Physiological and Pathophysiological Functions of SIRT1

Wójcik, Marzena; Mac-Marcjanek, Katarzyna; Woźniak, Lucyna A.

doi:10.2174/1389557510909030386

Cited by 38 publications

(27 citation statements)

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“…The SIRT1 gene plays an important role in glucose and lipid metabolism [32]. One potential explanation for the significant association observed between rs3758391 and T2D, but not MS, is that this polymorphism, which is located in the promoter region of the SIRT1 gene, could alter the function of SIRT1 as a regulator of gluconeogenesis and insulin secretion [32,[60][61][62][63].…”

Section: Discussionmentioning

confidence: 99%

Candidate gene association study conditioning on individual ancestry in patients with type 2 diabetes and metabolic syndrome from Mexico City

Cruz

Valladares‐Salgado

García-Mena

et al. 2010

Diabetes Metabolism Res

100

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Section: Discussionmentioning

confidence: 99%

Candidate gene association study conditioning on individual ancestry in patients with type 2 diabetes and metabolic syndrome from Mexico City

Cruz

Valladares‐Salgado

García-Mena

et al. 2010

Diabetes Metabolism Res

100

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“…3 Sirtuin activities are thought to mediate the beneficial effects of calorie restriction (CR), 4 which increases lifespan in a variety of organisms from yeast to mammals. 5–7 This compelling idea has been reinforced by a growing body of evidence that sirtuins are involved in regulating numerous physiologic processes such as adipogenesis, 8 fatty acid catabolism, 9 mitochondrial biogenesis, 10 and insulin secretion. 11,12 In addition to being the “master regulator” of metabolic programming, sirtuins have also been implicated in various pathological conditions and have been shown to be forceful potentiators and sustainers of diseases such as cancer.…”

mentioning

confidence: 99%

Development of Activity-Based Chemical Probes for Human Sirtuins

et al. 2018

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Sirtuins consume stoichiometric amounts of nicotinamide adenine dinucleotide (NAD+) to remove an acetyl group from lysine residues. These enzymes have been implicated in regulating various cellular events and have also been suggested to mediate the beneficial effects of calorie restriction (CR). However, controversies on sirtuin biology also peaked during the past few years because of conflicting results from different research groups. This is partly because these enzymes have been discovered recently and the intricate interaction loops between sirtuins and other proteins make the characterization of them extremely difficult. Current molecular biology and proteomics techniques report protein abundance rather than active sirtuin content. Innovative chemical tools that can directly probe the functional state of sirtuins are desperately needed. We have obtained a set of powerful activity-based chemical probes that are capable of assessing the active content of sirtuins in model systems. These probes consist of a chemical “warhead” that binds to the active site of active enzyme and a handle that can be used for the visualization of these enzymes by fluorescence. In complex native proteome, the probes can selectively “highlight” the active sirtuin components. Furthermore, these probes were also able to probe the dynamic change of sirtuin activity in response to cellular stimuli. These chemical probes and the labeling strategies will provide transformative technology to allow the direct linking of sirtuin activity to distinct physiological processes. They will create new opportunities to investigate how sirtuins provide health benefits in adapting cells to environmental cues and provide critical information to dissect sirtuin regulatory networks.

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“…Over the past years, SIRT1 has been considered as an essential modulator of glucose and lipid homeostasis in different metabolic tissues, including liver, skeletal muscle, and white adipose tissue, as well as insulin secretion by pancreatic b cells and inflammation [9,10]. Therefore, establishing whether SIRT1 is related to PI3-K delta in GDM was of great interest to us.…”

Section: Prace Oryginalnementioning

confidence: 99%

“…Moreover, we sought to determine the relationship between the expression of PI3KCD and Sirtuin 1 (SIRT1) in leukocytes of the GDM patients since SIRT1 has received significant attention as a key NAD+-dependent deacetylase linking caloric restriction to mammalian cell life span, insulin secretion, and glucose/lipid metabolism [9,10].…”

Section: Introductionmentioning

confidence: 99%

Związek podwyższonej ekspresji leukocytarnej kinazy 3-fosfatydyloinozytolu delta z cukrzycą ciążową (GDM)

Wójcik¹,

Mac-Marcjanek²,

Woźniak³

et al. 2014

Endokrynologia Polska

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Introduction: An increasing body of evidence has linked diabetes to inflammation. The phosphatidylinositol 3-kinase delta (PI3-K delta), a member of the PI3K class IA family, has been implicated in the regulation of inflammation since it is predominantly expressed in leukocytes. To date, no information has been available on the relationship of leukocyte PI3-K delta with gestational diabetes mellitus (GDM). Therefore, the aim of this study was to investigate changes in leukocyte PIK3CD mRNA expression in GDM women and, in turn, to correlate them with anthropometric and metabolic parameters of patients. Additionally, an association between leukocyte mRNA expression of PIK3CD and Sirtuin 1 (SIRT1) was determined. Material and methods: Blood samples from women with normal glucose tolerance (NGT; n = 43) and GDM (n = 132) at 24-33 weeks of gestation were collected. After isolating leukocytes from the blood, quantitative real time PCR (qRT-PCR) was performed to determine PIK3CD gene expression in these cells. Univariate regression analyses were used to assess an association of leukocyte PIK3CD mRNA level with clinical characteristics of patients as well as with leukocyte SIRT1 mRNA expression. Results: Leukocyte PIK3CD mRNA was increased by 1.98-fold in the GDM v. NGT subjects and inversely correlated with low-density lipoprotein cholesterol (LDL-C) and total cholesterol (TC) in diabetic pregnancy. There were also significant positive correlations of leukocyte PIK3CD mRNA with plasma glucose concentration at 2h of 75 g oral glucose tolerance test (OGTT) and SIRT1 mRNA in the whole study population (both P < 0.05). Conclusions: GDM is accompanied by leukocyte PIK3CD overexpression associated with reduced plasma LDL-C and TC levels, as well as with hyperglycaemia and elevated leukocyte SIRT1 mRNA. (Endokrynol Pol 2014; 65 (1): 17-24)

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Physiological and Pathophysiological Functions of SIRT1

Cited by 38 publications

References 0 publications

Candidate gene association study conditioning on individual ancestry in patients with type 2 diabetes and metabolic syndrome from Mexico City

Candidate gene association study conditioning on individual ancestry in patients with type 2 diabetes and metabolic syndrome from Mexico City

Development of Activity-Based Chemical Probes for Human Sirtuins

Związek podwyższonej ekspresji leukocytarnej kinazy 3-fosfatydyloinozytolu delta z cukrzycą ciążową (GDM)

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