Physiological and ultrastructural effects of lead on tobacco

Alkhatib, Rami; Maruthavanan, Janakiraman; Ghoshroy, Soumitra; Steiner, R.; Sterling, Tracy M.; Creamer, Rebecca

doi:10.1007/s10535-012-0241-9

Cited by 24 publications

(15 citation statements)

References 23 publications

(31 reference statements)

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“…The growth of legume plants grown on Pb ore tailings was reported to be drastically affected [28]. Pb also inhibited root and shoot growth in tobacco [48], and wheat [7]. Similarly, in the present study, the root and shoot growth of cultivar K6 and cultivar K9 were inhibited by Pb-stress and the reduction was found to be concentration dependent.…”

Section: Discussionsupporting

confidence: 77%

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Nareshkumar¹,

Veeranagamallaiah²,

Pandurangaiah³

et al. 2015

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Lead (Pb) is an important environmental pollutant extremely toxic to plants and other living organisms including humans. To assess Pb phytotoxicity, a pot culture experiment was carried out using two groundnut cultivars (Arachis hypogaea L. cultivar K6 and cultivar K9) on plant growth, ROS levels, lipid peroxidation, and antioxidant metabolism using biochemical, histochemical methods. Plants were grown in pots for 14 days, in the botanic garden, and subjected to Pb-stress (0, 100, 200, 400 and 800 ppm) by adding Pb (NO3)2 solution and further allowed to grow for 10 days. The results showed that cultivar K6 registered lower Pb accumulation than cultivar K9, however, localization of Pb was greater in roots than leaves in both groundnut cultivars. The Pb-stress results in an increase in free radicals (O2• − and H2O2) generation in both groundnut cultivars, but more significantly in cultivar K9 than K6. Pb-stress also caused significant changes in the rate of peroxidation as shown in the levels of malondialdehyde (MDA) content in roots and leaves of both groundnut cultivars. Free proline, ascorbic acid (AsA) and non-protein thiol (NP-SH) contents were increased in cultivar K6 due to Pb-stress, but less in cultivar K9. Pb treated plants showed increased levels of antioxidant enzymes such as superoxide dismutase (SOD), guaiacol peroxidase (GPX), ascorbate peroxidase (APX) glutathione reductase (GR) and glutathione Stransferase (GST). Isozyme band intensities of SOD, GPX and APX were more consistent with the respective changes in antioxidative enzyme activities. These results indicate that cultivar K6 possesses greater tolerance potential for Pb toxicity than cultivar K9.

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Section: Discussionsupporting

confidence: 77%

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Nareshkumar¹,

Veeranagamallaiah²,

Pandurangaiah³

et al. 2015

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“…The growth of legume plants grown on Pb ore tailings was reported to be drastically affected [19]. Pb also inhibited root and shoot growth in tobacco [20] and wheat [21]. Similarly, in the present study, the root and shoot growth of cultivar K6 and cultivar K9 were inhibited by Pb stress and the reduction was found to be concentration dependent (table 1).…”

Section: Effect Of Pb Stress On Plant Growthsupporting

confidence: 78%

Effect of Pb-Stress on Growth and Mineral Status of Two Groundnut (Arachis hypogaea L.) Cultivars

Nareshkumar¹,

Krishnappa²,

Kirankumar³

et al. 2014

JPS

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Abstract:Heavy metal pollution of air and agricultural soils is one of the most important ecological problems on world scale.Among the heavy metals, lead (Pb) is one of the common environmental pollutants. To investigate Pb effects on nutrient uptake, two groundnut (Arachis hypogaea L.) cultivars (cultivar K6 and cultivar K9) were grown in pot cultures and stressed with lead nitrate (Pb(NO 3 ) 2 ) at four concentrations (100, 200, 400 and 800 ppm). Pb is accumulated in roots and leaf tissues in dose dependent manner in both groundnut cultivars, which resulted in reduced root and shoot growth and lower uptake of all mineral ions tested. The content of mineral ions such as Ca, Na, Mg, Co, Cu, Ni, Zn and Mn reduced in root and leaf tissues of both cultivars due to Pb stress. But the reduction in mineral ion content was less in cultivar K6 than in cultivar K9. The deficiency of mineral nutrients correlates in a strong decrease in the contents of total chlorophyll, and anthocyanin in both cultivars, but these effects are less pronounced in cultivar K6 than in cultivar K9.

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“…Lead-treated tobacco genotypes showed significant reduction in photosynthesis and other related parameters. Lead is known to induce stomatal closure, thereby inhibiting gaseous exchange, which impacts negatively on photosynthetic rates [35]. The latter scenario was also confirmed by Shu et al [36].…”

Section: Discussionmentioning

confidence: 75%

Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

Maodzeka

Hussain

Wei

et al. 2016

Enviro Toxic and Chemistry

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In the present study, the effects of lead (Pb) uptake and toxicity were investigated in a hydroponic culture using 7 tobacco (Nicotiana tabacum L.) genotypes (Bina 1 [B1], Kutsaga Mammoth 10 [KM10], Nanjing 3 [N3], Kutsaga 35 [K35], Kutsaga E1 [KE1], Cocker 176 [C176], and Kutsaga RK6 [KRK6]) that differed in Pb tolerance. Lead was applied as a solution of Pb nitrate at concentrations of 0 μM, 10 μM, 250 μM, and 500 μM. After 4 wk of Pb treatment, tissue biomass and photosynthetic parameters were measured and elemental analysis was performed. The results showed decreases in growth and photosynthetic parameters with increases in Pb concentration compared with the control. The least reduction in the recorded physiological parameters was noted in K35, whereas the greatest reduction was observed in N3, which is an obvious indication of genotypic differences. Activities of peroxidase, catalase, and malondialdehyde increased significantly with increases in Pb concentration, with genotypes K35 and N3 showing the least and the greatest reduction, respectively. The results demonstrate the phototoxic nature of Pb on plants, and it can be concluded that in Pb-prone areas genotypes K35 and B1 can be used for cultivation because they can grow efficiently in the presence of high Pb concentrations while restricting Pb uptake in the aboveground parts, as seen by the higher Pb tolerance index. Environ Toxicol Chem 2017;36:175-181. © 2016 SETAC.

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Physiological and ultrastructural effects of lead on tobacco

Cited by 24 publications

References 23 publications

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Effect of Pb-Stress on Growth and Mineral Status of Two Groundnut (Arachis hypogaea L.) Cultivars

Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

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Physiological and ultrastructural effects of lead on tobacco

Cited by 24 publications

References 23 publications

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.) Cultivars

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (&lt;i&gt;Arachis hypogaea&lt;/i&gt; L.) Cultivars

Effect of Pb-Stress on Growth and Mineral Status of Two Groundnut (Arachis hypogaea L.) Cultivars

Elucidating the physiological and biochemical responses of different tobacco (Nicotiana tabacum) genotypes to lead toxicity

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Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars

Pb-Stress Induced Oxidative Stress Caused Alterations in Antioxidant Efficacy in Two Groundnut (<i>Arachis hypogaea</i> L.) Cultivars