Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains

Navarro-Barranco, Hortensia; Brunner-Mendoza, Carolina; Reyes-Montes, María del Roció; Duarte-Escalante, Esperanza; Toriello, Conchita

doi:10.22201/ib.20078706e.2019.90.2643

Cited by 2 publications

(2 citation statements)

References 25 publications

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“…Los marcadores RAPD se han utilizado ampliamente en varios estudios sobre diversidad genética de hongos entomopatógenos, incluyendo a Metarhizium (Bidochka et al, 1994;Coob y Clarkson, 1993;Fegan et al, 1993;Leal et al, 1994;Navarro-Barranco et al, 2019). Esta técnica provee varias ventajas y se ha demostrado que sus resultados son similares a los determinador por secuencias ITS entre aislados nativos de México y aislados referenciales de Metarhizium (Navarro-Barranco et al, 2019). También fue capaz para detectar entre aislados Chilenos de Metarhizium alto nivel de diversidad genética con al menos cuatro oligonucleótidos decámeros RAPD (Becerra-Velazquez et al 2007), mientras que en el presente estudio se emplearon nueve oligonucleótidos, los cuales fueron capaces de diferenciar genéticamente a aislados de Metarhizium provenientes de suelo y de insectos.…”

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DIVERSIDAD GENÉTICA DE Metarhizium anisopliae AISLADOS DE INSECTOS Y AGROECOSISTEMAS

Ramirez-Milanes¹,

Lezama-Gutiérrez²,

Sánchez-Rangel³

et al. 2021

Trop Subtrop Agroecosyst

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Background. The genus Metarhizium is integrated of various species, they have a wide geographic distribution and are considered one of the best biological control agents for pests insect. Objective. To determine the genetic diversity of Metarhizium strains isolated from different insects and agroecosystems using RAPD markers. Methodology. Soil was collected from different agroecosystems (Mexican lemon, grapefruit, sugar cane, mango), insects Diatraea saccharalis (Lepidoptera: Crambidae), Diatraea magnifactella (Lepidoptera: Crambidae), Schistocerca piceifrons piceifrons (Orthoptera: Acridylus), Macrodactylus murinus (Coleoptera: Scarabaeidae), Spodoptera frugiperda (Lepidoptera: Noctuidae) and the mite Boophilus microplus (Acari: Ixodidae) to isolate Metarhizium strains, the isolates were analyzed through random amplified polymorphic DNA (RAPD) markers. Results. The RAPD markers were highly informative and polymorphic. The generated dendrogram was made up of four clusters (A-D); cluster A consisted of 11 soil isolates, one from D. magnifactella and two from D. saccharalis. In cluster B one of D. saccharalis and one of soil. On the other hand, in cluster C were that B. microplus and one of soil. While cluster D was integrated by the S. frugiperda, M. murinus and S. piceifrons isolates. Implications. The determination of the genetic diversity between Metarhizium isolates obtained from insects and agroecosystems, generates a contribution on the habitat that will allow the development of strategies to carry out an adequate biological control of pest insects. Conclusion. The analyzes using the RAPD markers indicated that there is a genetic diversity among the Metarhizium isolates collected from different insects and agroecosystems.

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Section: Discussionunclassified

DIVERSIDAD GENÉTICA DE Metarhizium anisopliae AISLADOS DE INSECTOS Y AGROECOSISTEMAS

Ramirez-Milanes¹,

Lezama-Gutiérrez²,

Sánchez-Rangel³

et al. 2021

Trop Subtrop Agroecosyst

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Virulence of Metarhizium isolated from infected Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) larvae around coconut plantations in East Java, Indonesia

Anur

Soeharto

Addy

et al. 2022

Egypt J Biol Pest Control

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Background Metarhizium is one of the entomopathogenic fungi (EPF) that has been widely reported as a useful agent for controlling the coconut rhinoceros beetle (CRB), Oryctes rhinoceros L. (Coleoptera: Scarabaeidae). Application of this fungus as a biopesticide is influenced by regional environmental conditions that affect the pathogenic activity against the targeted pest. Several studies have proven that the native fungal isolates have strong pathogenic activity than EPF introduced from other regions. The identification of local EPF species is a strategic approach to develop potential biopesticides with standard properties including host specificity, climate suitability, and significantly suppressing the target pest population. This study aimed to identify Metarhizium species isolated from infected CRB larvae with high pathogenicity to host pests in East Java, Indonesia. Results Thirteen isolates were obtained which were divided into 4 clades based on phylogenetic analysis by the ITS rDNA region, namely M. anisopliae var. lepidiotae, M. anisopliae var. anisopliae, M. brunneum, and M. majus. Identified Metarhizium species exhibited varied sizes of conidia, but fell within the size ranges reported in previous studies. Interestingly, the isolate MaSi produced conidial lengths well above the range of conidial sizes recorded and placed these isolates (M. anisopliae) in the MGT clade, known as MALC (M. anisopliae s.l. with large conidia). The present investigation reported that isolates MaLe, MaMa, MaWa, and MaSi produced higher mortality values than other isolates, supporting that M. majus and MALC had higher pathogenicity against O. rhinoceros larvae than other Metarhizium species. Neighbor-joining analysis showed a close resemblance between the isolate MaMa and the strain ARSEF 1946, which was isolated from the CRB. In addition, the isolate MaMa had the highest virulence against O. rhinoceros larval cadaver with a faster lethal time (for 50% mortality). This result indicated a possible relationship between phylogenetic status or DNA sequence polymorphisms with Metarhizium pathogenicity and host specificity. Conclusions The local species of Metarhizium have been isolated from infected CRB larvae, and M. majus isolates exhibited high pathogenicity against O. rhinoceros larvae. The close similarity of M. majus isolates and CRB-isolated strains suggests a possible relationship between pathogenicity and host specificity with phylogenetic status or DNA sequence polymorphisms.

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Phenotypic and molecular analysis of Mexican Metarhizium anisopliae strains

Cited by 2 publications

References 25 publications

DIVERSIDAD GENÉTICA DE Metarhizium anisopliae AISLADOS DE INSECTOS Y AGROECOSISTEMAS

DIVERSIDAD GENÉTICA DE Metarhizium anisopliae AISLADOS DE INSECTOS Y AGROECOSISTEMAS

Virulence of Metarhizium isolated from infected Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) larvae around coconut plantations in East Java, Indonesia

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