Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics

Dong, Shoukun; Wang, Xin; Li, Xiaomei; Tian, Yumei; Zhou, Xinyu; Qu, Zhipeng; Wang, Xiyue; Liu, Lijun

doi:10.3390/plants12102037

Cited by 3 publications

(4 citation statements)

References 51 publications

(50 reference statements)

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“…The PPI is involved in almost all the important biological processes within the living body and play a crucial role in the fundamental life processes of the cell [30]. Highly aggregated proteins often exhibit similar functions [31], while proteins with a high degree of connectivity may be key points for influencing metabolic reactions [27]. The DEPs in each comparison group were compared with the STRING database, and protein interaction network maps were drawn by screening the proteins with closer interactions (Figures 8-11).…”

Section: Ppi Network Analysis Of Deps In Different Samplesmentioning

confidence: 99%

Investigation of Acid Tolerance Mechanism of Acetobacter pasteurianus under Different Concentrations of Substrate Acetic Acid Based on 4D Label-Free Proteomic Analysis

Li,

Wang,

et al. 2023

Foods

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Acetobacter pasteurianus is always used to brew vinegar because of its ability of producing and tolerating a high concentration of acetic acid. During vinegar fermentation, initial acetic acid contributes to acetic acid accumulation, which varies with initial concentrations. In this study, to investigate the mechanisms of tolerating and producing acetic acid of Acetobacter pasteurianus under different concentrations of substrate acetic acid, four-dimensional label-free proteomic technology has been used to analyze the protein profiles of Acetobacter pasteurianus at different growth stages (the lag and exponential phases) and different substrate acetic acid concentrations (0%, 3%, and 6%). A total of 2093 proteins were quantified in this study. The differentially expressed proteins were majorly involved in gene ontology terms of metabolic processes, cellular metabolic processes, and substance binding. Under acetic acid stress, strains might attenuate the toxicity of acetic acid by intensifying fatty acid metabolism, weakening the tricarboxylic acid cycle, glycerophospholipid and energy metabolism during the lag phase, while strains might promote the assimilation of acetic acid and inter-conversion of substances during the exponential phase by enhancing the tricarboxylic acid cycle, glycolysis, pyruvate, and energy metabolism to produce and tolerate acid. Besides, cell cycle regulation and protein translation might be potential acid tolerance pathways under high acid stress. The result contributes to the exploration of new potential acid tolerance mechanisms in Acetobacter pasteurianus from four-dimensional label-free relative quantitative proteomics analysis.

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Section: Ppi Network Analysis Of Deps In Different Samplesmentioning

confidence: 99%

Investigation of Acid Tolerance Mechanism of Acetobacter pasteurianus under Different Concentrations of Substrate Acetic Acid Based on 4D Label-Free Proteomic Analysis

Li,

Wang,

et al. 2023

Foods

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“…MC regulates a wide range of biological activities in various crops [ 58 ]. In response to abiotic stresses, MC decreased vegetative growth while increasing crop yield in soybean and cotton under drought stress [ 40 , 59 ].…”

Section: Biosynthesis Signaling and Transduction Pathways Of MC And Mtmentioning

confidence: 99%

“…A previous study demonstrated that ABA is the primary hormone that promotes leaf senescence under abiotic stress in different crops [ 128 ]. The content of ABA and its biosynthesis genes gradually increased during leaf senescence in cotton plants [ 58 ]. MT reduced the upregulation of ABA biosynthesis genes, NCED1 and NCED2 , and their accumulation in cotton [ 129 ].…”

Section: MC and Mt Relevant Gene Involved In Cotton Growthmentioning

confidence: 99%

The Roles of Mepiquate Chloride and Melatonin in the Morpho-Physiological Activity of Cotton under Abiotic Stress

