Physiological and ultra-structural changes in Brassica napus seedlings induced by cadmium stress

Ali, Basharat; Qian, Ping; Jin, Rong; Ali, Shafaqat; Khan, Muhammad İmran; Aziz, Rukhsanda; Tian, Tongyan; Zhou, Weijun

doi:10.1007/s10535-013-0358-5

Cited by 147 publications

(60 citation statements)

References 60 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Spatial distribution has been demonstrated for accumulation of reduced GSH, PCs (Dra̧zkiewicz et al 2003) as well as for activity of H + ATPase (Kaznina et al 2013). Such variance in responsiveness to metals is given (at least partially) by anatomic, morphologic, physiological or biochemical changes characteristic for each developmental stage Vaculík et al 2012;Ali et al 2014), but also by specific allocation of defense components through the plants (Dra̧zkiewicz et al 2003;Dra̧zkiewicz and Baszyński 2005;Fluch et al 2008). Nonetheless, within-plant distribution of inducible defense compounds has been observed for other stress types, too (Visker et al 2003;Jerkovic et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Variable responses of soybean chitinases to arsenic and cadmium stress at the whole plant level

et al. 2014

View full text Add to dashboard Cite

Plant chitinases (EC 3.2.1.14) are considered as typical defense components under various environmental stresses, including heavy metals. In addition, some of them play crucial role in normal plant growth and development. In this work the profile and activities of these enzymes were analyzed to study the variability of defense within soybean plants. For this, two cultivars with contrasting tolerance to metals were exposed to ecologically relevant doses of arsenic and cadmium. Enzyme profiles revealed a spatial distribution of chitinase activities throughout the individual plants, tending to decrease upwards to the top of the plants. Under metal stress, there was a single responsive isoform detected in roots that behaved opposingly in the studied soybean cultivars. In contrast, several isoforms were activated in aboveground tissue, predominantly in mature (older) leaves. Of these, two were identified (21 and 42 kDa) as more specifically involved in defense against metal stress in soybean. The 21 kDa isoform was concluded as possibly contributing to metal tolerance and deserves further investigations at molecular level. Nevertheless, no sound interaction was detected between leaf developmental stage and responsiveness to metals for either of the chitinase isoforms. Further studying the distribution of induced defense within plants is important in understanding the defense strategy of plants against environmental cues including metals.

show abstract

Section: Introductionmentioning

confidence: 99%

Variable responses of soybean chitinases to arsenic and cadmium stress at the whole plant level

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Gill et al (2014) also reported genotypic variation in response to Cr toxicity in four oilseed rape cultivars. According to a study by Ali et al (2014), the stress responses of two cultivars of B. napus exposed to same concentrations of Cd showed different responses. Feigl et al (2013) reported that when exposed to Cu excess the morphological adaptation strategy, especially, the root system development in B. juncea and B. napus showed differences.…”

Section: Introductionmentioning

confidence: 99%

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Nair

Chung

2017

3 Biotech

View full text Add to dashboard Cite

Rapid growth of nanotechnology has enabled the production and use of engineered nanoparticles (ENPs) in several industries as well as in agriculture areas. This has raised ecotoxicological concerns due to the release of ENPs to the environment. In the present study, we investigated the effects of interactions of copper oxide nanoparticles (CuO NPs) on physiological, biochemical, and molecular indices in seedlings of an important oil seed crop Brassica napus L. The seedlings were treated with 0, 20, 50, 100, 200, 400, and 500 mg/L of CuO NPs for 14 days in halfstrength semi-solid Murashige and Skoog medium. The CuO NPs treatment significantly reduced shoot and root growth as well as plant biomass. Shortening and thickening of primary and lateral roots and inhibition of lateral root growth was observed at higher concentrations. An increase in reactive oxygen species generation, and malondialdehyde accumulation was observed. Histochemical staining of roots with propidium iodide and aniline blue indicated cell death and callose formation in roots. Transcriptional modulation of genes related to oxidative stress viz. CuZn superoxide dismutase, catalase, and ascorbate peroxidase was observed. Element content analysis showed an increase in Cu content and decrease in Fe, Mn, and Zn contents. Overall, exposure to CuO NPs caused oxidative injury, cell death, callose formation, and decreased the micro nutrient contents in B. napus seedlings.

show abstract

“…The interest in this species is derived from its properties of rapid growth, high biomass production, and relative resistance to metal stress (Yu et al 2012), which made it a candidate plant for phytoremediation, along with species like B. juncea, Helianthus annuus, and Zea mays (Yadav and Srivastava 2014). Despite such importance, however, little is known about the toxicity response of Brassica napus plants against metal mixture stress and the interactive toxic effects of Cd and Cu, although extensive literature exist on individual response of Cd and Cu stress on plants (Burkhead et al 2009;Ali et al 2014;Andresen and Küpper 2013;Ravet and Pilon 2013). A few studies have previously investigated the combined toxicity of Cd and Cu in terrestrial plants including B. chinensis (Wong et al 1986), Picea sitchensis (Burton et al 1986), carrot (Al-Subu et al 1993), cucumber , and more recently barley (Ž altauskait_ e and Š liumpait_ e 2013), but pretty varied trend of results have been obtained.…”

Section: Introductionmentioning

confidence: 99%

Interactive effects of cadmium and copper on metal accumulation, oxidative stress, and mineral composition in Brassica napus

Mwamba

Ali

et al. 2016

Int. J. Environ. Sci. Technol.

Self Cite

View full text Add to dashboard Cite

Heavy metals' frequent occurrence and toxicity caused considerable concerns in assessing the interactive effects of metals on exposed plants. Therefore, a hydroponic study was conducted to assess the growth response and physio-chemical changes in Brassica napus plants under single and combined stress of two environmentally alarming metals (Cd and Cu). Results showed that 15-day metal exposure to different metal concentrations (0, 50, 200 lM) significantly enhanced Cd accumulation, while lesser extent of Cu was observed in plant tissues. Nonetheless, Cu caused more pronounced oxidative damages and plant growth retardation. Both metals showed similar trend of changes in mineral composition, although Cu proved more damaging effect on K and Mn contents, and Cd on Zn contents. In combined treatments, Cd stimulated Cu uptake, notably at low concentration, while its own uptake was restricted by the presence of Cu. At either level of concentration, combined stress of these metals exacerbated plant growth inhibition and caused further oxidative damages compared to their individual stress. However, metals synergistic effects occurred only in conditions where Cu uptake was enhanced by Cd. A greater synergistic effect was observed in sensitive cultivar Zheda 622 as compared to the tolerant cultivar ZS 758. As to mineral composition, no metals synergistic effects were noted. This study highlighted the ecotoxicological significance of Cd-led Cu uptake in B. napus, which was assumed to drive metals' synergistic toxicity, and showed that the relationship between Cd-led Cu uptake and plant growth responses could vary with respect to cultivar.

show abstract

Physiological and ultra-structural changes in Brassica napus seedlings induced by cadmium stress

Cited by 147 publications

References 60 publications

Variable responses of soybean chitinases to arsenic and cadmium stress at the whole plant level

Variable responses of soybean chitinases to arsenic and cadmium stress at the whole plant level

Evaluation of stress effects of copper oxide nanoparticles in Brassica napus L. seedlings

Interactive effects of cadmium and copper on metal accumulation, oxidative stress, and mineral composition in Brassica napus

Contact Info

Product

Resources

About