers can be induced by variations in pigment quality and quantity caused by mutations in the anthocyanin biosynthetic pathway gene (Streisfeld et al 2013). A 40-100 Gy gamma irradiation dose, increased the levels of anthocyanins and flavonoids in Tulipa gesneriana (Li et al 2022), which may have led to darker flowers at a dose of 40-60 Gy. The change in the shape of the flower may be caused by changes in the placement of cells from meristem cells at the beginning of flower development as a result of mutations (Kumari and Kumar 2015;Li et al 2022).…”

Section: Flowermentioning

confidence: 99%

Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation

2022

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Abstract. Cahyaningsih AP, Etikawati N, Yunus A. 2022. Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation. Biodiversitas 23: 5351-5359. Indonesia was one of the countries that introduced E. purpurea as a medicinal plant. Accessions of E. purpurea that have been successfully cultivated in Indonesia have narrow genetic diversity, lack accession variation, and have almost uniform tillers. This study was conducted to determine the effect of different doses of gamma-ray irradiation on morphological characteristics of E. purpurea accession B2P2TOOT. The experimental design was a Randomized Block Design with three replications, and six doses of gamma-ray irradiation (0, 15 Gy, 20 Gy, 25 Gy, 40 Gy, and 60 Gy) were used. The qualitative morphological data were presented descriptively; quantitative data were analyzed using ANOVA followed by a DMRT test at a 5% level with SPSS 16.0 application. The Dice similarity algorithm analyzed the similarity index, group analysis, and dendrogram construction using the UPGMA method with the NTSYS 2.02 application. Gamma-ray irradiation treatment increased the survival rate of E. purpurea plants grown in tropical lowlands. A dose of 15-60 Gy gamma irradiation did not affect the qualitative morphology of E. purpurea roots, stems, and leaves. Irradiation at doses of 40 Gy and 60 Gy resulted in flowers with more variation in color, overall flower shape, and arrangement of ray floret. Gamma irradiation significantly affected plant height, leaf length, leaf area, flower angle, and the first day of flowering. The 40 and 60 Gy doses resulted in longer leaves with wider leaf surfaces. The dendrogram revealed that E. purpurea irradiation resulted in two main groups, with doses of 40 and 60 Gy forming their groups and increasing morphological variation by 30% compared to controls.

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“…Most probably, a forward mutation causes the phenomenon of stem color change. Li et al (2022) define forward mutation as a phenotypic change that always occurs continuously. Thus, it produces a new shape and color dissimilar from its original form.…”

Section: Qualitative Charactersmentioning

confidence: 99%

“…In particular, the induced mutation in ornamental plants emphasizes phenotypic changes and the addition of new color variations. Various studies on increasing the morphological diversity of commercial ornamental plants due to gamma-ray irradiation induction have been reported in Chrysanthemum (Din et al 2021), Hibiscus rosa-sinensis (Naveena et al 2020), Tulip (Li et al 2022), Lilium (Hajizadeh et al 2022), Celosia cristata (Muhallilin et al 2019), andOrchid (Hartati et al 2021). Increasing morphological variety through gammaray irradiation will increase the attractiveness and economic value of P. grandiflora.…”

Section: Introductionmentioning

confidence: 99%

The diversity of agromorphological characters of Portulaca grandiflora in the MV8 population deriving from recurrent irradiation

et al. 2022

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Abstract. Aisyah SI, Saraswati RAM, Yudha YS, Nurcholis W. 2022. The diversity of agromorphological characters of Portulaca grandiflorain the MV8 population deriving from recurrent irradiation. Biodiversitas 23: 4432-4439. A multipurpose ornamental plant, Portulaca grandiflora has the value of attractiveness and uniqueness, which lies in various agromorphological characters, primarily in flowers. The potential of P. grandiflora in qualitative characters such as new and unique shapes and colors of the morphology must be enhanced further to increase its economic and aesthetic significance as a floriculture commodity. The induced mutation technique has been proven to improve plants' morphological diversity, and even numerous novel cultivars are produced from artificial mutations. The recurrent irradiation technique was applied in the P. grandiflora mutation breeding program and produced new morphological variations of P. grandiflora until the MV7 generation (seventh generation of the mutation breeding program). The current study aimed to evaluate the diversity of morphological characters of P. grandiflora mutants in the MV8 population resulted from reccurent irradiation technique. Fifty-three selected mutants of P. grandiflora generation MV7 were multiplied by stem cuttings to create an MV8 population. The total MV8 population of P. grandiflora, the result of vegetative propagation, was 1104 plants. The diversity of morphological characters of P. grandiflora in the MV8 population was identified quantitatively (measurement) and qualitatively (visual observation). The results of this study reveal that several P. grandiflora mutants in the MV8 population have a new phenotypic appearance, chiefly in the qualitative characters of flowers. Besides, most of the P. grandiflora MV8 mutant population has inherited identical morphological characters from the previous generation (MV7 population). Further morphological analysis in the next generation needs to be conducted to evaluate the morphological stability of P. grandiflora mutants to develop new cultivars and to investigate the possibility of new phenotypic variations caused by recurrent irradiation treatments.

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Biological effects of gamma-ray radiation on tulip (Tulipa gesneriana L.)

Cited by 18 publications

References 58 publications

Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Disclosing the Genetic Diversity of ‘Earlibrite’ Strawberry Mutant Induced by Gamma-ray Irradiation Using ISSR Markers

Morphological characters variation of Indonesian accession Echinacea purpurea in response to gamma-ray irradiation

The diversity of agromorphological characters of Portulaca grandiflora in the MV8 population deriving from recurrent irradiation

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