Physiological characteristics of epididymal spermatozoa from postmortem rams

Garde, José Julián; Mj, Aguado; Pérez, Susan; Garrido, D.; Pérez-Guzmán, M. D.; Montoro, Vidal

doi:10.1016/s0093-691x(05)80113-x

Cited by 24 publications

(10 citation statements)

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“…The pressure pushes the sperm toward an incision in the cauda epididymis, generally near the junction with the corpus, where the gametes are recovered. 82 It allows samples to be obtained with a lower level of contamination by blood cells. 83 Sperm cells derived from this technique have been efficiently cryopreserved in red deer, 84 African buffalos, 85 and agoutis.…”

Section: The Preservation Of Male Gametesmentioning

confidence: 99%

Cryopreservation in mammalian conservation biology: current applications and potential utility

Silva¹,

Lima²,

Peixoto³

et al. 2015

RRBS

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A rapid and continuous decline in wild mammalian species populations has been documented in recent decades. Although in situ conservation strategies such as habitat preservation are usually the best way to preserve biodiversity, other rescue strategies such as germplasm cryopreservation are sometimes necessary. Germplasm cryopreservation involves the freezing of gametes, embryos, gonadal tissues, or somatic tissues of species threatened with extinction. There is substantial diversity in the cryobiological requirements among cell types and tissues of each species. Research has focused on adapting techniques developed for the conservation of the genetic material of domestic animals for use with wild species about which relatively little is known. This review describes and discusses the current and potential use of cryobanking for the preservation of cells and tissues of threatened species.

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Section: The Preservation Of Male Gametesmentioning

confidence: 99%

Cryopreservation in mammalian conservation biology: current applications and potential utility

Silva¹,

Lima²,

Peixoto³

et al. 2015

RRBS

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“…Several methods of sperm recovery from cauda epididymis have been described, including aspiration (Sharma et al, 1997), flotation -in which sliced epididymis is placed on gel medium for some time (Hewitt et al, 2001), and retrograde flush of the cauda -in which pressure is generated by a syringe attached to the vas deferens and sperm is carried by the extender and expelled through a cut on the junction of cauda and corpus (Garde et al 1994). -Pastor et al (2006) compared the retrograde flush and flotation techniques for the recovery of epididymal sperm and they have obtained a higher number of sperm using retrograde flush.…”

Section: Collection Of Epididymal Spermmentioning

confidence: 99%

Advances in stallion’s epididymal sperm technology

Melo¹,

Monteiro²,

Avanzi³

et al. 2010

PHK

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SummaryFreezing epididymal sperm is a method to preserve germplasm from animals with not only high genetic potential but also endangered species. In the equine some owners have chosen this possibility in cases of either severe illness or death of stallions. However, the lack of knowledge and poor published results of such technique hampers its propagation. New procedures have allowed some improvement on fertility rates of frozen sperm from the epididymis of stallions. The aim of this study is to report the advances on processing and cryopreservation of samples from the stallion's epididymal semen.

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“…A técnica de fluxo retrógrado relatada por Garde et al, (1994) (GRANEMANN, 2006). Esta função de ativação é exercida pelo plasma seminal em condições fisiológicas durante a ejaculação, como relatado por Tiplady et al (2002).…”

Section: Discussionunclassified

“…A técnica de fluxo retrógrado na cauda do epidídimo consiste na geração de pressão com o uso de uma seringa nos vasos deferentes até que os espermatozóides epididimários sejam carreados pelo diluente e extravasem pelo corte realizado na junção entre a cauda e o corpo do epidídimo (GARDE et al, 1994). Com esta técnica, o número de espermatozóides recuperados da cauda do epidídimo foi superior àquele obtido do ejaculado (GRANEMANN, 2006).…”

Section: Fluxo Retrógrado Da Cauda Do Epidídimounclassified

“…A colheita dos espermatozóides da cauda do epidídimo foi realizada pela da técnica de fluxo retrógrado relatada por Garde et al (1994) A avaliação da concentração espermática (mm 3 ) foi determinada por meio de câmara hematocitométrica, sob microscopia com contraste de fase 7 em aumento de 100 a 400 vezes. Para isso, utilizou-se uma alíquota da amostra diluída na proporção de 10 µl da amostra para 990 µl de água destilada (1:100).…”

Section: Colheita Dos Espermatozóides Da Cauda Do Epidídimounclassified

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Criopreservação e fertilidade de espermatozóides recuperados da cauda do epidídimo de garanhões

Monteiro¹

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Physiological characteristics of epididymal spermatozoa from postmortem rams

Cited by 24 publications

References 0 publications

Cryopreservation in mammalian conservation biology: current applications and potential utility

Cryopreservation in mammalian conservation biology: current applications and potential utility

Advances in stallion’s epididymal sperm technology

Criopreservação e fertilidade de espermatozóides recuperados da cauda do epidídimo de garanhões

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