Physiological Characterization and Emended Description of Methanolobus vulcani

Kadam, Priya C.; Boone, David R.

doi:10.1099/00207713-45-2-400

Cited by 29 publications

(8 citation statements)

References 15 publications

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“…Phylogenetically, A8p was most closely related to Methanobacterium formicicum (97.2 YO similarity). However, this evolutionary distance is sufficiently large that the two strains most probably belong in separate species (7,12,19,41). The phenotypic characteristics of A8p confirmed that the isolate belongs to the genus Methanobacterium and that it can be distinguished from phylogenetically related taxa as shown in Table 2.…”

Section: Discussionmentioning

confidence: 63%

Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater

Kotelnikova

Macario²,

Pedersen³

1998

International Journal of Systematic Bacteriology

132

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Deep subterranean granitic aquifers have not been explored regarding methanogens until now. Three autotrophic methane-producing Archaea were isolated from deep granitic groundwater a t depths of 68,409 and 420 m. These organisms were non-motile, small, thin rods, 01-0-15 pm in diameter, and they could use hydrogen and carbon dioxide or formate as substrates for growth and methanogenesis. One of the isolates, denoted A8p, was studied in detail. It grew with a doubling time of 2.5 h under optimal conditions (2040 "C, pH 7.8-8.8 and 0.2-1.2 M NaCI). Strain A8p is eurythermic as it grew between 3-6 and 45 "C. It was resistant to up to 20 mg bacitracin I-'. The G+C content was 54.5 mol0/o, as determined by thermal denaturation. Phylogenetic studies based upon 16s rRNA gene sequence comparisons placed the isolate A8p in the genus Methanobacterium Phenotypic and phylogenetic characters indicate that the alkaliphilic, halotolerant strain A8p represents a new species. We propose the name Methanobacterium subterraneum for this species, and strain A8p (= DSM 110749 is the type strain.

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Section: Discussionmentioning

confidence: 63%

Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater

Kotelnikova

Macario²,

Pedersen³

1998

International Journal of Systematic Bacteriology

132

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“…M. barkeri is a metabolically versatile methanogen and can grow on hydrogen, methylated compounds, methanol, or acetate (70,71), while M. vulcani uses only methanol as a growth substrate (72). However, in the presence of elemental sulfur, as detected at the biofilm-metal interface in the corroding piping section, these methanogens, as well as Methanobacterium species, can switch their metabolism to dissimilatory elemental sulfur reduction and produce high concentrations of hydrogen sulfide (73), potentially contributing to CMIC in this way (Fig.…”

Section: Discussionmentioning

confidence: 99%

Complementary Microorganisms in Highly Corrosive Biofilms from an Offshore Oil Production Facility

Vigneron

Alsop

Chambers

et al. 2016

Appl Environ Microbiol

138

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e Offshore oil production facilities are frequently victims of internal piping corrosion, potentially leading to human and environmental risks and significant economic losses. Microbially influenced corrosion (MIC) is believed to be an important factor in this major problem for the petroleum industry. However, knowledge of the microbial communities and metabolic processes leading to corrosion is still limited. Therefore, the microbial communities from three anaerobic biofilms recovered from the inside of a steel pipe exhibiting high corrosion rates, iron oxide deposits, and substantial amounts of sulfur, which are characteristic of MIC, were analyzed in detail. Bacterial and archaeal community structures were investigated by automated ribosomal intergenic spacer analysis, multigenic (16S rRNA and functional genes) high-throughput Illumina MiSeq sequencing, and quantitative PCR analysis. The microbial community analysis indicated that bacteria, particularly Desulfovibrio species, dominated the biofilm microbial communities. However, other bacteria, such as Pelobacter, Pseudomonas, and Geotoga, as well as various methanogenic archaea, previously detected in oil facilities were also detected. The microbial taxa and functional genes identified suggested that the biofilm communities harbored the potential for a number of different but complementary metabolic processes and that MIC in oil facilities likely involves a range of microbial metabolisms such as sulfate, iron, and elemental sulfur reduction. Furthermore, extreme corrosion leading to leakage and exposure of the biofilms to the external environment modify the microbial community structure by promoting the growth of aerobic hydrocarbon-degrading organisms.

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“…Sequences of OTU 11 could be classified to the genus Methanolobus and were closely related to Methanolobus vulcani (98%), which is halotolerant and uses methyl-group containing substrates for growth (Kadam and Boone 1995). Sequences of OTU 12 were assigned to the genus Methanoculleus with a high similarity to Methanoculleus marisnigri (97%) (Romesser et al 1979) and Methanoculleus palmolei (98%) (Zellner et al 1998 rDNA of Methanobacterium curvum (96%, OTU 13) (Sun et al 2001), a hydrogenotrophic methanogen.…”

Section: Analyses Of Clone Librariesmentioning

confidence: 99%

Predominance ofMethanolobusspp. andMethanoculleusspp. in the Archaeal Communities of Saline Gas Field Formation Fluids

Ehinger

Seifert

Kassahun

et al. 2009

Geomicrobiology Journal

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The microbial communities in sulfate-rich, saline formation fluids of a natural gas reservoir in Lower Saxony, Germany were investigated to enhance the knowledge about microbial communities in potential carbon dioxide sequestration sites. This investigation of the initial state of the deep subsurface microbiota is necessary to predict their influence on the long-term stability and storage capacity of such sites. While the bacterial 16S rDNA gene library was comprised of sequences affiliating with the Firmicutes, the Alphaproteobacteria, the Gammaproteobacteria and the Thermotogales, the archaeal 16S rDNA libraries were simply dominated by two phylotypes related to the genera Methanolobus and Methanoculleus. The monitoring of the archaeal communities in different formation fluid samples by T-RFLP and Real-Time-PCR indicated that these two methanogenic genera dominated at all, whereas the proportion of the two groups varied. Thus, methylotrophic and autotrophic methanogenesis seems to be of importance in the reservoir fluids, dependent on the provided reduction equivalents and substrates and it also may influence the fate of CO 2 in the subsurface.

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Physiological Characterization and Emended Description of Methanolobus vulcani

Cited by 29 publications

References 15 publications

Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater

Methanobacterium subterraneum sp. nov., a new alkaliphilic, eurythermic and halotolerant methanogen isolated from deep granitic groundwater

Complementary Microorganisms in Highly Corrosive Biofilms from an Offshore Oil Production Facility

Predominance ofMethanolobusspp. andMethanoculleusspp. in the Archaeal Communities of Saline Gas Field Formation Fluids

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