Identification of Mycoplasmatales: Procedures for Both Characterization and Purification 1

Abstract

Procedures with potential value for identification and purification of Mycoplasma were applied to over 240 cultures representing 11 of the 12 known avian serotypes. Growth was tested at pH 5.5 and 9.5, at 25 and 42 C, in serum-free media, and in the presence of (i) 1% bile salts, (ii) 3% NaCl, and (iii) 0.02% methylene blue. One avian Mycoplasma serotype grew in broth containing 1% bile salts. Two of 11 avian serotypes were resistant to 0.02% methylene blue. A number of Mycoplasma strains grew at 42 C or in apH 9.5 medium. Usefulness of these procedures for purifying cultures of Mycoplasma is discussed.

Differentiation of Sterol-Requiring from Sterol-Nonrequiring Mycoplasmas by Amphotericin B

“…The inhibition was somewhat less with M. mycoides var capri and M. anatis. Their lower sensitivity to the drug is in accordance with their ability to grow for several subcultures in the absence of serum (2,10).…”

Reduction of Hydrogen Peroxide Accumulation and Toxicity by a Catalase from Mycoplasma iowae

“…M. iowae has a terminal organelle with an internal cytoskeleton; this organelle attaches to host cells and is the leading cell pole during gliding motility [6] , [8] . M. iowae is one of two known avian mycoplasmas, along with Mycoplasma lipofaciens , that can obtain energy from catabolism of both glucose and arginine [9] , [10] . Despite considerable understanding of disease pathology resulting from M. iowae infection, as well as some knowledge about its cellular biology and biochemistry, the virulence factors of M. iowae are unidentified.…”