Physiological characteristics and cold tolerance of overwintering eggs in Gomphocerus sibiricus L. (Orthoptera: Acrididae)

Abstract

Gomphocerus sibiricus L., the dominant insect species in the alpine and subalpine grassland, overwinters with diapause at egg stage. In this study, cold tolerance and related cryoprotectants of G. sibiricus eggs were investigated. In particular, the supercooling point (SCP), water content, carbohydrates (trehalose, glucose, fructose, glycogen), polyols (glycerol, inositol, sorbitol), fat, and amino acids contents were evaluated at different developmental stages of G. sibiricus eggs collected under natural conditions. The SCPs of eggs were very low (−32.83 to −22.61°C) at middiapause. Water content gradually increased during development. The fructose, glycerol, and sorbitol contents were significantly higher in diapausing eggs than in early embryogenesis stage and post-diapause development stage.Glycogen content was high throughout the whole developmental period. The trehalose, glucose, and inositol contents were low during diapause compared to that in early embryogenesis stage and post-diapause development stage. There were no significant differences in the fat content of eggs among all development stages. The total amino acid contents in eggs in the early embryogenesis and at the start of diapause were higher than that in

Natural multi-osmolyte cocktails form deep eutectic systems of unprecedented complexity: discovery, affordances and perspectives

“…The way cells regulate the stabilization of macromolecules at low water content conditions (freezing and drought) could also be explained by the reversibility of DESs formation and dilution. In psychrophilic bactera, 92 diapausing insects 93,94 ( e.g. grasshoppers and spiders), amphibians 51 ( e.g.…”

Predictions based on different climate change scenarios: The habitat of typical locust species is shrinking in Xinjiang, China, and Kazakhstan

“…The Italian locust, Asian Migratory locust, and Siberian locust are common pests in steppe, desert, and semidesert regions. Due to their longdistance migrations, the locusts can at times be found in the territories ranging from the Ukraine to southern Siberia, eastern Kazakhstan, and northwestern China, causing severe damage to local steppe ecosystems and agricultural production (Jamal et al, 2021;Song et al, 2021). The occurrence of locust infestations is continuous and results from a combination of environmental factors and ecological conditions.…”

Predictions Based on Different Climate Change Scenarios: The Habitat of Typical Locust Species Is Shrinking in Kazakhstan and Xinjiang, China

“…The species share some similarities in bioecology and population dynamics, and therefore some overlap in habitat selection [ 3 , 4 ]. The Italian locust is often found from Ukraine to southern Siberia, eastern Kazakhstan, and northwestern China, while the Asian locust and Siberian locust have a much wider range, covering the entire temperate and steppe regions of Eurasia, causing severe damage to local steppe ecosystems and agricultural production [ 5 , 6 , 7 ]. The occurrence of locust infestations is continuous and results from a combination of environmental factors and ecological conditions.…”