Physiological aspects of l-lysine production: effect of nutritional limitations on a producing strain of Corynebacterium glutamicum

Abstract

The effect of nutritional limitations, such as phosphorus and carbon, on the production of L-lysine by Corynebacterium glutarnicum was studied in continuous culture. We observed that phosphate-limited cultures at low growth rates were favourable to L-lysine production. L-Lysine was produced when a culture at low dilution rate (0.03 h-~) was established. A dilution rate of about 0.04 h -1 should be maintained in order to assure good productivity and an L-lysine yield of 0.53 g/ g. Under carbon-limiting conditions the maintenance energy and growth yield of 0.03 g / g . g -1. h -1 and 0.41 g/g, respectively, have been obtained. Under these limiting conditions the L-lysine production was not favoured even at lower dilution rates.

Gluconate as suitable potential reduction supplier in Corynebacterium glutamicum: Cloning and expression of gntP and gntK in Escherichia coli

“…These characteristics, and the enhancement of Gnd specific activity in gluconate containing cultures, which is mainly addressed to generate reducing power, might facilitate the search for strategies to improve the efficiencies of C. glutamicum in production conditions. It is known that the yield of Llysine by this bacterium is increased if cultivated in glucose plus gluconate (Lee et al, 1998;Coello et al, 1992). This result, as well as ours, can be explained by the coordinate and negative regulation of GntR1 and GntR2, two redundant repressors of gntP, gntK and gnd whose actions are interfered by gluconate (Frunzke et al, 2008).…”

Synthesis and thermal behavior of thermotropic terpolymers based on 8‐(3‐hydroxyphenyl)octanoic acid, 2,6‐naphthalenedicarboxylic acid, and substituted hydroquinones

“…Likewise, citric acid production was favored at low phosphate concentrations in Aspergillus niger fermentation 19. A similar effect was observed in chemostat cultures of Corynebacterium glutamicum where phosphate limiting conditions at low dilution rates induced higher lysine 20. Therefore, it was of interest to study the effect of phosphate levels on D‐ribose production.…”

Reaction engineering analysis of L-lysine transport by Corynebacterium glutamicum

“…In addition to the previously described modulation of carrier activity by membrane potential, pH, and L-lysine gradients across the cell the regulation 8. 5 investigated. This regulation was assumed to most likely take place on the level of cell-specific export carrier concentration (C).…”