Physiological and Serological Comparisons Among Strains of<i>Mycoplasma granularum</i>and<i>Mycoplasma laidlawii</i>

Tully, Joseph G.; Razin, Shmuel

doi:10.1128/jb.95.5.1504-1512.1968

Cited by 40 publications

(23 citation statements)

References 27 publications

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“…This study confirms evidence (19,20) that Mycoplasma serotypes have a wide range of biophysical and biochemical characteristics. Such differences are of value in selective primary isolation of pathogenic species as has been done with M. pneumoniae (3), in purification of mixed cultures, and in taxonomic grouping.…”

Section: Discussionsupporting

confidence: 88%

Identification of Mycoplasmatales: Procedures for Both Characterization and Purification 1

Barber¹,

Fabricant²

1971

Applied Microbiology

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Procedures with potential value for identification and purification of Mycoplasma were applied to over 240 cultures representing 11 of the 12 known avian serotypes. Growth was tested at pH 5.5 and 9.5, at 25 and 42 C, in serum-free media, and in the presence of (i) 1% bile salts, (ii) 3% NaCl, and (iii) 0.02% methylene blue. One avian Mycoplasma serotype grew in broth containing 1% bile salts. Two of 11 avian serotypes were resistant to 0.02% methylene blue. A number of Mycoplasma strains grew at 42 C or in apH 9.5 medium. Usefulness of these procedures for purifying cultures of Mycoplasma is discussed.

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Section: Discussionsupporting

confidence: 88%

Identification of Mycoplasmatales: Procedures for Both Characterization and Purification 1

Barber¹,

Fabricant²

1971

Applied Microbiology

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“…All of the A . laidlawii strains had previously been shown to be related to the type strain PG-8 of this organism by growth inhibition (Clyde, 1964;Orning et al, 1978) and immunofluorescence (DelGiudice et af., 1967) serological techniques (Tully 1973;Tully & Razin, 1968). Several of the A .…”

Section: Introductionmentioning

confidence: 99%

“…Those not included and representing more recent isolates, were identified by growth inhibition and immunofluorescence tests with antiserum prepared to the H86N strain of A . axanthum, using procedures described earlier (Tully 1973;Tully & Razin, 1968).…”

Section: Introductionmentioning

confidence: 99%

Intraspecies Genetic Relatedness among Strains of Acholeplasma laidlawii and of Acholeplasma axanthum by Nucleic Acid Hybridization

Stephens¹,

Aulakh²,

Rose³

et al. 1983

Microbiology

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This study compares the intraspecies genetic relatedness among strains of two established species of Acholeplasma. Radiolabelled DNA probes were prepared from three strains of Acholeplasma laidlawii and two strains of Acholeplasma axanthum, by using the nick translation method. The labelled DNA probes of these two strains were hybridized to an excess of unlabelled DNA from 12 strains of Acholeplasma laidlawii and from six strains of Acholeplasma axanthum, respectively. Nucleic acid hybridization analyses showed a wide variation among strains within each of the two established species, ranging from 48 to 100% homology. The results demonstrate that strains isolated from diverse hosts and habitats within a given species of Acholeplasma exhibit extensive genotypic variations.

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“…Yet, this important characteristic has directly been tested and confirmed for only a few of the over 30 Mycoplasma species established so far (2, 5,7,8,12,13,16,20). The necessity for testing the requirement for cholesterol in all the established Mycoplasma species and in proposed new species became apparent with the recent discovery of mycoplasmas which do not require cholesterol for growth and which differ from the long-known sterol-nonrequiring M. laidlawii strains (21,22). Edward and Freundt (3) have proposed the division of the Mycoplasmatales into two families: one for those dependent on sterol for growth, and the other for those not dependent on sterol.…”

mentioning

confidence: 99%

Cholesterol Requirement of Mycoplasmas

1970

Self Cite

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Cholesterol requirement for growth of mycoplasmas was tested in a serum-free medium supplemented with albumin, L-arginine, palmitic acid, and various concentrations of cholesterol dissolved in Tween 80. In cases in which Tween 80 was shown to inhibit growth, the test medium was supplemented with cholesterol dissolved in ethanol. Of the 31 species examined, all but Mycoplasma laidlawii, M. granularum, and Mycoplasma species strain S-743 exhibited a growth response to cholesterol. No requirement for cholesterol could be shown with the stable L-phase variants of Streptobacillus moniliformis and Proteus species. The results provide experimental support for the view that the large majority of the established Mycoplasma species require cholesterol for growth. strain designation Mycoplasma salivarium PG-20 M. fermentans PG-18 M. pneumoniae FH M. orale 1 CH 19299 M. orale 2 CH 20247 M. orale 3 DC 333 M. lipophilum MaBy Mycoplasma sp. Navel M. neurolyticum type A M. pulmonis PG-34 M. arthritidis PG-6 M. bovigenitalium PG-11 M. bovirhinis PG-43 M. hyorhinis BTS-7 M. mycoides var. capri PG-3 M. canis PG-14 M. maculosum PG-15 M. edwardii PG-24 M. felis Cat 27 M. gallisepticum S6 M. gallinarum PG-16 M. iners 640 M. meleagridis 886 M. anatis 1340 M. laidlawii PG-8 M. granularum BTS-39 Mycoplasma sp. S-743

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Physiological and Serological Comparisons Among Strains ofMycoplasma granularumandMycoplasma laidlawii

Cited by 40 publications

References 27 publications

Identification of Mycoplasmatales: Procedures for Both Characterization and Purification 1

Identification of Mycoplasmatales: Procedures for Both Characterization and Purification 1

Intraspecies Genetic Relatedness among Strains of Acholeplasma laidlawii and of Acholeplasma axanthum by Nucleic Acid Hybridization

Cholesterol Requirement of Mycoplasmas

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