Physiological and transcriptome analyses highlight multiple pathways involved in drought stress in Medicago falcata

Li, Qian; Gu, Lili; Song, Jiaxing; Li, Chenjian; Zhang, Yanhui; Wang, Yuxiang; Pang, Yongzhen; Zhang, Bo

doi:10.1371/journal.pone.0266542

Cited by 6 publications

(4 citation statements)

References 57 publications

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“…The abundances of pyruvate, phosphate dikinase, and phosphoenolpyruvate carboxykinase increased, consuming ATP to convert pyruvate into phosphoenolpyruvate and participating in the process of gluconeogenesis. The glycolysis/gluconeogenesis pathway in plants has been repeatedly confirmed to be involved in the drought response [ 21 , 22 ], which may play a major role in accumulating sugars and protecting cells. We determined the soluble sugar content in the leaves of the drought and MC treatment groups, as shown in Figure 7 , and found that the soluble sugar content in the leaves increased by 8.41% after MC pretreatment.…”

Section: Resultsmentioning

confidence: 99%

Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics

Dong,

Wang,

et al. 2023

Plants

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Soybeans are the main sources of oil and protein for most of the global population. As the population grows, so does the demand for soybeans. However, drought is a major factor that limits soybean production. Regulating soybean response to drought stress using mepiquat chloride (MC) is a feasible method; however, its mechanism is still unclear. This study used PEG-6000 to simulate drought stress and quantitative proteomic techniques to reveal changes in Heinong44 (HN44) and Heinong65 (HN65) subjected to drought following the application of 100 mg/L of MC. The results showed that SOD in HN44 did not change significantly but decreased by 22.61% in HN65 after MC pretreatment, and MDA content decreased by 22.75% and 21.54% in HN44 and HN65, respectively. Furthermore, MC improved the GSH–ASA cycle and simultaneously promoted the Calvin cycle process to enable the plant to maintain a certain carbon assimilation rate under osmotic stress. In addition, MC upregulated some proteins during gluconeogenesis and starch metabolism and increased soluble sugar content by 8.41% in HN44. MC also reduced ribosomal protein abundance, affecting translation and amino acid metabolism. In summary, MC improved GSH–ASA cycle and Calvin cycle under stress to alleviate oxidative damage and maintain crop growth. Our study is the first to report the mechanism of MC regulation in soybean under osmotic stress, providing new insights for the rational application of MC in soybean.

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Section: Resultsmentioning

confidence: 99%

Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics

Dong,

Wang,

et al. 2023

Plants

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“…Carotenoid biosynthesis pathways have been reported to be enhanced in plants under environmental stress, which, in turn, increases carotenoid content [22]. Fatty acids were beneficial to plant resistance to environmental stress, and the higher their content, the stronger plant resistance to environmental stress [23]. The increased capacity of pentose and glucuronate interconversions was beneficial in improving carbohydrate and energy metabolism, which, in turn, promoted plant metabolism and enhanced plant resistance to environmental stress [24].…”

Section: Kegg Pathway Enrichment and Functional Analysis Of Key Genesmentioning

confidence: 99%

Transcriptomic Analysis of the Effect of Pruning on Growth, Quality, and Yield of Wuyi Rock Tea

Zhang,

Wang

et al. 2023

Plants

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Pruning is an important agronomic measure in tea plantation management. In this study, we analyzed the effect of pruning on gene expression in tea leaves from a transcriptomics perspective and verified the results of a transcriptomic analysis in terms of changes in physiological indicators of tea leaves. The results showed that pruning enhanced the gene expression of nine metabolic pathways in tea leaves, including fatty acid synthesis and carbohydrate metabolism, nitrogen metabolism, protein processing in the endoplasmic reticulum, and plant hormone signal transduction, thereby promoting the growth of tea plants and increasing tea yield. However, pruning reduced the gene expression of nine metabolic pathways, including secondary metabolites biosynthesis, flavonoid biosynthesis, phenylpropanoid biosynthesis, and sesquiterpenoid and triterpenoid biosynthesis, and lowered the content of caffeine, flavonoids, and free amino acids in tea plant leaves. In conclusion, pruning could promote the growth of tea plants and increase the yield of tea, but it was not conducive to the accumulation of some quality indicators in tea leaves, especially caffeine, flavonoids, and free amino acids, which, in turn, reduced the quality of tea. This study provides an important theoretical reference for the management of agronomic measures in tea plantations.

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“…Plants respond to drought by increasing antioxidant enzyme activity, which removes reactive oxygen species to alleviate stress caused by drought. In the antioxidant enzyme system, superoxide radicals can be made disproportionate to H 2 O 2 and O 2 by SOD (superoxide dismutase), while H 2 O 2 is broken down by POD (peroxidase) and CAT (catalase) [12]. In addition to enzymatic antioxidants (e.g., antioxidant enzymes such as SOD and POD), non-enzymatic antioxidants (e.g., flavonoids) are also involved in antioxidant defense in plants.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome Analysis on the Quality of Epimedium koreanum in Different Soil Moisture Conditions at Harvesting Stage

Zhang,

Wang,

et al. 2024

Genes

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Epimedium koreanum is a traditional Chinese tonic herb. Its main medicinal components are secondary metabolites such as flavonoids and flavonol glycosides, but the biosynthetic mechanism is still unclear. Moisture conditions are a key environmental factor affecting E. koreanum medicinal components during harvesting. Different stages of E. koreanum under natural conditions after rainfall were selected to study changes in physiological properties, herb quality, and transcriptome. Malondialdehyde (MDA) content increased significantly in the D3 stage after rainfall, and protective enzyme levels also rose. Additionally, the flavonol glycoside content was relatively high. We sequenced the transcriptomes of D1, D3, and D9 (R) and identified differentially expressed genes (DEGs) related to flavonoid synthesis. This analysis allowed us to predict the roadmap and key genes involved in flavonoid biosynthesis for E. koreanum. These results suggest that the E. koreanum quality can be enhanced by natural drought conditions in the soil after precipitation during harvest. The harvesting period of E. koreanum is optimal when soil moisture naturally dries to a relative water content of 26% after precipitation. These conditions help E. koreanum tolerate a certain level of water scarcity, resulting in increased expression of flavonoid-related genes and ultimately enhancing the quality of the herb.

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Physiological and transcriptome analyses highlight multiple pathways involved in drought stress in Medicago falcata

Cited by 6 publications

References 57 publications

Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics

Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics

Transcriptomic Analysis of the Effect of Pruning on Growth, Quality, and Yield of Wuyi Rock Tea

Transcriptome Analysis on the Quality of Epimedium koreanum in Different Soil Moisture Conditions at Harvesting Stage

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