Physiological and phylogenetic variability of Mexican Metarhizium strains

Brunner-Mendoza, Carolina; Moonjely, Soumya; Reyes-Montes, María del Roció; Toriello, Conchita; Bidochka, Michael

doi:10.1007/s10526-017-9839-3

Cited by 11 publications

(6 citation statements)

References 33 publications

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“…Our results showed 2 major clusters: Group I that included all strains from Mexico isolated from Hemiptera in sugar cane fields, and Group II with all reference strains obtained from different hosts and geographic origins. Strains from Mexico formed a cluster with high support values from both RAPD data and ITS sequences analysis, suggesting that the existing populations of M. anisopliae are in a process of divergence and the resulting linages are undergoing a process of speciation, as shown in previous studies with the same strains identified as a subclade (Mani 2) within M. anisopliae (Brunner-Mendoza et al 2017) revealing a greater number of phylogenetic species. Besides, Fungaro et al (1996) demonstrated that M. anisopliae strains are extremely diverse but insect strains present a moderate degree of variation, suggesting the development of host specificity.…”

Section: Discussionsupporting

confidence: 63%

“…In Mexico, M. anisopliae is the main agent used against spittlebugs (Hemiptera: Cercopidae) that affect sugarcane (one of Mexico's most profitable crops) and cattle pastures (Senasica, 2017). Brunner-Mendoza et al (2017) and Rezende et al (2015) have demonstrated the variability of the genus Metarhizium. The internal transcribed spacer (ITS) region of nuclear ribosomal DNA was adopted as the primary fungal barcode marker by the Consortium for the Barcode of Life (Schoch et al, 2012), due to its easy amplification and high yield, providing positive results in most phyla.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains

et al. 2019

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Metarhizium anisopliae is an anamorphic fungus widely studied due to its use as a biological control agent. In Mexico, it is mainly used as an integrated management strategy to control populations of spittlebugs (Hemiptera: Cercopidae) that affect sugarcane fields and cattle pastures. In this study, 13 strains isolated from Aeneolamia spp. (Hemiptera: Cercopidae) from the main areas of sugarcane production of Mexico and 11 reference strains of Metarhizium from different insect hosts and geographic origins were evaluated based on their conidial dimensions, thermotolerance at 15, 25, 30, and 35 ºC, random amplification of polymorphic DNA (RAPD) patterns using a combined analysis with 14 different oligonucleotides and diversity estimators, and a phylogenetic analysis with the ribosomal RNA internal transcribed spacer (ITS) region. All strains used in this study showed typical morphological characteristics corresponding to M. anisopliae and were able to grow at 25 and 30 ºC with restricted growth at low (15 ºC) and high temperatures (35 ºC). Polymorphism analysis clustered all strains from Aeneolamia sp. in one welldefined group with low variability among them. Phylogenetic relationships based on nuclear ribosomal ITS region sequences recovered a subclade within M. anisopliae formed by 10 of the Mexican strains.

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Section: Discussionsupporting

confidence: 63%

Section: Introductionmentioning

confidence: 99%

Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains

et al. 2019

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“…used against different hosts (e.g. Brunner-Mendoza et al, 2017;Dotaona et al, 2015;Oreste et al, 2012;Resquín-Romero et al, 2020), and in our study, such variation was also detected when fungal treatments were applied through the spray tower, and for the bait technique. Ondiaka et al (2008) reported intraspecies variability for M. anisopliae and Beauveria bassiana tested against adults of the sweet potato weevil Cylas puncticollis (concentration: 10 7 conidia mL -1 ), and stated that strain selection is needed for biological control.…”

Section: Discussionsupporting

confidence: 74%

Niche adaptations and plasticity within the fungal entomopathogenic genus >i<Metarhizium>/i< in Brazil

Couceiro¹

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A special thanks to my advisors, Prof. Dr. Italo Delalibera Jr. and Prof. Dr. Nicolai Vitt Meyling, for believing in me to conduct this research, for the opportunities and for the knowledge they shared with me during these years. Your guidance was essential to this study.A special thanks to Prof. Dr. Henrik Hjarvard de Fine Licht, my co-advisor in Copenhagen, which provided an enormous support in the aspects involving molecular biology.Your guidance gave me the support needed to increase my understanding about the area and to conduct the research at SOBI.To my parents, João Bosco Couceiro da Silva and Telma Maria da Cruz Couceiro, you are an inspiration for me. Since day 1, you were rooting for my success. I would not be where I am without your constant support, motivation and love. I want to thank the lab technicians Solange Aparecida Vieira Barros (ESALQ), Didde Hedegaard Sørensen (SOBI) and Karen Rysbjerg Jensen (SOBI), who instructed and helped me executing parts of this project. Thank you for sharing your knowledge.

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“…These include biological process associated to SMs, transport of amino and organic acid and carbohydrate active enzymes (CAzymes) that act on different substrates such as chitin, chitosan and trehalose, from arthropods, and cellulose, peptidoglycan, and xylan from plants. Indeed, M. humberi can explore different niches, such as free-living in soil ( Rezende et al 2015 ; Iwanicki et al 2019 ; Riguetti Zanardo Botelho et al 2019 ), infecting diverse orders of insects ( Lopes et al 2014 ; Rezende et al 2015 ; Brunner-Mendoza et al 2017 ) and establishing associations with plants ( Canassa et al 2019c ; Lira et al 2020 ; Siqueira et al 2020 ) and should be considered a broad host range species. On the other hand, narrow host range species associated only with specific insect orders are found at lower frequencies in soil and associated with plants.…”

Section: Discussionmentioning

confidence: 99%

Genomic signatures and insights into host niche adaptation of the entomopathogenic fungus Metarhizium humberi

Iwanicki¹,

Botelho²,

Klingen

et al. 2021

G3 Genes|Genomes|Genetics

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The genus Metarhizium is composed of species used in biological control programes of agricultural pests worldwide. This genus includes common fungal pathogen of many insects and mites and endophytes that can increase plant growth. Metarhizium humberi was recently described as a new species. This species is highly virulent against some insect pests and promotes growth in sugarcane, strawberry, and soybean crops. In the present study, we sequenced the genome of M. humberi, isolate ESALQ1638, and performed a functional analysis to determine its genomic signatures and highlight the genes and biological processes associated with its lifestyle. The genome annotation predicted 10633 genes in M. humberi, of which 92.0% are assigned putative functions, and ∼17% of the genome was annotated as repetitive sequences. We found that 18.5% of the M. humberi genome is similar to experimentally validated proteins associated with pathogen-host interaction. Compared to the genomes of eight Metarhizium species, the M. humberi ESALQ1638 genome revealed some unique traits that stood out, e.g.,, more genes functionally annotated as polyketide synthases (PKs), overrepresended GO-terms associated to transport of ions, organic and amino acid, a higher percentage of repetitive elements, and higher levels of RIP-induced point mutations. The M. humberi genome will serve as a resource for promoting studies on genome structure and evolution that can contribute to research on biological control and plant biostimulation. Thus, the genomic data supported the broad host range of this species within the generalist PARB clade and suggested that M. humberi ESALQ1638 might be particularly good at producing secondary metabolites and might be more efficient in transporting amino acids an organics compounds.

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Physiological and phylogenetic variability of Mexican Metarhizium strains

Cited by 11 publications

References 33 publications

Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains

Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains

Niche adaptations and plasticity within the fungal entomopathogenic genus >i<Metarhizium>/i< in Brazil

Genomic signatures and insights into host niche adaptation of the entomopathogenic fungus Metarhizium humberi

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