Physiological Characteristics and Anti-Obesity Effect of Lactobacillus plantarum K6 isolated from Kimchi

Abstract

This study aimed to investigate the physiological characteristics and anti-obesity effects of a newly isolated bacterium, Lactobacillus plantarum K6. L. plantarum K6 showed good α-amylase inhibitory activity (96.78±3.29%), α-glucosidase inhibitory activity (92.55±9.62%), and lipase inhibitory activity (85.17±0.79%), and the strain inhibited the adipocyte differentiation of 3T3-L1 cells (27.4±1.4%) when present at a concentration of 100 μg/mL. L. plantarum K6 was isolated from kimchi and its physiological characteristics were investigated. A comparison of the sensitivity of the isolate to 15 different antibiotics showed that L. plantarum K6 is highly sensitive to erythromycin and highly resistant to vancomycin, ampicillin, and polymyxin B. This strain also showed high arylamidase and β-galactosidase activities. Moreover, it was relatively tolerant to bile acid and low pH, and displayed resistance to Escherichia coli, Salmonella Typhimurium, Listeria monocytogenes, and Staphylococcus aureus, with rates of 51.8%, 42.4%, 61.6%, and 54.9%, respectively. No bio genic amines were produced. L. plantarum K6 also showed high adhesion activity to HT-29 cells compared to L. rhamnosus GG. These results demonstrate that Lactobacillus plantarum K6 has potential as a probiotic with anti-obesity effects. Keywords

In Vitro Antidiabetic, Antioxidant Activity, and Probiotic Activities of Lactiplantibacillus plantarum and Lacticaseibacillus paracasei Strains

“…In particular, MG4296 showed the highest inhibition on α-glycosidase (Table 1). In a previous study, Kim et al [34] demonstrated that Lactiplantibacillus (Lpb.) plantarum K10 effectively inhibited α-amylase activity by 94.6%.…”

Lactobacillus fermentum SMFM2017-NK4 Isolated from Kimchi Can Prevent Obesity by Inhibiting Fat Accumulation

“…EI-shiekh et al (2019) [ 27 ] showed that the activities of various plant extracts including Solenostemma argel were 37.29%–89.08%. Kim and Lim (2020) [ 28 ] reported that α-glucosidase inhibitory activity of the milk fermented with L. plantarum KI134 strain was 2.74%, which was the highest among other 255 strains. Compared to these activities, the α-glucosidase inhibitory activity in our study presented above can be considered similar or high.…”

“…Kim et al (2010) [ 34 ] evaluated the effect of soy milk fermented with Bacillus subtilis KC-3 on the inhibition of 3T3-L1 cell differentiation and found that the fermented soy milk reduced 19% of fat accumulation, and Samjeonghwan fermented with lactic acid bacteria reduced the number of adipocytes [ 35 ]. In a study by Kim and Lim (2017) [ 36 ], L. plantarum K6 was selected as a lactic acid bacterium that has an anti-obesity effect; it inhibited differentiation of 3T3-L1 by 27.4%. These are similar to the highest inhibitory value of 21.44% (strain LAB 73) obtained in our study ( Table 5 ).…”

Therapeutic and Improving Function of Lactobacilli in the Prevention and Treatment of Cardiovascular-Related Diseases: A Novel Perspective From Gut Microbiota

“…L. plantarum has important effects in preventing hypercholesterolemia, hyperlipidemia, atherosclerosis, T2DM, obesity, and hypertension. L. plantarum can absorb cholesterol directly from the culture medium and was thus selected as a probiotic that potentially reduced cholesterol levels in mice/rats (86,(146)(147)(148)(149)(150)(151)(152)(153)(154). L. plantarum HT121 improved serum lipid profiles, restored beneficial gut microbes, and regulated BAs metabolism (155).…”