Wu,

Liu,

et al. 2023

IJMS

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Cotton growth and yield are severely affected by abiotic stress worldwide. Mepiquate chloride (MC) and melatonin (MT) enhance crop growth and yield by reducing the negative effects of abiotic stress on various crops. Numerous studies have shown the pivotal role of MC and MT in regulating agricultural growth and yield. Nevertheless, an in-depth review of the prominent performance of these two hormones in controlling plant morpho-physiological activity and yield in cotton under abiotic stress still needs to be documented. This review highlights the effects of MC and MT on cotton morpho-physiological and biochemical activities; their biosynthetic, signaling, and transduction pathways; and yield under abiotic stress. Furthermore, we also describe some genes whose expressions are affected by these hormones when cotton plants are exposed to abiotic stress. The present review demonstrates that MC and MT alleviate the negative effects of abiotic stress in cotton and increase yield by improving its morpho-physiological and biochemical activities, such as cell enlargement; net photosynthesis activity; cytokinin contents; and the expression of antioxidant enzymes such as catalase, peroxidase, and superoxide dismutase. MT delays the expression of NCED1 and NCED2 genes involved in leaf senescence by decreasing the expression of ABA-biosynthesis genes and increasing the expression of the GhYUC5, GhGA3ox2, and GhIPT2 genes involved in indole-3-acetic acid, gibberellin, and cytokinin biosynthesis. Likewise, MC promotes lateral root formation by activating GA20x genes involved in gibberellin catabolism. Overall, MC and MT improve cotton’s physiological activity and antioxidant capacity and, as a result, improve the ability of the plant to resist abiotic stress. The main purpose of this review is to present an in-depth analysis of the performance of MC and MT under abiotic stress, which might help to better understand how these two hormones regulate cotton growth and productivity.

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“…The use of MC under salt stress can significantly increase dry weight, fresh weight, glycine betaine content, and proline and phenolic compounds in cotton [ 12 ]. Additionally, MC enhanced the GSH-ASA and Calvin cycles of soybeans during drought stress to lessen oxidative damage and preserve crop development [ 13 ]. Therefore, MC has considerable application potential in the regulation of crop yield and stress resistance.…”

Section: Introductionmentioning

confidence: 99%

The application potential of mepiquat chloride in soybean: improvement of yield characteristics and drought resistance

Wang,

Zhao,

Wei

et al. 2024

BMC Plant Biol

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Background Drought can result in yield losses, the application of plant growth regulators is an effective measure to improve drought resistance and yield. The objective of the study was to explore the application potential of mepiquat chloride (MC) in regulating soybean yield and drought resistance. Methods In this study, a three-year field experiment was designed and combined with drought experiments to measure the yield of popularized varieties during 2021–2022 and drought-resistant and drought-sensitive varieties were selected, and planted in the field in 2023. Results MC increased the yield of HN84 and HN87 for two consecutive years from 2021 to 2022 and improved their physiological characteristics under field conditions. Under M200 treatment, the yield of HN84 increased by 6.93% and 9.46%, and HN87 increased by 11.11% and 15.72%. Different concentrations of MC have different effects on soybeans. The maximum increase of SOD, POD and proline in HN84 under M400 treatment reached 71.92%, 63.26% and 71.54%, respectively; the maximum increase of SOD, POD and proline in HN87 under M200 treatment reached 21.96%, 93.49% and 40.45%, respectively. In 2023, the foliar application of MC improved the physiological characteristics of HN44 and HN65 under drought-stress conditions. On the eighth day of drought treatment, compared to the drought treatment, the leaf and root dry weight of HN44 under M100 treatment increased by 17.91% and 32.76%, respectively; the dry weight of leaves and roots of HN65 increased by 20.74% and 29.29% under M200 treatment, respectively. MC also reduced malondialdehyde (MDA) content, decreased antioxidant enzyme activity and proline content. In addition, different concentrations of MC increased the chlorophyll fluorescence parameters (Fs, Fv/Fm, YII, and SPAD). In the field, the plant height of the two varieties decreased significantly, the yield increased, the number of two-grain and three-grain pods increased, and the stem length at the bottom and middle decreased with MC induction. Conclusions The application of 100–200 mg/L MC effectively improved drought resistance and increased yield. This study provided support for the rational application of MC in soybean production.

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Mechanism of Mepiquat Chloride Regulating Soybean Response to Drought Stress Revealed by Proteomics

Cited by 3 publications

References 51 publications

Investigation of Acid Tolerance Mechanism of Acetobacter pasteurianus under Different Concentrations of Substrate Acetic Acid Based on 4D Label-Free Proteomic Analysis

Investigation of Acid Tolerance Mechanism of Acetobacter pasteurianus under Different Concentrations of Substrate Acetic Acid Based on 4D Label-Free Proteomic Analysis

The Roles of Mepiquate Chloride and Melatonin in the Morpho-Physiological Activity of Cotton under Abiotic Stress

The application potential of mepiquat chloride in soybean: improvement of yield characteristics and drought resistance

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